Experiment: 50% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_21840 and AO356_21845 are separated by 224 nucleotides AO356_21845 and AO356_21850 overlap by 1 nucleotides
AO356_21840: AO356_21840 - N-methylproline demethylase, at 4,889,279 to 4,891,315
_21840
AO356_21845: AO356_21845 - electron transporter RnfB, at 4,891,540 to 4,892,289
_21845
AO356_21850: AO356_21850 - electron transfer flavoprotein subunit beta, at 4,892,289 to 4,893,224
_21850
Position (kb)
4891
4892
4893 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4890.750 kb on + strand, within AO356_21840 at 4890.867 kb on + strand, within AO356_21840 at 4890.868 kb on - strand, within AO356_21840 at 4891.087 kb on - strand, within AO356_21840 at 4891.087 kb on - strand, within AO356_21840 at 4891.188 kb on + strand at 4891.189 kb on - strand at 4891.285 kb on + strand at 4891.285 kb on + strand at 4891.286 kb on - strand at 4891.287 kb on + strand at 4891.287 kb on + strand at 4891.287 kb on + strand at 4891.287 kb on + strand at 4891.440 kb on + strand at 4891.441 kb on - strand at 4891.441 kb on - strand at 4891.527 kb on + strand at 4891.838 kb on - strand, within AO356_21845 at 4891.963 kb on + strand, within AO356_21845 at 4891.963 kb on + strand, within AO356_21845 at 4891.963 kb on + strand, within AO356_21845 at 4891.963 kb on + strand, within AO356_21845 at 4891.963 kb on + strand, within AO356_21845 at 4891.963 kb on + strand, within AO356_21845 at 4891.963 kb on + strand, within AO356_21845 at 4891.964 kb on - strand, within AO356_21845 at 4891.964 kb on - strand, within AO356_21845 at 4891.964 kb on - strand, within AO356_21845 at 4891.964 kb on - strand, within AO356_21845 at 4891.964 kb on - strand, within AO356_21845 at 4892.093 kb on - strand, within AO356_21845 at 4892.189 kb on + strand, within AO356_21845 at 4892.190 kb on - strand, within AO356_21845 at 4892.192 kb on + strand, within AO356_21845 at 4892.260 kb on - strand at 4892.339 kb on + strand at 4892.339 kb on + strand at 4892.340 kb on - strand at 4892.340 kb on - strand at 4892.546 kb on + strand, within AO356_21850 at 4892.546 kb on + strand, within AO356_21850 at 4892.546 kb on + strand, within AO356_21850 at 4892.546 kb on + strand, within AO356_21850 at 4892.547 kb on - strand, within AO356_21850 at 4892.547 kb on - strand, within AO356_21850 at 4892.547 kb on - strand, within AO356_21850 at 4892.588 kb on - strand, within AO356_21850 at 4892.588 kb on - strand, within AO356_21850 at 4892.588 kb on - strand, within AO356_21850 at 4892.676 kb on + strand, within AO356_21850 at 4892.676 kb on + strand, within AO356_21850 at 4892.677 kb on - strand, within AO356_21850 at 4892.792 kb on - strand, within AO356_21850 at 4893.054 kb on + strand, within AO356_21850 at 4893.054 kb on + strand, within AO356_21850 at 4893.055 kb on - strand, within AO356_21850 at 4893.055 kb on - strand, within AO356_21850 at 4893.168 kb on + strand at 4893.168 kb on + strand at 4893.168 kb on + strand at 4893.168 kb on + strand at 4893.168 kb on + strand at 4893.168 kb on + strand at 4893.168 kb on + strand at 4893.168 kb on + strand at 4893.169 kb on - strand at 4893.169 kb on - strand at 4893.169 kb on - strand at 4893.169 kb on - strand at 4893.169 kb on - strand at 4893.226 kb on + strand at 4893.227 kb on - strand at 4893.227 kb on - strand at 4893.230 kb on + strand at 4893.231 kb on - strand at 4893.231 kb on - strand at 4893.231 kb on - strand at 4893.231 kb on - strand at 4893.238 kb on + strand at 4893.239 kb on - strand at 4893.280 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 50% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine remove 4,890,750 + AO356_21840 0.72 -0.8 4,890,867 + AO356_21840 0.78 -0.3 4,890,868 - AO356_21840 0.78 +0.5 4,891,087 - AO356_21840 0.89 -1.2 4,891,087 - AO356_21840 0.89 -0.9 4,891,188 + -0.1 4,891,189 - +0.0 4,891,285 + -1.3 4,891,285 + +0.1 4,891,286 - +1.0 4,891,287 + -0.1 4,891,287 + +0.5 4,891,287 + -0.7 4,891,287 + -1.0 4,891,440 + -1.4 4,891,441 - +0.2 4,891,441 - -0.4 4,891,527 + -0.1 4,891,838 - AO356_21845 0.40 +0.1 4,891,963 + AO356_21845 0.56 +0.8 4,891,963 + AO356_21845 0.56 +0.2 4,891,963 + AO356_21845 0.56 -1.0 4,891,963 + AO356_21845 0.56 -1.4 4,891,963 + AO356_21845 0.56 -0.4 4,891,963 + AO356_21845 0.56 +1.7 4,891,963 + AO356_21845 0.56 -0.1 4,891,964 - AO356_21845 0.57 +0.4 4,891,964 - AO356_21845 0.57 -0.9 4,891,964 - AO356_21845 0.57 +0.7 4,891,964 - AO356_21845 0.57 +2.2 4,891,964 - AO356_21845 0.57 -2.1 4,892,093 - AO356_21845 0.74 -1.5 4,892,189 + AO356_21845 0.87 +0.2 4,892,190 - AO356_21845 0.87 -0.5 4,892,192 + AO356_21845 0.87 -0.0 4,892,260 - +0.1 4,892,339 + -0.8 4,892,339 + -0.6 4,892,340 - -0.1 4,892,340 - +1.2 4,892,546 + AO356_21850 0.27 +1.3 4,892,546 + AO356_21850 0.27 +1.9 4,892,546 + AO356_21850 0.27 +1.9 4,892,546 + AO356_21850 0.27 -0.5 4,892,547 - AO356_21850 0.28 -3.5 4,892,547 - AO356_21850 0.28 -0.4 4,892,547 - AO356_21850 0.28 +0.3 4,892,588 - AO356_21850 0.32 +2.9 4,892,588 - AO356_21850 0.32 -0.3 4,892,588 - AO356_21850 0.32 -0.4 4,892,676 + AO356_21850 0.41 +0.9 4,892,676 + AO356_21850 0.41 -1.6 4,892,677 - AO356_21850 0.41 +0.6 4,892,792 - AO356_21850 0.54 +0.5 4,893,054 + AO356_21850 0.82 -1.0 4,893,054 + AO356_21850 0.82 +2.2 4,893,055 - AO356_21850 0.82 -0.1 4,893,055 - AO356_21850 0.82 +0.1 4,893,168 + +0.3 4,893,168 + +0.9 4,893,168 + -0.1 4,893,168 + +0.4 4,893,168 + -0.5 4,893,168 + -0.3 4,893,168 + -0.6 4,893,168 + -0.3 4,893,169 - +1.0 4,893,169 - -0.4 4,893,169 - -1.1 4,893,169 - +1.2 4,893,169 - +0.5 4,893,226 + -2.1 4,893,227 - +0.5 4,893,227 - -1.5 4,893,230 + -0.3 4,893,231 - -0.9 4,893,231 - -0.4 4,893,231 - -0.8 4,893,231 - -0.6 4,893,238 + -0.4 4,893,239 - +0.5 4,893,280 - -0.3
Or see this region's nucleotide sequence