Experiment: 50% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_22530 and AO356_22535 are separated by 140 nucleotides AO356_22535 and AO356_22540 are separated by 223 nucleotides AO356_22540 and AO356_22545 are separated by 297 nucleotides
AO356_22530: AO356_22530 - LysR family transcriptional regulator, at 5,035,890 to 5,036,807
_22530
AO356_22535: AO356_22535 - ketosteroid isomerase, at 5,036,948 to 5,037,352
_22535
AO356_22540: AO356_22540 - NIPSNAP family containing protein, at 5,037,576 to 5,037,881
_22540
AO356_22545: AO356_22545 - LysR family transcriptional regulator, at 5,038,179 to 5,039,117
_22545
Position (kb) 5036
5037
5038 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5036.116 kb on - strand, within AO356_22530 at 5036.155 kb on + strand, within AO356_22530 at 5036.155 kb on + strand, within AO356_22530 at 5036.179 kb on + strand, within AO356_22530 at 5036.179 kb on + strand, within AO356_22530 at 5036.180 kb on - strand, within AO356_22530 at 5036.180 kb on - strand, within AO356_22530 at 5036.180 kb on - strand, within AO356_22530 at 5036.180 kb on - strand, within AO356_22530 at 5036.251 kb on - strand, within AO356_22530 at 5036.338 kb on + strand, within AO356_22530 at 5036.388 kb on + strand, within AO356_22530 at 5036.388 kb on + strand, within AO356_22530 at 5036.410 kb on - strand, within AO356_22530 at 5036.472 kb on + strand, within AO356_22530 at 5036.472 kb on + strand, within AO356_22530 at 5036.472 kb on + strand, within AO356_22530 at 5036.472 kb on + strand, within AO356_22530 at 5036.472 kb on + strand, within AO356_22530 at 5036.472 kb on + strand, within AO356_22530 at 5036.472 kb on + strand, within AO356_22530 at 5036.473 kb on - strand, within AO356_22530 at 5036.473 kb on - strand, within AO356_22530 at 5036.497 kb on + strand, within AO356_22530 at 5036.498 kb on - strand, within AO356_22530 at 5036.640 kb on + strand, within AO356_22530 at 5036.640 kb on + strand, within AO356_22530 at 5036.640 kb on + strand, within AO356_22530 at 5036.641 kb on - strand, within AO356_22530 at 5036.703 kb on + strand, within AO356_22530 at 5036.726 kb on + strand at 5036.776 kb on + strand at 5036.852 kb on + strand at 5036.859 kb on + strand at 5036.859 kb on + strand at 5036.885 kb on - strand at 5036.924 kb on + strand at 5036.925 kb on - strand at 5036.978 kb on + strand at 5037.110 kb on - strand, within AO356_22535 at 5037.128 kb on - strand, within AO356_22535 at 5037.205 kb on - strand, within AO356_22535 at 5037.205 kb on - strand, within AO356_22535 at 5037.236 kb on + strand, within AO356_22535 at 5037.237 kb on - strand, within AO356_22535 at 5037.248 kb on + strand, within AO356_22535 at 5037.248 kb on + strand, within AO356_22535 at 5037.248 kb on + strand, within AO356_22535 at 5037.248 kb on + strand, within AO356_22535 at 5037.248 kb on + strand, within AO356_22535 at 5037.248 kb on + strand, within AO356_22535 at 5037.248 kb on + strand, within AO356_22535 at 5037.248 kb on + strand, within AO356_22535 at 5037.248 kb on + strand, within AO356_22535 at 5037.248 kb on + strand, within AO356_22535 at 5037.248 kb on + strand, within AO356_22535 at 5037.248 kb on + strand, within AO356_22535 at 5037.249 kb on - strand, within AO356_22535 at 5037.249 kb on - strand, within AO356_22535 at 5037.249 kb on - strand, within AO356_22535 at 5037.249 kb on - strand, within AO356_22535 at 5037.249 kb on - strand, within AO356_22535 at 5037.261 kb on + strand, within AO356_22535 at 5037.356 kb on + strand at 5037.357 kb on - strand at 5037.537 kb on + strand at 5037.538 kb on - strand at 5037.538 kb on - strand at 5037.538 kb on - strand at 5037.538 kb on - strand at 5037.538 kb on - strand at 5037.538 kb on - strand at 5037.558 kb on - strand at 5037.597 kb on + strand at 5037.597 kb on + strand at 5037.597 kb on + strand at 5037.598 kb on - strand at 5037.598 kb on - strand at 5037.598 kb on - strand at 5037.598 kb on - strand at 5037.598 kb on - strand at 5037.601 kb on + strand at 5037.601 kb on + strand at 5037.601 kb on + strand at 5037.601 kb on + strand at 5037.602 kb on - strand at 5037.602 kb on - strand at 5037.602 kb on - strand at 5037.602 kb on - strand at 5037.602 kb on - strand at 5037.636 kb on + strand, within AO356_22540 at 5037.636 kb on + strand, within AO356_22540 at 5037.637 kb on - strand, within AO356_22540 at 5037.668 kb on + strand, within AO356_22540 at 5037.669 kb on - strand, within AO356_22540 at 5037.691 kb on - strand, within AO356_22540 at 5037.691 kb on - strand, within AO356_22540 at 5037.691 kb on - strand, within AO356_22540 at 5037.691 kb on - strand, within AO356_22540 at 5037.726 kb on + strand, within AO356_22540 at 5037.726 kb on + strand, within AO356_22540 at 5037.727 kb on - strand, within AO356_22540 at 5037.760 kb on - strand, within AO356_22540 at 5037.760 kb on - strand, within AO356_22540 at 5037.769 kb on - strand, within AO356_22540 at 5037.788 kb on - strand, within AO356_22540 at 5037.847 kb on - strand, within AO356_22540 at 5037.847 kb on - strand, within AO356_22540 at 5037.847 kb on - strand, within AO356_22540 at 5037.956 kb on - strand at 5037.979 kb on + strand at 5037.979 kb on + strand at 5037.980 kb on - strand at 5037.980 kb on - strand at 5038.015 kb on + strand at 5038.015 kb on + strand at 5038.015 kb on + strand at 5038.016 kb on - strand at 5038.016 kb on - strand at 5038.079 kb on + strand at 5038.079 kb on + strand at 5038.080 kb on - strand at 5038.080 kb on - strand at 5038.116 kb on + strand at 5038.116 kb on + strand at 5038.116 kb on + strand at 5038.116 kb on + strand at 5038.116 kb on + strand at 5038.117 kb on - strand at 5038.123 kb on + strand at 5038.123 kb on + strand at 5038.123 kb on + strand at 5038.124 kb on - strand at 5038.124 kb on - strand at 5038.124 kb on - strand at 5038.124 kb on - strand at 5038.154 kb on + strand at 5038.155 kb on - strand at 5038.155 kb on - strand at 5038.216 kb on + strand at 5038.217 kb on - strand at 5038.217 kb on - strand at 5038.309 kb on + strand, within AO356_22545 at 5038.309 kb on + strand, within AO356_22545 at 5038.309 kb on + strand, within AO356_22545 at 5038.310 kb on - strand, within AO356_22545 at 5038.310 kb on - strand, within AO356_22545 at 5038.310 kb on - strand, within AO356_22545 at 5038.310 kb on - strand, within AO356_22545 at 5038.310 kb on - strand, within AO356_22545 at 5038.310 kb on - strand, within AO356_22545 at 5038.310 kb on - strand, within AO356_22545
Per-strain Table
Position Strand Gene LocusTag Fraction 50% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine remove 5,036,116 - AO356_22530 0.25 +0.2 5,036,155 + AO356_22530 0.29 -0.0 5,036,155 + AO356_22530 0.29 +0.3 5,036,179 + AO356_22530 0.31 +0.6 5,036,179 + AO356_22530 0.31 -0.3 5,036,180 - AO356_22530 0.32 -0.3 5,036,180 - AO356_22530 0.32 +0.3 5,036,180 - AO356_22530 0.32 -0.3 5,036,180 - AO356_22530 0.32 +1.4 5,036,251 - AO356_22530 0.39 +0.1 5,036,338 + AO356_22530 0.49 +0.2 5,036,388 + AO356_22530 0.54 -1.3 5,036,388 + AO356_22530 0.54 +0.4 5,036,410 - AO356_22530 0.57 -0.4 5,036,472 + AO356_22530 0.63 +0.6 5,036,472 + AO356_22530 0.63 -1.1 5,036,472 + AO356_22530 0.63 -0.1 5,036,472 + AO356_22530 0.63 -0.1 5,036,472 + AO356_22530 0.63 -0.1 5,036,472 + AO356_22530 0.63 +1.9 5,036,472 + AO356_22530 0.63 -0.5 5,036,473 - AO356_22530 0.64 -2.5 5,036,473 - AO356_22530 0.64 -2.0 5,036,497 + AO356_22530 0.66 -0.9 5,036,498 - AO356_22530 0.66 -1.0 5,036,640 + AO356_22530 0.82 -2.0 5,036,640 + AO356_22530 0.82 -0.1 5,036,640 + AO356_22530 0.82 -0.1 5,036,641 - AO356_22530 0.82 -0.5 5,036,703 + AO356_22530 0.89 -0.0 5,036,726 + +0.7 5,036,776 + +0.7 5,036,852 + +0.2 5,036,859 + +0.9 5,036,859 + -1.4 5,036,885 - -1.1 5,036,924 + +0.3 5,036,925 - +0.6 5,036,978 + +0.1 5,037,110 - AO356_22535 0.40 -0.4 5,037,128 - AO356_22535 0.44 +0.9 5,037,205 - AO356_22535 0.63 -1.0 5,037,205 - AO356_22535 0.63 -0.4 5,037,236 + AO356_22535 0.71 -1.1 5,037,237 - AO356_22535 0.71 -1.3 5,037,248 + AO356_22535 0.74 +0.4 5,037,248 + AO356_22535 0.74 +0.1 5,037,248 + AO356_22535 0.74 +0.2 5,037,248 + AO356_22535 0.74 +1.0 5,037,248 + AO356_22535 0.74 +0.2 5,037,248 + AO356_22535 0.74 +0.9 5,037,248 + AO356_22535 0.74 -0.6 5,037,248 + AO356_22535 0.74 -1.0 5,037,248 + AO356_22535 0.74 -0.9 5,037,248 + AO356_22535 0.74 -0.0 5,037,248 + AO356_22535 0.74 -0.1 5,037,248 + AO356_22535 0.74 -1.3 5,037,249 - AO356_22535 0.74 -0.0 5,037,249 - AO356_22535 0.74 +0.4 5,037,249 - AO356_22535 0.74 -1.7 5,037,249 - AO356_22535 0.74 -0.7 5,037,249 - AO356_22535 0.74 +0.5 5,037,261 + AO356_22535 0.77 -0.5 5,037,356 + -0.6 5,037,357 - +0.2 5,037,537 + +0.9 5,037,538 - -0.6 5,037,538 - -0.1 5,037,538 - -0.1 5,037,538 - -0.6 5,037,538 - +1.2 5,037,538 - +0.9 5,037,558 - +0.4 5,037,597 + -1.0 5,037,597 + +0.9 5,037,597 + +1.0 5,037,598 - -0.8 5,037,598 - -1.1 5,037,598 - -0.5 5,037,598 - +1.2 5,037,598 - +0.2 5,037,601 + +0.7 5,037,601 + -0.6 5,037,601 + -0.1 5,037,601 + +0.4 5,037,602 - -0.1 5,037,602 - +2.2 5,037,602 - +0.3 5,037,602 - +0.2 5,037,602 - +0.7 5,037,636 + AO356_22540 0.20 -0.9 5,037,636 + AO356_22540 0.20 +0.9 5,037,637 - AO356_22540 0.20 -0.6 5,037,668 + AO356_22540 0.30 -0.3 5,037,669 - AO356_22540 0.30 +0.4 5,037,691 - AO356_22540 0.38 +0.2 5,037,691 - AO356_22540 0.38 +0.4 5,037,691 - AO356_22540 0.38 -1.0 5,037,691 - AO356_22540 0.38 -0.4 5,037,726 + AO356_22540 0.49 -0.1 5,037,726 + AO356_22540 0.49 -0.4 5,037,727 - AO356_22540 0.49 +0.3 5,037,760 - AO356_22540 0.60 -0.5 5,037,760 - AO356_22540 0.60 -1.1 5,037,769 - AO356_22540 0.63 -0.3 5,037,788 - AO356_22540 0.69 +0.0 5,037,847 - AO356_22540 0.89 -0.3 5,037,847 - AO356_22540 0.89 +0.4 5,037,847 - AO356_22540 0.89 +0.3 5,037,956 - +0.2 5,037,979 + -1.0 5,037,979 + -0.1 5,037,980 - +0.2 5,037,980 - -0.4 5,038,015 + +0.3 5,038,015 + -0.1 5,038,015 + +0.4 5,038,016 - +0.4 5,038,016 - +0.6 5,038,079 + +0.9 5,038,079 + +1.4 5,038,080 - -0.7 5,038,080 - -2.0 5,038,116 + +1.8 5,038,116 + +1.4 5,038,116 + +0.7 5,038,116 + -0.4 5,038,116 + -0.5 5,038,117 - -0.4 5,038,123 + +0.2 5,038,123 + -0.8 5,038,123 + +0.1 5,038,124 - -1.0 5,038,124 - -0.5 5,038,124 - -1.0 5,038,124 - +0.3 5,038,154 + -0.3 5,038,155 - +0.4 5,038,155 - +1.0 5,038,216 + -0.1 5,038,217 - -0.4 5,038,217 - -0.5 5,038,309 + AO356_22545 0.14 +1.0 5,038,309 + AO356_22545 0.14 +0.3 5,038,309 + AO356_22545 0.14 -1.3 5,038,310 - AO356_22545 0.14 +0.4 5,038,310 - AO356_22545 0.14 +0.4 5,038,310 - AO356_22545 0.14 -1.3 5,038,310 - AO356_22545 0.14 +0.4 5,038,310 - AO356_22545 0.14 -0.0 5,038,310 - AO356_22545 0.14 +0.3 5,038,310 - AO356_22545 0.14 +0.2
Or see this region's nucleotide sequence