Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS11330

Experiment: Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS11320 and BWI76_RS11325 are separated by 20 nucleotidesBWI76_RS11325 and BWI76_RS11330 are separated by 236 nucleotidesBWI76_RS11330 and BWI76_RS11335 are separated by 156 nucleotidesBWI76_RS11335 and BWI76_RS11340 are separated by 5 nucleotidesBWI76_RS11340 and BWI76_RS11345 are separated by 100 nucleotides BWI76_RS11320: BWI76_RS11320 - NUDIX hydrolase, at 2,386,433 to 2,386,909 _RS11320 BWI76_RS11325: BWI76_RS11325 - 23S rRNA pseudouridine synthase E, at 2,386,930 to 2,387,580 _RS11325 BWI76_RS11330: BWI76_RS11330 - isocitrate dehydrogenase (NADP(+)), at 2,387,817 to 2,389,067 _RS11330 BWI76_RS11335: BWI76_RS11335 - tautomerase, at 2,389,224 to 2,389,610 _RS11335 BWI76_RS11340: BWI76_RS11340 - carboxymuconolactone decarboxylase, at 2,389,616 to 2,389,918 _RS11340 BWI76_RS11345: BWI76_RS11345 - LysR family transcriptional regulator, at 2,390,019 to 2,390,903 _RS11345 Position (kb) 2387 2388 2389 2390Strain fitness (log2 ratio) -2 -1 0 1at 2387.180 kb on - strand, within BWI76_RS11325at 2387.561 kb on + strandat 2387.608 kb on - strandat 2387.769 kb on + strandat 2389.183 kb on - strandat 2389.230 kb on - strandat 2389.232 kb on + strandat 2389.346 kb on + strand, within BWI76_RS11335at 2389.581 kb on - strandat 2389.613 kb on + strandat 2389.748 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM
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2,387,180 - BWI76_RS11325 0.38 -0.4
2,387,561 + +0.2
2,387,608 - +0.4
2,387,769 + +0.9
2,389,183 - +0.6
2,389,230 - +0.0
2,389,232 + -0.1
2,389,346 + BWI76_RS11335 0.32 -1.9
2,389,581 - +0.5
2,389,613 + -0.5
2,389,748 - -1.3

Or see this region's nucleotide sequence