Experiment: 50% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_15355 and AO356_15360 are separated by 72 nucleotides AO356_15360 and AO356_15365 are separated by 227 nucleotides AO356_15365 and AO356_15370 are separated by 39 nucleotides AO356_15370 and AO356_15375 are separated by 90 nucleotides
AO356_15355: AO356_15355 - murein transglycosylase, at 3,477,427 to 3,478,749
_15355
AO356_15360: AO356_15360 - lipoyl synthase, at 3,478,822 to 3,478,986
_15360
AO356_15365: AO356_15365 - DNA polymerase III subunit delta, at 3,479,214 to 3,480,251
_15365
AO356_15370: AO356_15370 - hypothetical protein, at 3,480,291 to 3,480,896
_15370
AO356_15375: AO356_15375 - leucine--tRNA ligase, at 3,480,987 to 3,483,593
_15375
Position (kb)
3479
3480
3481 Strain fitness (log2 ratio)
-1
0
1
2 at 3478.301 kb on + strand, within AO356_15355 at 3478.394 kb on + strand, within AO356_15355 at 3478.394 kb on + strand, within AO356_15355 at 3478.394 kb on + strand, within AO356_15355 at 3478.395 kb on - strand, within AO356_15355 at 3478.660 kb on - strand at 3478.765 kb on + strand at 3478.765 kb on + strand at 3478.766 kb on - strand at 3478.785 kb on + strand at 3478.785 kb on + strand at 3478.785 kb on + strand at 3478.786 kb on - strand at 3478.786 kb on - strand at 3478.786 kb on - strand at 3478.804 kb on - strand at 3478.880 kb on + strand, within AO356_15360 at 3479.215 kb on + strand at 3480.252 kb on - strand at 3480.252 kb on - strand at 3480.252 kb on - strand at 3480.293 kb on - strand at 3480.293 kb on - strand at 3480.919 kb on - strand at 3480.951 kb on - strand at 3480.951 kb on - strand at 3480.951 kb on - strand at 3480.951 kb on - strand at 3480.989 kb on - strand at 3480.989 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 50% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine remove 3,478,301 + AO356_15355 0.66 -0.5 3,478,394 + AO356_15355 0.73 +2.2 3,478,394 + AO356_15355 0.73 -0.8 3,478,394 + AO356_15355 0.73 +0.2 3,478,395 - AO356_15355 0.73 +0.6 3,478,660 - -0.2 3,478,765 + -1.0 3,478,765 + +0.2 3,478,766 - -0.5 3,478,785 + +0.2 3,478,785 + -0.2 3,478,785 + +0.2 3,478,786 - -0.2 3,478,786 - +0.8 3,478,786 - +1.4 3,478,804 - +1.2 3,478,880 + AO356_15360 0.35 -1.4 3,479,215 + +0.8 3,480,252 - -0.1 3,480,252 - -0.8 3,480,252 - -0.8 3,480,293 - +0.2 3,480,293 - +0.2 3,480,919 - +0.6 3,480,951 - +1.1 3,480,951 - +0.2 3,480,951 - +0.6 3,480,951 - -0.8 3,480,989 - +0.2 3,480,989 - +0.3
Or see this region's nucleotide sequence