Experiment: 50% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_28230 and AO356_28235 are separated by 339 nucleotides AO356_28235 and AO356_28240 are separated by 17 nucleotides
AO356_28230: AO356_28230 - syrP protein, at 6,523,290 to 6,524,351
_28230
AO356_28235: AO356_28235 - thioester reductase, at 6,524,691 to 6,526,538
_28235
AO356_28240: AO356_28240 - chemotaxis protein CheX, at 6,526,556 to 6,527,491
_28240
Position (kb)
6524
6525
6526
6527 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 6523.912 kb on + strand, within AO356_28230 at 6524.356 kb on - strand at 6524.593 kb on - strand at 6524.835 kb on + strand at 6524.904 kb on - strand, within AO356_28235 at 6524.904 kb on - strand, within AO356_28235 at 6525.186 kb on + strand, within AO356_28235 at 6525.195 kb on + strand, within AO356_28235 at 6525.195 kb on + strand, within AO356_28235 at 6525.195 kb on + strand, within AO356_28235 at 6525.196 kb on - strand, within AO356_28235 at 6525.196 kb on - strand, within AO356_28235 at 6525.198 kb on - strand, within AO356_28235 at 6525.270 kb on - strand, within AO356_28235 at 6525.627 kb on + strand, within AO356_28235 at 6525.628 kb on - strand, within AO356_28235 at 6525.822 kb on + strand, within AO356_28235 at 6526.087 kb on - strand, within AO356_28235 at 6526.191 kb on + strand, within AO356_28235 at 6526.751 kb on + strand, within AO356_28240 at 6527.088 kb on - strand, within AO356_28240 at 6527.321 kb on + strand, within AO356_28240 at 6527.321 kb on + strand, within AO356_28240 at 6527.322 kb on - strand, within AO356_28240 at 6527.372 kb on + strand, within AO356_28240 at 6527.383 kb on + strand, within AO356_28240 at 6527.399 kb on + strand at 6527.439 kb on - strand at 6527.439 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 50% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine remove 6,523,912 + AO356_28230 0.59 -2.4 6,524,356 - +0.4 6,524,593 - -0.8 6,524,835 + -0.9 6,524,904 - AO356_28235 0.12 +0.4 6,524,904 - AO356_28235 0.12 +0.1 6,525,186 + AO356_28235 0.27 +0.6 6,525,195 + AO356_28235 0.27 -1.5 6,525,195 + AO356_28235 0.27 +0.7 6,525,195 + AO356_28235 0.27 +0.3 6,525,196 - AO356_28235 0.27 -0.1 6,525,196 - AO356_28235 0.27 +0.4 6,525,198 - AO356_28235 0.27 -1.2 6,525,270 - AO356_28235 0.31 +0.5 6,525,627 + AO356_28235 0.51 +0.8 6,525,628 - AO356_28235 0.51 +0.4 6,525,822 + AO356_28235 0.61 -0.1 6,526,087 - AO356_28235 0.76 +1.3 6,526,191 + AO356_28235 0.81 -0.0 6,526,751 + AO356_28240 0.21 +2.0 6,527,088 - AO356_28240 0.57 +0.5 6,527,321 + AO356_28240 0.82 +0.7 6,527,321 + AO356_28240 0.82 +1.3 6,527,322 - AO356_28240 0.82 +0.2 6,527,372 + AO356_28240 0.87 -0.0 6,527,383 + AO356_28240 0.88 -0.3 6,527,399 + +0.5 6,527,439 - +0.3 6,527,439 - +0.6
Or see this region's nucleotide sequence