Experiment: MRS + carbenicillin Time8hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS18310 and BBR_RS18315 are separated by 31 nucleotides BBR_RS18315 and BBR_RS18320 are separated by 174 nucleotides BBR_RS18320 and BBR_RS18325 are separated by 303 nucleotides
BBR_RS18310: BBR_RS18310 - linear amide C-N hydrolase, at 1,896,554 to 1,897,555
_RS18310
BBR_RS18315: BBR_RS18315 - hypothetical protein, at 1,897,587 to 1,898,126
_RS18315
BBR_RS18320: BBR_RS18320 - hypothetical protein, at 1,898,301 to 1,898,726
_RS18320
BBR_RS18325: BBR_RS18325 - DNA-binding protein, at 1,899,030 to 1,899,476
_RS18325
Position (kb)
1897
1898
1899 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 1896.620 kb on - strand at 1896.625 kb on + strand at 1896.625 kb on + strand at 1896.625 kb on + strand at 1896.631 kb on - strand at 1896.633 kb on - strand at 1896.633 kb on - strand at 1896.646 kb on - strand at 1896.648 kb on + strand at 1896.648 kb on + strand at 1896.648 kb on + strand at 1896.648 kb on + strand at 1896.654 kb on + strand at 1896.688 kb on - strand, within BBR_RS18310 at 1896.725 kb on + strand, within BBR_RS18310 at 1896.748 kb on + strand, within BBR_RS18310 at 1896.771 kb on - strand, within BBR_RS18310 at 1896.774 kb on + strand, within BBR_RS18310 at 1896.774 kb on + strand, within BBR_RS18310 at 1896.793 kb on + strand, within BBR_RS18310 at 1896.796 kb on - strand, within BBR_RS18310 at 1896.803 kb on + strand, within BBR_RS18310 at 1896.803 kb on + strand, within BBR_RS18310 at 1896.811 kb on - strand, within BBR_RS18310 at 1896.811 kb on - strand, within BBR_RS18310 at 1896.811 kb on - strand, within BBR_RS18310 at 1896.811 kb on - strand, within BBR_RS18310 at 1896.837 kb on - strand, within BBR_RS18310 at 1896.856 kb on + strand, within BBR_RS18310 at 1896.864 kb on - strand, within BBR_RS18310 at 1896.866 kb on + strand, within BBR_RS18310 at 1896.877 kb on + strand, within BBR_RS18310 at 1896.884 kb on + strand, within BBR_RS18310 at 1896.887 kb on - strand, within BBR_RS18310 at 1896.889 kb on + strand, within BBR_RS18310 at 1896.889 kb on + strand, within BBR_RS18310 at 1896.897 kb on - strand, within BBR_RS18310 at 1896.897 kb on - strand, within BBR_RS18310 at 1896.897 kb on - strand, within BBR_RS18310 at 1896.914 kb on + strand, within BBR_RS18310 at 1896.933 kb on + strand, within BBR_RS18310 at 1896.934 kb on + strand, within BBR_RS18310 at 1896.957 kb on + strand, within BBR_RS18310 at 1896.971 kb on - strand, within BBR_RS18310 at 1896.971 kb on - strand, within BBR_RS18310 at 1896.979 kb on + strand, within BBR_RS18310 at 1896.979 kb on + strand, within BBR_RS18310 at 1896.993 kb on - strand, within BBR_RS18310 at 1897.009 kb on + strand, within BBR_RS18310 at 1897.028 kb on + strand, within BBR_RS18310 at 1897.039 kb on + strand, within BBR_RS18310 at 1897.039 kb on + strand, within BBR_RS18310 at 1897.045 kb on + strand, within BBR_RS18310 at 1897.046 kb on + strand, within BBR_RS18310 at 1897.046 kb on + strand, within BBR_RS18310 at 1897.057 kb on + strand, within BBR_RS18310 at 1897.080 kb on - strand, within BBR_RS18310 at 1897.085 kb on + strand, within BBR_RS18310 at 1897.100 kb on - strand, within BBR_RS18310 at 1897.105 kb on - strand, within BBR_RS18310 at 1897.105 kb on - strand, within BBR_RS18310 at 1897.126 kb on - strand, within BBR_RS18310 at 1897.128 kb on + strand, within BBR_RS18310 at 1897.128 kb on + strand, within BBR_RS18310 at 1897.128 kb on + strand, within BBR_RS18310 at 1897.128 kb on + strand, within BBR_RS18310 at 1897.131 kb on - strand, within BBR_RS18310 at 1897.136 kb on - strand, within BBR_RS18310 at 1897.136 kb on - strand, within BBR_RS18310 at 1897.138 kb on + strand, within BBR_RS18310 at 1897.138 kb on + strand, within BBR_RS18310 at 1897.156 kb on + strand, within BBR_RS18310 at 1897.156 kb on - strand, within BBR_RS18310 at 1897.166 kb on + strand, within BBR_RS18310 at 1897.179 kb on - strand, within BBR_RS18310 at 1897.184 kb on + strand, within BBR_RS18310 at 1897.187 kb on - strand, within BBR_RS18310 at 1897.200 kb on + strand, within BBR_RS18310 at 1897.208 kb on - strand, within BBR_RS18310 at 1897.208 kb on - strand, within BBR_RS18310 at 1897.208 kb on - strand, within BBR_RS18310 at 1897.208 kb on - strand, within BBR_RS18310 at 1897.237 kb on - strand, within BBR_RS18310 at 1897.237 kb on - strand, within BBR_RS18310 at 1897.237 kb on - strand, within BBR_RS18310 at 1897.237 kb on - strand, within BBR_RS18310 at 1897.237 kb on - strand, within BBR_RS18310 at 1897.244 kb on + strand, within BBR_RS18310 at 1897.261 kb on + strand, within BBR_RS18310 at 1897.264 kb on + strand, within BBR_RS18310 at 1897.269 kb on - strand, within BBR_RS18310 at 1897.269 kb on - strand, within BBR_RS18310 at 1897.269 kb on - strand, within BBR_RS18310 at 1897.271 kb on + strand, within BBR_RS18310 at 1897.309 kb on + strand, within BBR_RS18310 at 1897.309 kb on + strand, within BBR_RS18310 at 1897.317 kb on - strand, within BBR_RS18310 at 1897.326 kb on + strand, within BBR_RS18310 at 1897.334 kb on - strand, within BBR_RS18310 at 1897.364 kb on - strand, within BBR_RS18310 at 1897.364 kb on - strand, within BBR_RS18310 at 1897.388 kb on + strand, within BBR_RS18310 at 1897.406 kb on - strand, within BBR_RS18310 at 1897.408 kb on - strand, within BBR_RS18310 at 1897.432 kb on - strand, within BBR_RS18310 at 1897.434 kb on - strand, within BBR_RS18310 at 1897.434 kb on - strand, within BBR_RS18310 at 1897.455 kb on + strand at 1897.456 kb on + strand at 1897.457 kb on - strand at 1897.457 kb on - strand at 1897.502 kb on - strand at 1897.504 kb on + strand at 1897.513 kb on + strand at 1897.513 kb on + strand at 1897.717 kb on + strand, within BBR_RS18315 at 1897.778 kb on + strand, within BBR_RS18315 at 1897.785 kb on + strand, within BBR_RS18315 at 1897.790 kb on + strand, within BBR_RS18315 at 1897.792 kb on - strand, within BBR_RS18315 at 1897.952 kb on + strand, within BBR_RS18315 at 1897.955 kb on - strand, within BBR_RS18315 at 1897.970 kb on - strand, within BBR_RS18315 at 1897.975 kb on - strand, within BBR_RS18315 at 1897.979 kb on + strand, within BBR_RS18315 at 1897.999 kb on + strand, within BBR_RS18315 at 1898.043 kb on - strand, within BBR_RS18315 at 1898.108 kb on + strand at 1898.116 kb on - strand at 1898.122 kb on + strand at 1898.129 kb on + strand at 1898.129 kb on + strand at 1898.130 kb on - strand at 1898.151 kb on - strand at 1898.157 kb on - strand at 1898.167 kb on + strand at 1898.167 kb on + strand at 1898.177 kb on + strand at 1898.203 kb on + strand at 1898.211 kb on - strand at 1898.211 kb on - strand at 1898.213 kb on - strand at 1898.220 kb on + strand at 1898.228 kb on - strand at 1898.228 kb on - strand at 1898.230 kb on - strand at 1898.259 kb on - strand at 1898.350 kb on + strand, within BBR_RS18320 at 1898.351 kb on + strand, within BBR_RS18320 at 1898.359 kb on + strand, within BBR_RS18320 at 1898.418 kb on - strand, within BBR_RS18320 at 1898.455 kb on + strand, within BBR_RS18320 at 1898.479 kb on + strand, within BBR_RS18320 at 1898.488 kb on - strand, within BBR_RS18320 at 1898.545 kb on + strand, within BBR_RS18320 at 1898.545 kb on + strand, within BBR_RS18320 at 1898.545 kb on + strand, within BBR_RS18320 at 1898.553 kb on - strand, within BBR_RS18320 at 1898.576 kb on - strand, within BBR_RS18320 at 1898.600 kb on - strand, within BBR_RS18320 at 1898.666 kb on - strand, within BBR_RS18320 at 1898.675 kb on + strand, within BBR_RS18320 at 1898.683 kb on - strand, within BBR_RS18320 at 1898.683 kb on - strand, within BBR_RS18320 at 1898.708 kb on + strand at 1898.708 kb on + strand at 1898.708 kb on + strand at 1898.708 kb on + strand at 1898.742 kb on + strand at 1898.742 kb on + strand at 1898.750 kb on - strand at 1898.780 kb on + strand at 1898.783 kb on - strand at 1898.855 kb on + strand at 1898.862 kb on + strand at 1898.862 kb on + strand at 1898.863 kb on - strand at 1898.863 kb on - strand at 1898.863 kb on - strand at 1898.912 kb on + strand at 1898.947 kb on + strand at 1898.967 kb on - strand at 1899.046 kb on + strand at 1899.048 kb on - strand at 1899.054 kb on - strand at 1899.065 kb on + strand at 1899.067 kb on + strand at 1899.067 kb on + strand at 1899.074 kb on + strand at 1899.108 kb on + strand, within BBR_RS18325 at 1899.111 kb on + strand, within BBR_RS18325
Per-strain Table
Position Strand Gene LocusTag Fraction MRS + carbenicillin Time8hr remove 1,896,620 - -1.5 1,896,625 + -0.7 1,896,625 + -0.3 1,896,625 + +1.9 1,896,631 - +2.2 1,896,633 - -1.4 1,896,633 - -0.5 1,896,646 - -1.5 1,896,648 + +0.1 1,896,648 + +0.2 1,896,648 + +0.5 1,896,648 + -0.1 1,896,654 + +1.7 1,896,688 - BBR_RS18310 0.13 +0.1 1,896,725 + BBR_RS18310 0.17 -3.1 1,896,748 + BBR_RS18310 0.19 -0.3 1,896,771 - BBR_RS18310 0.22 -1.5 1,896,774 + BBR_RS18310 0.22 -1.3 1,896,774 + BBR_RS18310 0.22 +1.7 1,896,793 + BBR_RS18310 0.24 -0.7 1,896,796 - BBR_RS18310 0.24 +0.2 1,896,803 + BBR_RS18310 0.25 -1.2 1,896,803 + BBR_RS18310 0.25 -1.1 1,896,811 - BBR_RS18310 0.26 +0.5 1,896,811 - BBR_RS18310 0.26 +1.1 1,896,811 - BBR_RS18310 0.26 -1.1 1,896,811 - BBR_RS18310 0.26 +2.3 1,896,837 - BBR_RS18310 0.28 -0.0 1,896,856 + BBR_RS18310 0.30 +0.4 1,896,864 - BBR_RS18310 0.31 -0.6 1,896,866 + BBR_RS18310 0.31 -0.4 1,896,877 + BBR_RS18310 0.32 -0.7 1,896,884 + BBR_RS18310 0.33 -1.5 1,896,887 - BBR_RS18310 0.33 -0.6 1,896,889 + BBR_RS18310 0.33 -0.9 1,896,889 + BBR_RS18310 0.33 -0.7 1,896,897 - BBR_RS18310 0.34 +2.4 1,896,897 - BBR_RS18310 0.34 +0.2 1,896,897 - BBR_RS18310 0.34 -0.9 1,896,914 + BBR_RS18310 0.36 +3.2 1,896,933 + BBR_RS18310 0.38 -0.6 1,896,934 + BBR_RS18310 0.38 -1.5 1,896,957 + BBR_RS18310 0.40 -0.5 1,896,971 - BBR_RS18310 0.42 +0.1 1,896,971 - BBR_RS18310 0.42 +3.1 1,896,979 + BBR_RS18310 0.42 +2.8 1,896,979 + BBR_RS18310 0.42 +0.5 1,896,993 - BBR_RS18310 0.44 -1.5 1,897,009 + BBR_RS18310 0.45 -0.2 1,897,028 + BBR_RS18310 0.47 +0.1 1,897,039 + BBR_RS18310 0.48 +0.1 1,897,039 + BBR_RS18310 0.48 -1.5 1,897,045 + BBR_RS18310 0.49 +1.1 1,897,046 + BBR_RS18310 0.49 +0.1 1,897,046 + BBR_RS18310 0.49 +0.9 1,897,057 + BBR_RS18310 0.50 +1.0 1,897,080 - BBR_RS18310 0.52 +0.7 1,897,085 + BBR_RS18310 0.53 +0.1 1,897,100 - BBR_RS18310 0.54 -1.5 1,897,105 - BBR_RS18310 0.55 +1.5 1,897,105 - BBR_RS18310 0.55 -1.6 1,897,126 - BBR_RS18310 0.57 +1.3 1,897,128 + BBR_RS18310 0.57 -2.6 1,897,128 + BBR_RS18310 0.57 -0.1 1,897,128 + BBR_RS18310 0.57 +4.0 1,897,128 + BBR_RS18310 0.57 +0.3 1,897,131 - BBR_RS18310 0.58 -0.4 1,897,136 - BBR_RS18310 0.58 -0.5 1,897,136 - BBR_RS18310 0.58 +1.1 1,897,138 + BBR_RS18310 0.58 +0.8 1,897,138 + BBR_RS18310 0.58 -0.8 1,897,156 + BBR_RS18310 0.60 +0.3 1,897,156 - BBR_RS18310 0.60 -0.5 1,897,166 + BBR_RS18310 0.61 +0.1 1,897,179 - BBR_RS18310 0.62 +2.3 1,897,184 + BBR_RS18310 0.63 -1.8 1,897,187 - BBR_RS18310 0.63 -0.1 1,897,200 + BBR_RS18310 0.64 -0.4 1,897,208 - BBR_RS18310 0.65 -1.2 1,897,208 - BBR_RS18310 0.65 -0.5 1,897,208 - BBR_RS18310 0.65 +1.1 1,897,208 - BBR_RS18310 0.65 -0.3 1,897,237 - BBR_RS18310 0.68 +0.3 1,897,237 - BBR_RS18310 0.68 +1.3 1,897,237 - BBR_RS18310 0.68 -0.4 1,897,237 - BBR_RS18310 0.68 +3.1 1,897,237 - BBR_RS18310 0.68 -1.9 1,897,244 + BBR_RS18310 0.69 -0.0 1,897,261 + BBR_RS18310 0.71 -0.9 1,897,264 + BBR_RS18310 0.71 +2.5 1,897,269 - BBR_RS18310 0.71 +0.4 1,897,269 - BBR_RS18310 0.71 +0.1 1,897,269 - BBR_RS18310 0.71 +3.2 1,897,271 + BBR_RS18310 0.72 -0.7 1,897,309 + BBR_RS18310 0.75 +0.2 1,897,309 + BBR_RS18310 0.75 -0.3 1,897,317 - BBR_RS18310 0.76 +0.6 1,897,326 + BBR_RS18310 0.77 -1.5 1,897,334 - BBR_RS18310 0.78 -0.5 1,897,364 - BBR_RS18310 0.81 +0.3 1,897,364 - BBR_RS18310 0.81 -1.2 1,897,388 + BBR_RS18310 0.83 +1.5 1,897,406 - BBR_RS18310 0.85 +1.2 1,897,408 - BBR_RS18310 0.85 +2.3 1,897,432 - BBR_RS18310 0.88 -0.5 1,897,434 - BBR_RS18310 0.88 -0.9 1,897,434 - BBR_RS18310 0.88 -0.5 1,897,455 + -0.2 1,897,456 + +1.9 1,897,457 - -0.2 1,897,457 - +1.3 1,897,502 - -1.2 1,897,504 + +2.4 1,897,513 + -0.5 1,897,513 + -0.1 1,897,717 + BBR_RS18315 0.24 +0.4 1,897,778 + BBR_RS18315 0.35 +2.5 1,897,785 + BBR_RS18315 0.37 +1.8 1,897,790 + BBR_RS18315 0.38 +3.7 1,897,792 - BBR_RS18315 0.38 -0.7 1,897,952 + BBR_RS18315 0.68 +3.0 1,897,955 - BBR_RS18315 0.68 -3.5 1,897,970 - BBR_RS18315 0.71 +0.3 1,897,975 - BBR_RS18315 0.72 +0.7 1,897,979 + BBR_RS18315 0.73 +1.4 1,897,999 + BBR_RS18315 0.76 -0.0 1,898,043 - BBR_RS18315 0.84 +1.0 1,898,108 + -0.8 1,898,116 - +1.7 1,898,122 + +0.8 1,898,129 + +0.5 1,898,129 + +0.1 1,898,130 - -3.8 1,898,151 - +0.2 1,898,157 - +2.0 1,898,167 + +0.5 1,898,167 + +1.7 1,898,177 + +0.4 1,898,203 + +1.1 1,898,211 - +1.9 1,898,211 - +0.8 1,898,213 - -1.5 1,898,220 + -0.2 1,898,228 - -1.0 1,898,228 - -1.9 1,898,230 - -0.4 1,898,259 - +0.6 1,898,350 + BBR_RS18320 0.12 -1.5 1,898,351 + BBR_RS18320 0.12 +1.4 1,898,359 + BBR_RS18320 0.14 +2.5 1,898,418 - BBR_RS18320 0.27 -0.3 1,898,455 + BBR_RS18320 0.36 +1.3 1,898,479 + BBR_RS18320 0.42 -1.5 1,898,488 - BBR_RS18320 0.44 +0.9 1,898,545 + BBR_RS18320 0.57 +0.5 1,898,545 + BBR_RS18320 0.57 +1.1 1,898,545 + BBR_RS18320 0.57 +0.6 1,898,553 - BBR_RS18320 0.59 -0.3 1,898,576 - BBR_RS18320 0.65 -2.5 1,898,600 - BBR_RS18320 0.70 -0.4 1,898,666 - BBR_RS18320 0.86 +2.5 1,898,675 + BBR_RS18320 0.88 +1.3 1,898,683 - BBR_RS18320 0.90 -1.2 1,898,683 - BBR_RS18320 0.90 +0.3 1,898,708 + +0.1 1,898,708 + +0.1 1,898,708 + +0.9 1,898,708 + +0.7 1,898,742 + -0.8 1,898,742 + -3.5 1,898,750 - +2.7 1,898,780 + -0.4 1,898,783 - -2.4 1,898,855 + +1.4 1,898,862 + +1.3 1,898,862 + +0.1 1,898,863 - +0.0 1,898,863 - +1.5 1,898,863 - +0.1 1,898,912 + +2.3 1,898,947 + -0.7 1,898,967 - +0.3 1,899,046 + -1.5 1,899,048 - -0.1 1,899,054 - +0.5 1,899,065 + -1.1 1,899,067 + +0.1 1,899,067 + -0.6 1,899,074 + -0.6 1,899,108 + BBR_RS18325 0.17 +0.3 1,899,111 + BBR_RS18325 0.18 -1.0
Or see this region's nucleotide sequence