Experiment: BG11 with Chloride 62.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fni and dacB are separated by 5 nucleotides dacB and pilD are separated by 241 nucleotides
Synpcc7942_1933: fni - isopentenyl pyrophosphate isomerase, at 2,006,589 to 2,007,635
fni
Synpcc7942_1934: dacB - D-alanyl-D-alanine carboxypeptidase/D-alanyl-D-alanine-endopeptidase, at 2,007,641 to 2,008,990
dacB
Synpcc7942_1935: pilD - type 4 prepilin peptidase 1. Aspartic peptidase. MEROPS family A24A, at 2,009,232 to 2,010,020
pilD
Position (kb)
2007
2008
2009 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5
6 at 2006.682 kb on - strand at 2006.682 kb on - strand at 2006.716 kb on + strand, within fni at 2006.716 kb on + strand, within fni at 2006.722 kb on - strand, within fni at 2006.758 kb on - strand, within fni at 2006.765 kb on + strand, within fni at 2006.773 kb on - strand, within fni at 2006.790 kb on + strand, within fni at 2006.853 kb on - strand, within fni at 2006.885 kb on + strand at 2006.902 kb on + strand, within fni at 2006.910 kb on - strand, within fni at 2006.910 kb on - strand, within fni at 2006.936 kb on - strand, within fni at 2006.989 kb on - strand, within fni at 2006.999 kb on - strand, within fni at 2006.999 kb on - strand, within fni at 2007.017 kb on + strand, within fni at 2007.021 kb on - strand, within fni at 2007.081 kb on + strand, within fni at 2007.081 kb on + strand, within fni at 2007.083 kb on + strand, within fni at 2007.104 kb on + strand, within fni at 2007.112 kb on - strand, within fni at 2007.146 kb on + strand, within fni at 2007.154 kb on - strand, within fni at 2007.198 kb on - strand, within fni at 2007.205 kb on - strand, within fni at 2007.212 kb on - strand, within fni at 2007.242 kb on - strand, within fni at 2007.248 kb on + strand, within fni at 2007.279 kb on - strand, within fni at 2007.279 kb on - strand, within fni at 2007.476 kb on - strand, within fni at 2007.476 kb on - strand, within fni at 2007.498 kb on - strand, within fni at 2007.499 kb on - strand, within fni at 2007.522 kb on + strand, within fni at 2007.565 kb on - strand at 2007.567 kb on + strand at 2007.647 kb on + strand at 2007.675 kb on - strand at 2007.675 kb on - strand at 2007.690 kb on - strand at 2007.697 kb on - strand at 2007.705 kb on + strand at 2007.713 kb on + strand at 2007.713 kb on - strand at 2007.789 kb on - strand, within dacB at 2007.840 kb on + strand, within dacB at 2007.840 kb on + strand, within dacB at 2007.840 kb on + strand, within dacB at 2007.848 kb on - strand, within dacB at 2007.848 kb on - strand at 2007.861 kb on + strand, within dacB at 2007.893 kb on - strand, within dacB at 2007.949 kb on + strand, within dacB at 2007.997 kb on - strand, within dacB at 2007.998 kb on - strand, within dacB at 2008.012 kb on + strand, within dacB at 2008.020 kb on - strand, within dacB at 2008.042 kb on - strand, within dacB at 2008.051 kb on + strand, within dacB at 2008.051 kb on + strand, within dacB at 2008.051 kb on + strand, within dacB at 2008.051 kb on + strand, within dacB at 2008.059 kb on - strand, within dacB at 2008.062 kb on - strand, within dacB at 2008.074 kb on - strand, within dacB at 2008.094 kb on + strand, within dacB at 2008.141 kb on - strand, within dacB at 2008.154 kb on + strand, within dacB at 2008.181 kb on + strand, within dacB at 2008.210 kb on - strand, within dacB at 2008.259 kb on - strand, within dacB at 2008.259 kb on - strand, within dacB at 2008.265 kb on + strand, within dacB at 2008.336 kb on - strand, within dacB at 2008.377 kb on + strand, within dacB at 2008.386 kb on + strand, within dacB at 2008.406 kb on + strand, within dacB at 2008.430 kb on + strand, within dacB at 2008.430 kb on + strand, within dacB at 2008.430 kb on + strand, within dacB at 2008.430 kb on + strand, within dacB at 2008.438 kb on - strand, within dacB at 2008.438 kb on - strand, within dacB at 2008.438 kb on - strand, within dacB at 2008.438 kb on - strand, within dacB at 2008.463 kb on + strand, within dacB at 2008.471 kb on - strand, within dacB at 2008.471 kb on - strand, within dacB at 2008.491 kb on + strand, within dacB at 2008.491 kb on + strand at 2008.491 kb on + strand, within dacB at 2008.498 kb on + strand, within dacB at 2008.498 kb on + strand, within dacB at 2008.498 kb on + strand, within dacB at 2008.498 kb on - strand, within dacB at 2008.498 kb on - strand, within dacB at 2008.499 kb on - strand, within dacB at 2008.514 kb on + strand at 2008.532 kb on - strand, within dacB at 2008.551 kb on - strand, within dacB at 2008.551 kb on - strand, within dacB at 2008.583 kb on + strand, within dacB at 2008.583 kb on + strand, within dacB at 2008.583 kb on + strand, within dacB at 2008.583 kb on + strand, within dacB at 2008.583 kb on + strand at 2008.591 kb on - strand, within dacB at 2008.591 kb on - strand, within dacB at 2008.591 kb on - strand, within dacB at 2008.591 kb on - strand, within dacB at 2008.591 kb on - strand, within dacB at 2008.591 kb on - strand, within dacB at 2008.591 kb on - strand, within dacB at 2008.601 kb on - strand, within dacB at 2008.615 kb on + strand, within dacB at 2008.615 kb on + strand, within dacB at 2008.623 kb on - strand, within dacB at 2008.623 kb on - strand, within dacB at 2008.649 kb on + strand, within dacB at 2008.652 kb on - strand, within dacB at 2008.652 kb on - strand, within dacB at 2008.657 kb on - strand, within dacB at 2008.657 kb on - strand, within dacB at 2008.707 kb on - strand, within dacB at 2008.717 kb on - strand, within dacB at 2008.733 kb on - strand, within dacB at 2008.733 kb on - strand, within dacB at 2008.735 kb on + strand, within dacB at 2008.740 kb on + strand, within dacB at 2008.748 kb on - strand, within dacB at 2008.801 kb on - strand, within dacB at 2008.811 kb on + strand, within dacB at 2008.842 kb on + strand, within dacB at 2008.852 kb on + strand, within dacB at 2009.004 kb on - strand at 2009.266 kb on - strand at 2009.283 kb on - strand at 2009.283 kb on - strand at 2009.304 kb on + strand at 2009.304 kb on + strand at 2009.312 kb on - strand, within pilD at 2009.338 kb on + strand, within pilD at 2009.346 kb on - strand, within pilD at 2009.346 kb on - strand, within pilD at 2009.346 kb on - strand, within pilD at 2009.346 kb on - strand, within pilD at 2009.347 kb on - strand, within pilD at 2009.347 kb on - strand, within pilD at 2009.360 kb on + strand, within pilD at 2009.368 kb on - strand, within pilD at 2009.385 kb on + strand, within pilD at 2009.395 kb on + strand, within pilD at 2009.395 kb on + strand, within pilD at 2009.403 kb on - strand, within pilD at 2009.403 kb on - strand, within pilD at 2009.403 kb on - strand, within pilD at 2009.403 kb on - strand, within pilD at 2009.432 kb on + strand, within pilD at 2009.432 kb on + strand, within pilD at 2009.440 kb on - strand, within pilD at 2009.516 kb on + strand, within pilD at 2009.519 kb on - strand, within pilD at 2009.522 kb on - strand, within pilD at 2009.557 kb on + strand, within pilD at 2009.565 kb on - strand, within pilD at 2009.565 kb on - strand, within pilD at 2009.567 kb on + strand, within pilD at 2009.570 kb on - strand, within pilD at 2009.575 kb on - strand, within pilD at 2009.608 kb on + strand, within pilD at 2009.608 kb on + strand, within pilD at 2009.614 kb on + strand, within pilD at 2009.616 kb on - strand at 2009.625 kb on + strand, within pilD at 2009.646 kb on + strand, within pilD at 2009.649 kb on - strand, within pilD at 2009.652 kb on + strand, within pilD at 2009.659 kb on + strand, within pilD at 2009.667 kb on + strand, within pilD at 2009.669 kb on + strand, within pilD at 2009.669 kb on + strand, within pilD at 2009.669 kb on + strand, within pilD at 2009.669 kb on - strand, within pilD at 2009.731 kb on - strand, within pilD at 2009.762 kb on + strand, within pilD at 2009.762 kb on + strand, within pilD at 2009.777 kb on - strand, within pilD at 2009.810 kb on - strand, within pilD at 2009.827 kb on + strand, within pilD at 2009.845 kb on - strand, within pilD at 2009.845 kb on - strand, within pilD at 2009.891 kb on + strand, within pilD at 2009.904 kb on - strand, within pilD at 2009.925 kb on - strand, within pilD at 2009.964 kb on + strand at 2009.979 kb on + strand at 2009.987 kb on - strand at 2009.987 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BG11 with Chloride 62.5 mM remove 2,006,682 - -1.9 2,006,682 - -1.7 2,006,716 + fni Synpcc7942_1933 0.12 -1.5 2,006,716 + fni Synpcc7942_1933 0.12 +0.9 2,006,722 - fni Synpcc7942_1933 0.13 -2.3 2,006,758 - fni Synpcc7942_1933 0.16 -0.7 2,006,765 + fni Synpcc7942_1933 0.17 -2.3 2,006,773 - fni Synpcc7942_1933 0.18 -4.1 2,006,790 + fni Synpcc7942_1933 0.19 -1.1 2,006,853 - fni Synpcc7942_1933 0.25 -2.9 2,006,885 + +0.9 2,006,902 + fni Synpcc7942_1933 0.30 -3.2 2,006,910 - fni Synpcc7942_1933 0.31 -2.1 2,006,910 - fni Synpcc7942_1933 0.31 -1.4 2,006,936 - fni Synpcc7942_1933 0.33 -2.3 2,006,989 - fni Synpcc7942_1933 0.38 -0.6 2,006,999 - fni Synpcc7942_1933 0.39 -1.2 2,006,999 - fni Synpcc7942_1933 0.39 -1.8 2,007,017 + fni Synpcc7942_1933 0.41 -1.4 2,007,021 - fni Synpcc7942_1933 0.41 -1.5 2,007,081 + fni Synpcc7942_1933 0.47 -0.9 2,007,081 + fni Synpcc7942_1933 0.47 +0.2 2,007,083 + fni Synpcc7942_1933 0.47 -1.6 2,007,104 + fni Synpcc7942_1933 0.49 -1.2 2,007,112 - fni Synpcc7942_1933 0.50 -3.5 2,007,146 + fni Synpcc7942_1933 0.53 -3.4 2,007,154 - fni Synpcc7942_1933 0.54 -2.1 2,007,198 - fni Synpcc7942_1933 0.58 -2.2 2,007,205 - fni Synpcc7942_1933 0.59 +0.1 2,007,212 - fni Synpcc7942_1933 0.60 +1.5 2,007,242 - fni Synpcc7942_1933 0.62 -1.8 2,007,248 + fni Synpcc7942_1933 0.63 -1.5 2,007,279 - fni Synpcc7942_1933 0.66 -1.7 2,007,279 - fni Synpcc7942_1933 0.66 -2.4 2,007,476 - fni Synpcc7942_1933 0.85 -2.0 2,007,476 - fni Synpcc7942_1933 0.85 -2.2 2,007,498 - fni Synpcc7942_1933 0.87 -1.9 2,007,499 - fni Synpcc7942_1933 0.87 -2.7 2,007,522 + fni Synpcc7942_1933 0.89 -3.0 2,007,565 - -2.2 2,007,567 + -0.4 2,007,647 + +0.5 2,007,675 - -1.4 2,007,675 - +0.6 2,007,690 - -0.6 2,007,697 - -0.1 2,007,705 + -0.9 2,007,713 + -1.2 2,007,713 - -0.4 2,007,789 - dacB Synpcc7942_1934 0.11 -2.9 2,007,840 + dacB Synpcc7942_1934 0.15 -0.8 2,007,840 + dacB Synpcc7942_1934 0.15 +0.4 2,007,840 + dacB Synpcc7942_1934 0.15 +1.9 2,007,848 - dacB Synpcc7942_1934 0.15 -2.0 2,007,848 - +0.2 2,007,861 + dacB Synpcc7942_1934 0.16 -0.4 2,007,893 - dacB Synpcc7942_1934 0.19 -1.5 2,007,949 + dacB Synpcc7942_1934 0.23 -2.2 2,007,997 - dacB Synpcc7942_1934 0.26 -0.6 2,007,998 - dacB Synpcc7942_1934 0.26 -1.0 2,008,012 + dacB Synpcc7942_1934 0.27 -0.2 2,008,020 - dacB Synpcc7942_1934 0.28 -1.1 2,008,042 - dacB Synpcc7942_1934 0.30 +0.2 2,008,051 + dacB Synpcc7942_1934 0.30 -0.5 2,008,051 + dacB Synpcc7942_1934 0.30 +0.2 2,008,051 + dacB Synpcc7942_1934 0.30 -0.5 2,008,051 + dacB Synpcc7942_1934 0.30 -0.7 2,008,059 - dacB Synpcc7942_1934 0.31 -1.0 2,008,062 - dacB Synpcc7942_1934 0.31 +0.0 2,008,074 - dacB Synpcc7942_1934 0.32 -2.3 2,008,094 + dacB Synpcc7942_1934 0.34 +0.4 2,008,141 - dacB Synpcc7942_1934 0.37 +0.7 2,008,154 + dacB Synpcc7942_1934 0.38 -1.9 2,008,181 + dacB Synpcc7942_1934 0.40 -1.5 2,008,210 - dacB Synpcc7942_1934 0.42 -1.1 2,008,259 - dacB Synpcc7942_1934 0.46 +1.0 2,008,259 - dacB Synpcc7942_1934 0.46 +0.1 2,008,265 + dacB Synpcc7942_1934 0.46 +0.7 2,008,336 - dacB Synpcc7942_1934 0.51 -0.6 2,008,377 + dacB Synpcc7942_1934 0.55 -1.4 2,008,386 + dacB Synpcc7942_1934 0.55 +0.7 2,008,406 + dacB Synpcc7942_1934 0.57 -2.9 2,008,430 + dacB Synpcc7942_1934 0.58 -0.2 2,008,430 + dacB Synpcc7942_1934 0.58 +0.9 2,008,430 + dacB Synpcc7942_1934 0.58 -4.2 2,008,430 + dacB Synpcc7942_1934 0.58 +0.7 2,008,438 - dacB Synpcc7942_1934 0.59 +0.1 2,008,438 - dacB Synpcc7942_1934 0.59 +0.3 2,008,438 - dacB Synpcc7942_1934 0.59 -0.0 2,008,438 - dacB Synpcc7942_1934 0.59 +0.9 2,008,463 + dacB Synpcc7942_1934 0.61 -1.7 2,008,471 - dacB Synpcc7942_1934 0.61 +0.2 2,008,471 - dacB Synpcc7942_1934 0.61 -0.1 2,008,491 + dacB Synpcc7942_1934 0.63 -0.2 2,008,491 + +2.5 2,008,491 + dacB Synpcc7942_1934 0.63 -1.1 2,008,498 + dacB Synpcc7942_1934 0.63 +1.4 2,008,498 + dacB Synpcc7942_1934 0.63 -0.9 2,008,498 + dacB Synpcc7942_1934 0.63 +0.3 2,008,498 - dacB Synpcc7942_1934 0.63 +1.3 2,008,498 - dacB Synpcc7942_1934 0.63 +0.2 2,008,499 - dacB Synpcc7942_1934 0.64 -0.0 2,008,514 + +0.7 2,008,532 - dacB Synpcc7942_1934 0.66 +1.5 2,008,551 - dacB Synpcc7942_1934 0.67 -1.8 2,008,551 - dacB Synpcc7942_1934 0.67 +1.3 2,008,583 + dacB Synpcc7942_1934 0.70 -0.1 2,008,583 + dacB Synpcc7942_1934 0.70 +1.2 2,008,583 + dacB Synpcc7942_1934 0.70 -0.3 2,008,583 + dacB Synpcc7942_1934 0.70 +0.2 2,008,583 + +1.9 2,008,591 - dacB Synpcc7942_1934 0.70 +1.7 2,008,591 - dacB Synpcc7942_1934 0.70 +6.0 2,008,591 - dacB Synpcc7942_1934 0.70 +0.6 2,008,591 - dacB Synpcc7942_1934 0.70 -2.9 2,008,591 - dacB Synpcc7942_1934 0.70 -0.4 2,008,591 - dacB Synpcc7942_1934 0.70 +0.6 2,008,591 - dacB Synpcc7942_1934 0.70 +1.8 2,008,601 - dacB Synpcc7942_1934 0.71 -0.2 2,008,615 + dacB Synpcc7942_1934 0.72 -1.2 2,008,615 + dacB Synpcc7942_1934 0.72 -0.7 2,008,623 - dacB Synpcc7942_1934 0.73 +1.0 2,008,623 - dacB Synpcc7942_1934 0.73 -0.6 2,008,649 + dacB Synpcc7942_1934 0.75 +0.9 2,008,652 - dacB Synpcc7942_1934 0.75 -1.1 2,008,652 - dacB Synpcc7942_1934 0.75 +1.5 2,008,657 - dacB Synpcc7942_1934 0.75 +1.4 2,008,657 - dacB Synpcc7942_1934 0.75 -0.8 2,008,707 - dacB Synpcc7942_1934 0.79 +0.1 2,008,717 - dacB Synpcc7942_1934 0.80 +0.5 2,008,733 - dacB Synpcc7942_1934 0.81 +1.1 2,008,733 - dacB Synpcc7942_1934 0.81 -1.2 2,008,735 + dacB Synpcc7942_1934 0.81 +1.0 2,008,740 + dacB Synpcc7942_1934 0.81 +0.7 2,008,748 - dacB Synpcc7942_1934 0.82 +1.4 2,008,801 - dacB Synpcc7942_1934 0.86 +0.9 2,008,811 + dacB Synpcc7942_1934 0.87 +0.3 2,008,842 + dacB Synpcc7942_1934 0.89 -2.6 2,008,852 + dacB Synpcc7942_1934 0.90 -0.1 2,009,004 - +1.0 2,009,266 - +0.9 2,009,283 - +5.6 2,009,283 - +1.8 2,009,304 + +4.3 2,009,304 + +4.8 2,009,312 - pilD Synpcc7942_1935 0.10 +4.5 2,009,338 + pilD Synpcc7942_1935 0.13 +4.2 2,009,346 - pilD Synpcc7942_1935 0.14 +3.8 2,009,346 - pilD Synpcc7942_1935 0.14 +4.9 2,009,346 - pilD Synpcc7942_1935 0.14 +5.2 2,009,346 - pilD Synpcc7942_1935 0.14 +4.7 2,009,347 - pilD Synpcc7942_1935 0.15 +5.3 2,009,347 - pilD Synpcc7942_1935 0.15 +4.6 2,009,360 + pilD Synpcc7942_1935 0.16 +3.9 2,009,368 - pilD Synpcc7942_1935 0.17 +4.4 2,009,385 + pilD Synpcc7942_1935 0.19 +5.1 2,009,395 + pilD Synpcc7942_1935 0.21 +4.5 2,009,395 + pilD Synpcc7942_1935 0.21 +6.0 2,009,403 - pilD Synpcc7942_1935 0.22 +4.0 2,009,403 - pilD Synpcc7942_1935 0.22 +4.1 2,009,403 - pilD Synpcc7942_1935 0.22 +4.7 2,009,403 - pilD Synpcc7942_1935 0.22 +6.0 2,009,432 + pilD Synpcc7942_1935 0.25 +3.3 2,009,432 + pilD Synpcc7942_1935 0.25 +5.4 2,009,440 - pilD Synpcc7942_1935 0.26 +4.4 2,009,516 + pilD Synpcc7942_1935 0.36 +4.8 2,009,519 - pilD Synpcc7942_1935 0.36 +5.4 2,009,522 - pilD Synpcc7942_1935 0.37 +5.0 2,009,557 + pilD Synpcc7942_1935 0.41 +4.7 2,009,565 - pilD Synpcc7942_1935 0.42 +4.4 2,009,565 - pilD Synpcc7942_1935 0.42 +5.2 2,009,567 + pilD Synpcc7942_1935 0.42 +4.2 2,009,570 - pilD Synpcc7942_1935 0.43 +4.6 2,009,575 - pilD Synpcc7942_1935 0.43 +5.7 2,009,608 + pilD Synpcc7942_1935 0.48 +5.1 2,009,608 + pilD Synpcc7942_1935 0.48 +4.9 2,009,614 + pilD Synpcc7942_1935 0.48 +4.1 2,009,616 - +5.6 2,009,625 + pilD Synpcc7942_1935 0.50 +5.1 2,009,646 + pilD Synpcc7942_1935 0.52 +2.8 2,009,649 - pilD Synpcc7942_1935 0.53 +4.4 2,009,652 + pilD Synpcc7942_1935 0.53 +5.0 2,009,659 + pilD Synpcc7942_1935 0.54 +5.8 2,009,667 + pilD Synpcc7942_1935 0.55 +4.3 2,009,669 + pilD Synpcc7942_1935 0.55 +4.7 2,009,669 + pilD Synpcc7942_1935 0.55 +3.2 2,009,669 + pilD Synpcc7942_1935 0.55 +4.4 2,009,669 - pilD Synpcc7942_1935 0.55 +6.6 2,009,731 - pilD Synpcc7942_1935 0.63 +3.5 2,009,762 + pilD Synpcc7942_1935 0.67 +3.6 2,009,762 + pilD Synpcc7942_1935 0.67 +4.1 2,009,777 - pilD Synpcc7942_1935 0.69 +3.6 2,009,810 - pilD Synpcc7942_1935 0.73 +4.3 2,009,827 + pilD Synpcc7942_1935 0.75 +1.9 2,009,845 - pilD Synpcc7942_1935 0.78 +5.5 2,009,845 - pilD Synpcc7942_1935 0.78 +5.2 2,009,891 + pilD Synpcc7942_1935 0.84 +5.4 2,009,904 - pilD Synpcc7942_1935 0.85 +3.5 2,009,925 - pilD Synpcc7942_1935 0.88 +3.6 2,009,964 + +3.8 2,009,979 + +4.5 2,009,987 - +3.9 2,009,987 - +5.3
Or see this region's nucleotide sequence