Experiment: Vogels_fungal_media; pH 5.5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS08645 and HSERO_RS08650 are separated by 546 nucleotides HSERO_RS08650 and HSERO_RS08655 are separated by 1 nucleotides HSERO_RS08655 and HSERO_RS08660 are separated by 76 nucleotides
HSERO_RS08645: HSERO_RS08645 - RNA polymerase sigma70 factor, at 1,962,512 to 1,963,090
_RS08645
HSERO_RS08650: HSERO_RS08650 - acetolactate synthase, at 1,963,637 to 1,965,355
_RS08650
HSERO_RS08655: HSERO_RS08655 - acetolactate synthase, at 1,965,357 to 1,965,848
_RS08655
HSERO_RS08660: HSERO_RS08660 - ketol-acid reductoisomerase, at 1,965,925 to 1,966,941
_RS08660
Position (kb)
1963
1964
1965
1966 Strain fitness (log2 ratio)
-7
-6
-5
-4
-3
-2
-1
0
1
2
3 at 1962.711 kb on - strand, within HSERO_RS08645 at 1962.711 kb on - strand, within HSERO_RS08645 at 1962.759 kb on - strand, within HSERO_RS08645 at 1962.787 kb on + strand, within HSERO_RS08645 at 1962.865 kb on + strand, within HSERO_RS08645 at 1963.030 kb on + strand, within HSERO_RS08645 at 1963.091 kb on + strand at 1963.118 kb on + strand at 1963.260 kb on + strand at 1963.376 kb on - strand at 1963.428 kb on - strand at 1963.428 kb on - strand at 1963.428 kb on - strand at 1963.441 kb on - strand at 1963.509 kb on + strand at 1963.833 kb on + strand, within HSERO_RS08650 at 1963.987 kb on + strand, within HSERO_RS08650 at 1963.995 kb on - strand, within HSERO_RS08650 at 1964.043 kb on - strand, within HSERO_RS08650 at 1964.110 kb on + strand, within HSERO_RS08650 at 1964.224 kb on - strand, within HSERO_RS08650 at 1964.357 kb on - strand, within HSERO_RS08650 at 1964.513 kb on - strand, within HSERO_RS08650 at 1964.616 kb on - strand, within HSERO_RS08650 at 1964.851 kb on + strand, within HSERO_RS08650 at 1964.998 kb on - strand, within HSERO_RS08650 at 1965.003 kb on - strand, within HSERO_RS08650 at 1965.289 kb on + strand at 1965.297 kb on - strand at 1965.457 kb on + strand, within HSERO_RS08655 at 1965.465 kb on - strand, within HSERO_RS08655 at 1965.489 kb on + strand, within HSERO_RS08655 at 1965.877 kb on + strand at 1965.903 kb on + strand at 1965.911 kb on - strand at 1966.060 kb on - strand, within HSERO_RS08660 at 1966.060 kb on - strand, within HSERO_RS08660
Per-strain Table
Position Strand Gene LocusTag Fraction Vogels_fungal_media; pH 5.5 remove 1,962,711 - HSERO_RS08645 0.34 -0.5 1,962,711 - HSERO_RS08645 0.34 +0.0 1,962,759 - HSERO_RS08645 0.43 +1.1 1,962,787 + HSERO_RS08645 0.47 +0.3 1,962,865 + HSERO_RS08645 0.61 +1.3 1,963,030 + HSERO_RS08645 0.89 +0.9 1,963,091 + -2.1 1,963,118 + +0.5 1,963,260 + +2.8 1,963,376 - -2.5 1,963,428 - -3.2 1,963,428 - -1.6 1,963,428 - -2.6 1,963,441 - -2.9 1,963,509 + -1.3 1,963,833 + HSERO_RS08650 0.11 -4.3 1,963,987 + HSERO_RS08650 0.20 -4.9 1,963,995 - HSERO_RS08650 0.21 -2.3 1,964,043 - HSERO_RS08650 0.24 -4.5 1,964,110 + HSERO_RS08650 0.28 -6.1 1,964,224 - HSERO_RS08650 0.34 -4.8 1,964,357 - HSERO_RS08650 0.42 -6.2 1,964,513 - HSERO_RS08650 0.51 -5.9 1,964,616 - HSERO_RS08650 0.57 -1.5 1,964,851 + HSERO_RS08650 0.71 -6.8 1,964,998 - HSERO_RS08650 0.79 -3.0 1,965,003 - HSERO_RS08650 0.79 -4.8 1,965,289 + -6.1 1,965,297 - -3.4 1,965,457 + HSERO_RS08655 0.20 -0.5 1,965,465 - HSERO_RS08655 0.22 -3.7 1,965,489 + HSERO_RS08655 0.27 -2.1 1,965,877 + -0.9 1,965,903 + -2.4 1,965,911 - -4.0 1,966,060 - HSERO_RS08660 0.13 -1.8 1,966,060 - HSERO_RS08660 0.13 -2.5
Or see this region's nucleotide sequence