Experiment: 50% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_28350 and AO356_28355 are separated by 39 nucleotides AO356_28355 and AO356_28360 are separated by 115 nucleotides
AO356_28350: AO356_28350 - spermidine/putrescine ABC transporter substrate-binding protein, at 6,593,175 to 6,594,278
_28350
AO356_28355: AO356_28355 - hypothetical protein, at 6,594,318 to 6,595,655
_28355
AO356_28360: AO356_28360 - hydrolase, at 6,595,771 to 6,597,033
_28360
Position (kb)
6594
6595
6596 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 6593.395 kb on + strand, within AO356_28350 at 6593.410 kb on + strand, within AO356_28350 at 6593.496 kb on + strand, within AO356_28350 at 6593.620 kb on + strand, within AO356_28350 at 6593.629 kb on + strand, within AO356_28350 at 6593.668 kb on + strand, within AO356_28350 at 6593.668 kb on + strand, within AO356_28350 at 6593.668 kb on + strand, within AO356_28350 at 6593.669 kb on - strand, within AO356_28350 at 6593.669 kb on - strand, within AO356_28350 at 6593.707 kb on + strand, within AO356_28350 at 6593.707 kb on + strand, within AO356_28350 at 6593.708 kb on - strand, within AO356_28350 at 6593.835 kb on + strand, within AO356_28350 at 6593.836 kb on - strand, within AO356_28350 at 6593.844 kb on + strand, within AO356_28350 at 6593.887 kb on + strand, within AO356_28350 at 6593.888 kb on - strand, within AO356_28350 at 6593.888 kb on - strand, within AO356_28350 at 6593.888 kb on - strand, within AO356_28350 at 6594.112 kb on + strand, within AO356_28350 at 6594.184 kb on + strand at 6594.322 kb on + strand at 6594.322 kb on + strand at 6594.338 kb on - strand at 6594.338 kb on - strand at 6594.338 kb on - strand at 6594.411 kb on - strand at 6594.411 kb on - strand at 6594.412 kb on + strand at 6594.412 kb on + strand at 6594.413 kb on - strand at 6594.508 kb on + strand, within AO356_28355 at 6594.509 kb on - strand, within AO356_28355 at 6594.622 kb on + strand, within AO356_28355 at 6594.623 kb on - strand, within AO356_28355 at 6594.623 kb on - strand, within AO356_28355 at 6594.951 kb on - strand, within AO356_28355 at 6595.159 kb on + strand, within AO356_28355 at 6595.222 kb on + strand, within AO356_28355 at 6595.222 kb on + strand, within AO356_28355 at 6595.399 kb on + strand, within AO356_28355 at 6595.426 kb on + strand, within AO356_28355 at 6595.426 kb on + strand, within AO356_28355 at 6595.426 kb on + strand, within AO356_28355 at 6595.655 kb on + strand at 6595.656 kb on - strand at 6595.673 kb on + strand at 6595.674 kb on - strand at 6595.899 kb on - strand, within AO356_28360 at 6595.952 kb on + strand, within AO356_28360 at 6595.952 kb on + strand, within AO356_28360 at 6595.952 kb on + strand, within AO356_28360 at 6595.953 kb on - strand, within AO356_28360 at 6596.012 kb on + strand, within AO356_28360 at 6596.012 kb on + strand, within AO356_28360 at 6596.013 kb on - strand, within AO356_28360 at 6596.348 kb on + strand, within AO356_28360 at 6596.348 kb on + strand, within AO356_28360 at 6596.375 kb on + strand, within AO356_28360 at 6596.376 kb on - strand, within AO356_28360 at 6596.408 kb on + strand, within AO356_28360 at 6596.408 kb on + strand, within AO356_28360 at 6596.408 kb on + strand, within AO356_28360 at 6596.409 kb on - strand, within AO356_28360 at 6596.409 kb on - strand, within AO356_28360 at 6596.534 kb on + strand, within AO356_28360
Per-strain Table
Position Strand Gene LocusTag Fraction 50% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine remove 6,593,395 + AO356_28350 0.20 -0.7 6,593,410 + AO356_28350 0.21 -0.7 6,593,496 + AO356_28350 0.29 +0.4 6,593,620 + AO356_28350 0.40 +0.4 6,593,629 + AO356_28350 0.41 +1.0 6,593,668 + AO356_28350 0.45 -0.8 6,593,668 + AO356_28350 0.45 -0.9 6,593,668 + AO356_28350 0.45 +1.1 6,593,669 - AO356_28350 0.45 -0.2 6,593,669 - AO356_28350 0.45 +0.9 6,593,707 + AO356_28350 0.48 +1.7 6,593,707 + AO356_28350 0.48 -0.6 6,593,708 - AO356_28350 0.48 +0.5 6,593,835 + AO356_28350 0.60 +0.3 6,593,836 - AO356_28350 0.60 +1.4 6,593,844 + AO356_28350 0.61 -0.3 6,593,887 + AO356_28350 0.64 -0.6 6,593,888 - AO356_28350 0.65 +0.6 6,593,888 - AO356_28350 0.65 -0.8 6,593,888 - AO356_28350 0.65 +0.2 6,594,112 + AO356_28350 0.85 +0.2 6,594,184 + -0.3 6,594,322 + +0.1 6,594,322 + -0.3 6,594,338 - -0.3 6,594,338 - +0.5 6,594,338 - -0.1 6,594,411 - +0.1 6,594,411 - -0.6 6,594,412 + +1.0 6,594,412 + +0.3 6,594,413 - +0.4 6,594,508 + AO356_28355 0.14 -1.5 6,594,509 - AO356_28355 0.14 -0.3 6,594,622 + AO356_28355 0.23 +0.7 6,594,623 - AO356_28355 0.23 -0.8 6,594,623 - AO356_28355 0.23 +1.1 6,594,951 - AO356_28355 0.47 -0.1 6,595,159 + AO356_28355 0.63 +0.2 6,595,222 + AO356_28355 0.68 +2.5 6,595,222 + AO356_28355 0.68 -0.0 6,595,399 + AO356_28355 0.81 -0.8 6,595,426 + AO356_28355 0.83 -2.0 6,595,426 + AO356_28355 0.83 -0.6 6,595,426 + AO356_28355 0.83 -0.2 6,595,655 + -0.2 6,595,656 - -0.3 6,595,673 + -1.3 6,595,674 - +0.2 6,595,899 - AO356_28360 0.10 +1.3 6,595,952 + AO356_28360 0.14 -0.6 6,595,952 + AO356_28360 0.14 +0.1 6,595,952 + AO356_28360 0.14 +0.4 6,595,953 - AO356_28360 0.14 -2.1 6,596,012 + AO356_28360 0.19 -1.2 6,596,012 + AO356_28360 0.19 +0.8 6,596,013 - AO356_28360 0.19 -1.1 6,596,348 + AO356_28360 0.46 -1.3 6,596,348 + AO356_28360 0.46 +0.3 6,596,375 + AO356_28360 0.48 +0.9 6,596,376 - AO356_28360 0.48 +0.5 6,596,408 + AO356_28360 0.50 -1.3 6,596,408 + AO356_28360 0.50 -1.3 6,596,408 + AO356_28360 0.50 +1.0 6,596,409 - AO356_28360 0.51 +0.3 6,596,409 - AO356_28360 0.51 -1.3 6,596,534 + AO356_28360 0.60 -0.9
Or see this region's nucleotide sequence