Experiment: Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16385 and BWI76_RS16390 are separated by 596 nucleotides BWI76_RS16390 and BWI76_RS16395 are separated by 108 nucleotides BWI76_RS16395 and BWI76_RS16400 are separated by 47 nucleotides
BWI76_RS16385: BWI76_RS16385 - endonuclease III, at 3,399,202 to 3,399,837
_RS16385
BWI76_RS16390: BWI76_RS16390 - dipeptide/tripeptide permease A, at 3,400,434 to 3,401,939
_RS16390
BWI76_RS16395: BWI76_RS16395 - glutathione S-transferase, at 3,402,048 to 3,402,653
_RS16395
BWI76_RS16400: BWI76_RS16400 - pyridoxal kinase, at 3,402,701 to 3,403,561
_RS16400
Position (kb)
3400
3401
3402 Strain fitness (log2 ratio)
-1
0
1 at 3399.711 kb on + strand, within BWI76_RS16385 at 3400.267 kb on - strand at 3400.308 kb on - strand at 3400.448 kb on + strand at 3400.488 kb on - strand at 3400.817 kb on + strand, within BWI76_RS16390 at 3400.817 kb on + strand, within BWI76_RS16390 at 3400.825 kb on - strand, within BWI76_RS16390 at 3401.195 kb on + strand, within BWI76_RS16390 at 3401.301 kb on + strand, within BWI76_RS16390 at 3401.391 kb on - strand, within BWI76_RS16390 at 3402.033 kb on + strand at 3402.111 kb on + strand, within BWI76_RS16395 at 3402.119 kb on - strand, within BWI76_RS16395 at 3402.407 kb on + strand, within BWI76_RS16395 at 3402.565 kb on - strand, within BWI76_RS16395 at 3402.727 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM remove 3,399,711 + BWI76_RS16385 0.80 +0.0 3,400,267 - -0.8 3,400,308 - -0.9 3,400,448 + +0.6 3,400,488 - -0.5 3,400,817 + BWI76_RS16390 0.25 -1.5 3,400,817 + BWI76_RS16390 0.25 -0.7 3,400,825 - BWI76_RS16390 0.26 -0.5 3,401,195 + BWI76_RS16390 0.51 -0.7 3,401,301 + BWI76_RS16390 0.58 -1.4 3,401,391 - BWI76_RS16390 0.64 -0.2 3,402,033 + -0.2 3,402,111 + BWI76_RS16395 0.10 -0.5 3,402,119 - BWI76_RS16395 0.12 +0.2 3,402,407 + BWI76_RS16395 0.59 +0.6 3,402,565 - BWI76_RS16395 0.85 +0.4 3,402,727 + +0.1
Or see this region's nucleotide sequence