Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS24915

Experiment: Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS24905 and BWI76_RS24910 are separated by 4 nucleotidesBWI76_RS24910 and BWI76_RS24915 are separated by 63 nucleotidesBWI76_RS24915 and BWI76_RS24920 overlap by 43 nucleotidesBWI76_RS24920 and BWI76_RS24925 are separated by 22 nucleotidesBWI76_RS24925 and BWI76_RS24930 are separated by 9 nucleotides BWI76_RS24905: BWI76_RS24905 - filamentation induced by cAMP protein fic, at 5,189,505 to 5,190,506 _RS24905 BWI76_RS24910: BWI76_RS24910 - rRNA (cytidine-2'-O-)-methyltransferase, at 5,190,511 to 5,191,374 _RS24910 BWI76_RS24915: BWI76_RS24915 - penicillin-binding protein activator, at 5,191,438 to 5,193,519 _RS24915 BWI76_RS24920: BWI76_RS24920 - YraN family protein, at 5,193,477 to 5,193,863 _RS24920 BWI76_RS24925: BWI76_RS24925 - DnaA initiator-associating protein DiaA, at 5,193,886 to 5,194,476 _RS24925 BWI76_RS24930: BWI76_RS24930 - osmotically-inducible protein OsmY, at 5,194,486 to 5,195,061 _RS24930 Position (kb) 5191 5192 5193 5194Strain fitness (log2 ratio) -2 -1 0 1 2 3at 5190.468 kb on + strandat 5190.481 kb on - strandat 5190.508 kb on + strandat 5190.517 kb on - strandat 5190.523 kb on - strandat 5190.536 kb on + strandat 5190.536 kb on + strandat 5190.536 kb on + strandat 5190.544 kb on - strandat 5190.544 kb on - strandat 5190.699 kb on - strand, within BWI76_RS24910at 5190.911 kb on - strand, within BWI76_RS24910at 5191.009 kb on + strand, within BWI76_RS24910at 5191.010 kb on + strand, within BWI76_RS24910at 5191.125 kb on - strand, within BWI76_RS24910at 5191.125 kb on - strand, within BWI76_RS24910at 5191.127 kb on + strand, within BWI76_RS24910at 5191.135 kb on - strand, within BWI76_RS24910at 5191.275 kb on + strand, within BWI76_RS24910at 5191.283 kb on - strand, within BWI76_RS24910at 5191.319 kb on - strandat 5191.336 kb on - strandat 5191.336 kb on - strandat 5191.721 kb on - strand, within BWI76_RS24915at 5191.935 kb on - strand, within BWI76_RS24915at 5191.978 kb on + strand, within BWI76_RS24915at 5192.049 kb on - strand, within BWI76_RS24915at 5192.064 kb on - strand, within BWI76_RS24915at 5192.195 kb on - strand, within BWI76_RS24915at 5192.334 kb on + strand, within BWI76_RS24915at 5192.431 kb on + strand, within BWI76_RS24915at 5192.563 kb on - strand, within BWI76_RS24915at 5192.681 kb on - strand, within BWI76_RS24915at 5192.703 kb on + strand, within BWI76_RS24915at 5192.833 kb on + strand, within BWI76_RS24915at 5193.028 kb on + strand, within BWI76_RS24915at 5193.028 kb on + strand, within BWI76_RS24915at 5193.028 kb on + strand, within BWI76_RS24915at 5193.036 kb on - strand, within BWI76_RS24915at 5193.040 kb on + strand, within BWI76_RS24915at 5193.040 kb on + strand, within BWI76_RS24915at 5193.041 kb on - strand, within BWI76_RS24915at 5193.048 kb on - strand, within BWI76_RS24915at 5193.158 kb on - strand, within BWI76_RS24915at 5193.170 kb on + strand, within BWI76_RS24915at 5193.170 kb on + strand, within BWI76_RS24915at 5193.172 kb on + strand, within BWI76_RS24915at 5193.207 kb on - strand, within BWI76_RS24915at 5193.323 kb on + strandat 5193.408 kb on - strandat 5193.412 kb on + strandat 5193.445 kb on + strandat 5193.636 kb on + strand, within BWI76_RS24920at 5193.745 kb on - strand, within BWI76_RS24920at 5193.807 kb on + strand, within BWI76_RS24920at 5194.007 kb on - strand, within BWI76_RS24925at 5194.186 kb on + strand, within BWI76_RS24925at 5194.314 kb on - strand, within BWI76_RS24925at 5194.493 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM
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5,190,468 + -0.8
5,190,481 - +0.3
5,190,508 + -0.2
5,190,517 - +0.1
5,190,523 - -0.3
5,190,536 + -0.6
5,190,536 + -0.8
5,190,536 + -0.0
5,190,544 - -0.6
5,190,544 - -1.0
5,190,699 - BWI76_RS24910 0.22 -0.1
5,190,911 - BWI76_RS24910 0.46 +0.1
5,191,009 + BWI76_RS24910 0.58 -1.0
5,191,010 + BWI76_RS24910 0.58 +0.7
5,191,125 - BWI76_RS24910 0.71 -1.3
5,191,125 - BWI76_RS24910 0.71 -1.0
5,191,127 + BWI76_RS24910 0.71 -1.8
5,191,135 - BWI76_RS24910 0.72 -0.8
5,191,275 + BWI76_RS24910 0.88 -1.8
5,191,283 - BWI76_RS24910 0.89 +0.2
5,191,319 - -1.0
5,191,336 - -0.2
5,191,336 - +1.1
5,191,721 - BWI76_RS24915 0.14 +1.3
5,191,935 - BWI76_RS24915 0.24 +0.9
5,191,978 + BWI76_RS24915 0.26 +1.3
5,192,049 - BWI76_RS24915 0.29 +0.4
5,192,064 - BWI76_RS24915 0.30 +0.8
5,192,195 - BWI76_RS24915 0.36 +1.4
5,192,334 + BWI76_RS24915 0.43 -0.6
5,192,431 + BWI76_RS24915 0.48 +0.9
5,192,563 - BWI76_RS24915 0.54 +1.3
5,192,681 - BWI76_RS24915 0.60 +0.9
5,192,703 + BWI76_RS24915 0.61 +1.5
5,192,833 + BWI76_RS24915 0.67 +0.8
5,193,028 + BWI76_RS24915 0.76 +1.3
5,193,028 + BWI76_RS24915 0.76 +0.8
5,193,028 + BWI76_RS24915 0.76 +3.2
5,193,036 - BWI76_RS24915 0.77 +1.1
5,193,040 + BWI76_RS24915 0.77 +1.2
5,193,040 + BWI76_RS24915 0.77 +1.2
5,193,041 - BWI76_RS24915 0.77 +0.7
5,193,048 - BWI76_RS24915 0.77 +0.8
5,193,158 - BWI76_RS24915 0.83 +0.7
5,193,170 + BWI76_RS24915 0.83 +1.0
5,193,170 + BWI76_RS24915 0.83 +2.0
5,193,172 + BWI76_RS24915 0.83 +1.1
5,193,207 - BWI76_RS24915 0.85 +1.1
5,193,323 + +0.6
5,193,408 - +2.8
5,193,412 + +0.0
5,193,445 + +0.9
5,193,636 + BWI76_RS24920 0.41 -0.4
5,193,745 - BWI76_RS24920 0.69 -0.4
5,193,807 + BWI76_RS24920 0.85 -0.6
5,194,007 - BWI76_RS24925 0.20 -0.1
5,194,186 + BWI76_RS24925 0.51 -0.7
5,194,314 - BWI76_RS24925 0.72 +0.2
5,194,493 - -0.4

Or see this region's nucleotide sequence