Experiment: Carbon source 2-Deoxyadenosine 5-monophosphate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01345 and RR42_RS01350 are separated by 53 nucleotides RR42_RS01350 and RR42_RS01355 overlap by 1 nucleotides RR42_RS01355 and RR42_RS01360 are separated by 48 nucleotides RR42_RS01360 and RR42_RS01365 are separated by 4 nucleotides
RR42_RS01345: RR42_RS01345 - CoA-binding protein, at 305,599 to 307,710
_RS01345
RR42_RS01350: RR42_RS01350 - biotin carboxylase, at 307,764 to 309,137
_RS01350
RR42_RS01355: RR42_RS01355 - biotin attachment protein, at 309,137 to 309,349
_RS01355
RR42_RS01360: RR42_RS01360 - phenylacetic acid degradation protein, at 309,398 to 309,826
_RS01360
RR42_RS01365: RR42_RS01365 - hypothetical protein, at 309,831 to 310,271
_RS01365
Position (kb) 307
308
309
310 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 307.038 kb on - strand, within RR42_RS01345 at 307.309 kb on + strand, within RR42_RS01345 at 307.310 kb on - strand, within RR42_RS01345 at 307.384 kb on + strand, within RR42_RS01345 at 307.384 kb on + strand, within RR42_RS01345 at 307.385 kb on - strand, within RR42_RS01345 at 307.434 kb on - strand, within RR42_RS01345 at 307.643 kb on + strand at 307.643 kb on + strand at 307.644 kb on - strand at 307.739 kb on - strand at 307.739 kb on - strand at 307.872 kb on + strand at 307.929 kb on + strand, within RR42_RS01350 at 308.775 kb on + strand, within RR42_RS01350 at 308.776 kb on - strand, within RR42_RS01350 at 308.776 kb on - strand, within RR42_RS01350 at 308.825 kb on - strand, within RR42_RS01350 at 308.896 kb on - strand, within RR42_RS01350 at 308.896 kb on - strand, within RR42_RS01350 at 308.898 kb on + strand, within RR42_RS01350 at 308.898 kb on + strand, within RR42_RS01350 at 308.898 kb on + strand, within RR42_RS01350 at 309.008 kb on + strand at 309.120 kb on - strand at 309.159 kb on + strand, within RR42_RS01355 at 309.159 kb on + strand, within RR42_RS01355 at 309.269 kb on + strand, within RR42_RS01355 at 309.270 kb on - strand, within RR42_RS01355 at 309.348 kb on - strand at 309.434 kb on + strand at 309.649 kb on + strand, within RR42_RS01360 at 309.649 kb on + strand, within RR42_RS01360 at 309.650 kb on - strand, within RR42_RS01360 at 309.650 kb on - strand, within RR42_RS01360 at 309.892 kb on + strand, within RR42_RS01365 at 310.063 kb on + strand, within RR42_RS01365
Per-strain Table
Position Strand Gene LocusTag Fraction Carbon source 2-Deoxyadenosine 5-monophosphate 5 mM remove 307,038 - RR42_RS01345 0.68 -1.0 307,309 + RR42_RS01345 0.81 -1.0 307,310 - RR42_RS01345 0.81 -1.7 307,384 + RR42_RS01345 0.85 -0.1 307,384 + RR42_RS01345 0.85 -2.9 307,385 - RR42_RS01345 0.85 +0.8 307,434 - RR42_RS01345 0.87 +0.1 307,643 + -0.7 307,643 + -0.7 307,644 - +0.2 307,739 - +0.7 307,739 - +0.2 307,872 + +2.8 307,929 + RR42_RS01350 0.12 +1.1 308,775 + RR42_RS01350 0.74 +1.0 308,776 - RR42_RS01350 0.74 -0.3 308,776 - RR42_RS01350 0.74 -1.4 308,825 - RR42_RS01350 0.77 +0.8 308,896 - RR42_RS01350 0.82 +0.7 308,896 - RR42_RS01350 0.82 +1.1 308,898 + RR42_RS01350 0.83 -1.4 308,898 + RR42_RS01350 0.83 +1.0 308,898 + RR42_RS01350 0.83 -0.1 309,008 + +0.7 309,120 - +0.9 309,159 + RR42_RS01355 0.10 +0.1 309,159 + RR42_RS01355 0.10 -0.5 309,269 + RR42_RS01355 0.62 +0.1 309,270 - RR42_RS01355 0.62 -0.4 309,348 - +0.3 309,434 + -0.3 309,649 + RR42_RS01360 0.59 -1.3 309,649 + RR42_RS01360 0.59 +1.1 309,650 - RR42_RS01360 0.59 -1.8 309,650 - RR42_RS01360 0.59 -0.5 309,892 + RR42_RS01365 0.14 +1.1 310,063 + RR42_RS01365 0.53 +0.1
Or see this region's nucleotide sequence