Experiment: 50% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_19510 and AO356_19515 are separated by 73 nucleotides AO356_19515 and AO356_19520 are separated by 130 nucleotides AO356_19520 and AO356_19525 are separated by 254 nucleotides
AO356_19510: AO356_19510 - DNA mismatch repair protein MutS, at 4,377,986 to 4,378,543
_19510
AO356_19515: AO356_19515 - hypothetical protein, at 4,378,617 to 4,378,937
_19515
AO356_19520: AO356_19520 - isochorismatase, at 4,379,068 to 4,379,658
_19520
AO356_19525: AO356_19525 - protein-(glutamine-N5) methyltransferase, ribosomal protein L3-specific, at 4,379,913 to 4,380,821
_19525
Position (kb)
4379
4380 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 4378.192 kb on + strand, within AO356_19510 at 4378.193 kb on - strand, within AO356_19510 at 4378.497 kb on - strand at 4378.497 kb on - strand at 4378.717 kb on - strand, within AO356_19515 at 4378.717 kb on - strand, within AO356_19515 at 4378.979 kb on + strand at 4378.979 kb on + strand at 4379.168 kb on + strand, within AO356_19520 at 4379.168 kb on + strand, within AO356_19520 at 4379.168 kb on + strand, within AO356_19520 at 4379.168 kb on + strand, within AO356_19520 at 4379.169 kb on - strand, within AO356_19520 at 4379.169 kb on - strand, within AO356_19520 at 4379.169 kb on - strand, within AO356_19520 at 4379.559 kb on - strand, within AO356_19520 at 4379.616 kb on - strand at 4379.753 kb on + strand at 4379.753 kb on + strand at 4379.785 kb on + strand at 4379.785 kb on + strand at 4379.785 kb on + strand at 4379.785 kb on + strand at 4379.785 kb on + strand at 4379.786 kb on - strand at 4379.786 kb on - strand at 4379.786 kb on - strand at 4379.786 kb on - strand at 4379.786 kb on - strand at 4379.786 kb on - strand at 4379.786 kb on - strand at 4379.786 kb on - strand at 4379.806 kb on - strand at 4379.878 kb on - strand at 4379.948 kb on + strand at 4379.948 kb on + strand at 4380.074 kb on + strand, within AO356_19525 at 4380.074 kb on + strand, within AO356_19525 at 4380.074 kb on + strand, within AO356_19525 at 4380.078 kb on + strand, within AO356_19525 at 4380.078 kb on + strand, within AO356_19525 at 4380.078 kb on + strand, within AO356_19525 at 4380.079 kb on - strand, within AO356_19525 at 4380.165 kb on + strand, within AO356_19525 at 4380.165 kb on + strand, within AO356_19525 at 4380.165 kb on + strand, within AO356_19525
Per-strain Table
Position Strand Gene LocusTag Fraction 50% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine remove 4,378,192 + AO356_19510 0.37 -0.4 4,378,193 - AO356_19510 0.37 +1.2 4,378,497 - +0.6 4,378,497 - +0.3 4,378,717 - AO356_19515 0.31 -0.0 4,378,717 - AO356_19515 0.31 -0.2 4,378,979 + -2.0 4,378,979 + -1.3 4,379,168 + AO356_19520 0.17 -2.0 4,379,168 + AO356_19520 0.17 -1.6 4,379,168 + AO356_19520 0.17 -3.6 4,379,168 + AO356_19520 0.17 -2.0 4,379,169 - AO356_19520 0.17 -3.2 4,379,169 - AO356_19520 0.17 -2.4 4,379,169 - AO356_19520 0.17 -4.0 4,379,559 - AO356_19520 0.83 -2.0 4,379,616 - -2.4 4,379,753 + -1.0 4,379,753 + -1.3 4,379,785 + -0.5 4,379,785 + -0.8 4,379,785 + -0.3 4,379,785 + -1.5 4,379,785 + -2.4 4,379,786 - -0.5 4,379,786 - -0.4 4,379,786 - -0.2 4,379,786 - -2.6 4,379,786 - -0.4 4,379,786 - -0.0 4,379,786 - -0.0 4,379,786 - +0.6 4,379,806 - -0.7 4,379,878 - +1.0 4,379,948 + +0.6 4,379,948 + -2.0 4,380,074 + AO356_19525 0.18 +0.3 4,380,074 + AO356_19525 0.18 +0.8 4,380,074 + AO356_19525 0.18 +0.2 4,380,078 + AO356_19525 0.18 +1.0 4,380,078 + AO356_19525 0.18 -0.0 4,380,078 + AO356_19525 0.18 +0.0 4,380,079 - AO356_19525 0.18 -0.9 4,380,165 + AO356_19525 0.28 +1.4 4,380,165 + AO356_19525 0.28 +0.8 4,380,165 + AO356_19525 0.28 +0.2
Or see this region's nucleotide sequence