Experiment: Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS11320 and BWI76_RS11325 are separated by 20 nucleotides BWI76_RS11325 and BWI76_RS11330 are separated by 236 nucleotides BWI76_RS11330 and BWI76_RS11335 are separated by 156 nucleotides BWI76_RS11335 and BWI76_RS11340 are separated by 5 nucleotides BWI76_RS11340 and BWI76_RS11345 are separated by 100 nucleotides
BWI76_RS11320: BWI76_RS11320 - NUDIX hydrolase, at 2,386,433 to 2,386,909
_RS11320
BWI76_RS11325: BWI76_RS11325 - 23S rRNA pseudouridine synthase E, at 2,386,930 to 2,387,580
_RS11325
BWI76_RS11330: BWI76_RS11330 - isocitrate dehydrogenase (NADP(+)), at 2,387,817 to 2,389,067
_RS11330
BWI76_RS11335: BWI76_RS11335 - tautomerase, at 2,389,224 to 2,389,610
_RS11335
BWI76_RS11340: BWI76_RS11340 - carboxymuconolactone decarboxylase, at 2,389,616 to 2,389,918
_RS11340
BWI76_RS11345: BWI76_RS11345 - LysR family transcriptional regulator, at 2,390,019 to 2,390,903
_RS11345
Position (kb)
2387
2388
2389
2390 Strain fitness (log2 ratio)
-2
-1
0
1 at 2387.180 kb on - strand, within BWI76_RS11325 at 2387.561 kb on + strand at 2387.608 kb on - strand at 2387.769 kb on + strand at 2389.183 kb on - strand at 2389.230 kb on - strand at 2389.232 kb on + strand at 2389.346 kb on + strand, within BWI76_RS11335 at 2389.581 kb on - strand at 2389.613 kb on + strand at 2389.748 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM remove 2,387,180 - BWI76_RS11325 0.38 -0.4 2,387,561 + +0.2 2,387,608 - +0.4 2,387,769 + +0.9 2,389,183 - +0.6 2,389,230 - +0.0 2,389,232 + -0.1 2,389,346 + BWI76_RS11335 0.32 -1.9 2,389,581 - +0.5 2,389,613 + -0.5 2,389,748 - -1.3
Or see this region's nucleotide sequence