Experiment: Carbon source 2-Deoxyadenosine 5-monophosphate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01230 and RR42_RS01235 are separated by 86 nucleotides RR42_RS01235 and RR42_RS01240 are separated by 122 nucleotides
RR42_RS01230: RR42_RS01230 - acetoacetate decarboxylase, at 278,651 to 279,391
_RS01230
RR42_RS01235: RR42_RS01235 - 3-hydroxybutyrate dehydrogenase, at 279,478 to 280,260
_RS01235
RR42_RS01240: RR42_RS01240 - membrane protein, at 280,383 to 281,735
_RS01240
Position (kb)
279
280
281 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 278.696 kb on - strand at 278.831 kb on + strand, within RR42_RS01230 at 278.832 kb on - strand, within RR42_RS01230 at 278.873 kb on + strand, within RR42_RS01230 at 278.874 kb on - strand, within RR42_RS01230 at 278.874 kb on - strand, within RR42_RS01230 at 278.906 kb on + strand, within RR42_RS01230 at 278.906 kb on + strand, within RR42_RS01230 at 278.927 kb on + strand, within RR42_RS01230 at 278.927 kb on + strand, within RR42_RS01230 at 278.927 kb on + strand, within RR42_RS01230 at 278.928 kb on - strand, within RR42_RS01230 at 278.928 kb on - strand, within RR42_RS01230 at 278.942 kb on + strand, within RR42_RS01230 at 278.943 kb on - strand, within RR42_RS01230 at 278.983 kb on - strand, within RR42_RS01230 at 278.983 kb on - strand, within RR42_RS01230 at 279.034 kb on + strand, within RR42_RS01230 at 279.034 kb on + strand, within RR42_RS01230 at 279.056 kb on + strand, within RR42_RS01230 at 279.092 kb on + strand, within RR42_RS01230 at 279.092 kb on + strand, within RR42_RS01230 at 279.152 kb on + strand, within RR42_RS01230 at 279.153 kb on - strand, within RR42_RS01230 at 279.212 kb on + strand, within RR42_RS01230 at 279.212 kb on + strand, within RR42_RS01230 at 279.212 kb on + strand, within RR42_RS01230 at 279.213 kb on - strand, within RR42_RS01230 at 279.213 kb on - strand, within RR42_RS01230 at 279.335 kb on + strand at 279.336 kb on - strand at 279.355 kb on - strand at 279.380 kb on + strand at 279.778 kb on + strand, within RR42_RS01235 at 279.858 kb on + strand, within RR42_RS01235 at 279.858 kb on + strand, within RR42_RS01235 at 279.858 kb on + strand, within RR42_RS01235 at 279.858 kb on + strand, within RR42_RS01235 at 279.858 kb on + strand, within RR42_RS01235 at 279.859 kb on - strand, within RR42_RS01235 at 279.859 kb on - strand, within RR42_RS01235 at 279.859 kb on - strand, within RR42_RS01235 at 279.859 kb on - strand, within RR42_RS01235 at 279.859 kb on - strand, within RR42_RS01235 at 279.859 kb on - strand, within RR42_RS01235 at 279.859 kb on - strand, within RR42_RS01235 at 279.859 kb on - strand, within RR42_RS01235 at 279.859 kb on - strand, within RR42_RS01235 at 279.862 kb on + strand, within RR42_RS01235 at 279.862 kb on + strand, within RR42_RS01235 at 279.863 kb on - strand, within RR42_RS01235 at 279.863 kb on - strand, within RR42_RS01235 at 279.863 kb on - strand, within RR42_RS01235 at 279.892 kb on + strand, within RR42_RS01235 at 279.892 kb on + strand, within RR42_RS01235 at 279.893 kb on - strand, within RR42_RS01235 at 279.893 kb on - strand, within RR42_RS01235 at 279.893 kb on - strand, within RR42_RS01235 at 279.940 kb on + strand, within RR42_RS01235 at 279.940 kb on + strand, within RR42_RS01235 at 279.941 kb on - strand, within RR42_RS01235 at 280.004 kb on - strand, within RR42_RS01235 at 280.004 kb on - strand, within RR42_RS01235 at 280.231 kb on - strand at 280.249 kb on + strand at 280.249 kb on + strand at 280.249 kb on + strand at 280.250 kb on - strand at 280.250 kb on - strand at 280.259 kb on - strand at 280.417 kb on + strand at 280.418 kb on - strand at 280.418 kb on - strand at 280.418 kb on - strand at 280.441 kb on + strand at 280.442 kb on - strand at 280.593 kb on + strand, within RR42_RS01240 at 280.593 kb on + strand, within RR42_RS01240 at 280.593 kb on + strand, within RR42_RS01240 at 280.641 kb on + strand, within RR42_RS01240 at 280.656 kb on + strand, within RR42_RS01240 at 280.688 kb on + strand, within RR42_RS01240 at 280.689 kb on - strand, within RR42_RS01240 at 280.788 kb on + strand, within RR42_RS01240 at 280.789 kb on - strand, within RR42_RS01240 at 280.789 kb on - strand, within RR42_RS01240 at 280.827 kb on + strand, within RR42_RS01240 at 280.992 kb on + strand, within RR42_RS01240 at 280.992 kb on + strand, within RR42_RS01240 at 280.995 kb on + strand, within RR42_RS01240 at 280.995 kb on + strand, within RR42_RS01240 at 281.000 kb on + strand, within RR42_RS01240 at 281.000 kb on + strand, within RR42_RS01240 at 281.000 kb on + strand, within RR42_RS01240 at 281.000 kb on + strand, within RR42_RS01240 at 281.000 kb on + strand, within RR42_RS01240 at 281.119 kb on - strand, within RR42_RS01240 at 281.218 kb on - strand, within RR42_RS01240
Per-strain Table
Position Strand Gene LocusTag Fraction Carbon source 2-Deoxyadenosine 5-monophosphate 5 mM remove 278,696 - +0.4 278,831 + RR42_RS01230 0.24 -0.4 278,832 - RR42_RS01230 0.24 +0.6 278,873 + RR42_RS01230 0.30 +0.7 278,874 - RR42_RS01230 0.30 +0.1 278,874 - RR42_RS01230 0.30 -0.2 278,906 + RR42_RS01230 0.34 -0.8 278,906 + RR42_RS01230 0.34 +0.6 278,927 + RR42_RS01230 0.37 -1.6 278,927 + RR42_RS01230 0.37 -1.2 278,927 + RR42_RS01230 0.37 -0.1 278,928 - RR42_RS01230 0.37 +0.8 278,928 - RR42_RS01230 0.37 +0.0 278,942 + RR42_RS01230 0.39 -0.7 278,943 - RR42_RS01230 0.39 -0.0 278,983 - RR42_RS01230 0.45 +1.6 278,983 - RR42_RS01230 0.45 -0.4 279,034 + RR42_RS01230 0.52 +0.0 279,034 + RR42_RS01230 0.52 -1.2 279,056 + RR42_RS01230 0.55 -1.0 279,092 + RR42_RS01230 0.60 +1.2 279,092 + RR42_RS01230 0.60 +0.9 279,152 + RR42_RS01230 0.68 -0.1 279,153 - RR42_RS01230 0.68 +0.5 279,212 + RR42_RS01230 0.76 -0.1 279,212 + RR42_RS01230 0.76 -0.4 279,212 + RR42_RS01230 0.76 +0.8 279,213 - RR42_RS01230 0.76 -2.2 279,213 - RR42_RS01230 0.76 +0.3 279,335 + -0.1 279,336 - -0.3 279,355 - +0.3 279,380 + +1.1 279,778 + RR42_RS01235 0.38 -1.0 279,858 + RR42_RS01235 0.49 +1.2 279,858 + RR42_RS01235 0.49 -2.0 279,858 + RR42_RS01235 0.49 +0.7 279,858 + RR42_RS01235 0.49 +1.6 279,858 + RR42_RS01235 0.49 +0.1 279,859 - RR42_RS01235 0.49 +0.3 279,859 - RR42_RS01235 0.49 +0.3 279,859 - RR42_RS01235 0.49 +0.7 279,859 - RR42_RS01235 0.49 -1.5 279,859 - RR42_RS01235 0.49 -0.7 279,859 - RR42_RS01235 0.49 +1.8 279,859 - RR42_RS01235 0.49 +1.0 279,859 - RR42_RS01235 0.49 -0.9 279,859 - RR42_RS01235 0.49 +1.5 279,862 + RR42_RS01235 0.49 +1.5 279,862 + RR42_RS01235 0.49 +1.3 279,863 - RR42_RS01235 0.49 -3.7 279,863 - RR42_RS01235 0.49 +1.3 279,863 - RR42_RS01235 0.49 +0.3 279,892 + RR42_RS01235 0.53 +0.5 279,892 + RR42_RS01235 0.53 +1.1 279,893 - RR42_RS01235 0.53 -0.3 279,893 - RR42_RS01235 0.53 +0.9 279,893 - RR42_RS01235 0.53 -1.5 279,940 + RR42_RS01235 0.59 +0.8 279,940 + RR42_RS01235 0.59 -0.3 279,941 - RR42_RS01235 0.59 +0.6 280,004 - RR42_RS01235 0.67 -0.2 280,004 - RR42_RS01235 0.67 +1.2 280,231 - -0.9 280,249 + +0.3 280,249 + -1.0 280,249 + +0.8 280,250 - +1.3 280,250 - -0.2 280,259 - +1.2 280,417 + +0.8 280,418 - -0.1 280,418 - +0.5 280,418 - -1.1 280,441 + +0.3 280,442 - -0.2 280,593 + RR42_RS01240 0.16 +0.1 280,593 + RR42_RS01240 0.16 -0.5 280,593 + RR42_RS01240 0.16 -0.9 280,641 + RR42_RS01240 0.19 +0.3 280,656 + RR42_RS01240 0.20 +1.5 280,688 + RR42_RS01240 0.23 +0.1 280,689 - RR42_RS01240 0.23 +0.1 280,788 + RR42_RS01240 0.30 +0.9 280,789 - RR42_RS01240 0.30 +0.2 280,789 - RR42_RS01240 0.30 +0.4 280,827 + RR42_RS01240 0.33 +1.0 280,992 + RR42_RS01240 0.45 +1.0 280,992 + RR42_RS01240 0.45 +0.3 280,995 + RR42_RS01240 0.45 +1.0 280,995 + RR42_RS01240 0.45 +0.7 281,000 + RR42_RS01240 0.46 -1.6 281,000 + RR42_RS01240 0.46 -0.3 281,000 + RR42_RS01240 0.46 +0.2 281,000 + RR42_RS01240 0.46 +0.2 281,000 + RR42_RS01240 0.46 +1.6 281,119 - RR42_RS01240 0.54 +1.1 281,218 - RR42_RS01240 0.62 -1.9
Or see this region's nucleotide sequence