Experiment: Vogels_fungal_media; pH 5.5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS04655 and HSERO_RS04660 are separated by 20 nucleotides HSERO_RS04660 and HSERO_RS04665 are separated by 61 nucleotides HSERO_RS04665 and HSERO_RS04670 are separated by 70 nucleotides
HSERO_RS04655: HSERO_RS04655 - hypothetical protein, at 1,014,058 to 1,015,275
_RS04655
HSERO_RS04660: HSERO_RS04660 - nitrile hydratase, at 1,015,296 to 1,015,955
_RS04660
HSERO_RS04665: HSERO_RS04665 - nitrile hydratase, at 1,016,017 to 1,016,616
_RS04665
HSERO_RS04670: HSERO_RS04670 - amidase, at 1,016,687 to 1,018,201
_RS04670
Position (kb)
1015
1016 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1014.605 kb on - strand, within HSERO_RS04655 at 1014.683 kb on + strand, within HSERO_RS04655 at 1014.686 kb on - strand, within HSERO_RS04655 at 1014.802 kb on + strand, within HSERO_RS04655 at 1014.807 kb on + strand, within HSERO_RS04655 at 1015.015 kb on - strand, within HSERO_RS04655 at 1015.020 kb on - strand, within HSERO_RS04655 at 1015.040 kb on + strand, within HSERO_RS04655 at 1015.188 kb on + strand at 1015.188 kb on + strand at 1015.188 kb on + strand at 1015.196 kb on - strand at 1015.207 kb on + strand at 1015.265 kb on + strand at 1015.278 kb on - strand at 1015.388 kb on - strand, within HSERO_RS04660 at 1015.424 kb on + strand, within HSERO_RS04660 at 1015.483 kb on + strand, within HSERO_RS04660 at 1015.499 kb on + strand, within HSERO_RS04660 at 1015.515 kb on + strand, within HSERO_RS04660 at 1015.633 kb on - strand, within HSERO_RS04660 at 1015.696 kb on - strand, within HSERO_RS04660 at 1015.801 kb on + strand, within HSERO_RS04660 at 1015.801 kb on + strand, within HSERO_RS04660 at 1015.847 kb on - strand, within HSERO_RS04660 at 1015.992 kb on + strand at 1015.997 kb on + strand at 1015.997 kb on + strand at 1016.002 kb on + strand at 1016.002 kb on + strand at 1016.024 kb on + strand at 1016.029 kb on + strand at 1016.057 kb on + strand at 1016.277 kb on - strand, within HSERO_RS04665 at 1016.438 kb on + strand, within HSERO_RS04665 at 1016.438 kb on + strand, within HSERO_RS04665 at 1016.580 kb on + strand at 1016.696 kb on + strand at 1016.730 kb on + strand at 1016.738 kb on - strand at 1016.797 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Vogels_fungal_media; pH 5.5 remove 1,014,605 - HSERO_RS04655 0.45 +0.7 1,014,683 + HSERO_RS04655 0.51 -1.7 1,014,686 - HSERO_RS04655 0.52 -0.4 1,014,802 + HSERO_RS04655 0.61 +0.5 1,014,807 + HSERO_RS04655 0.61 -0.0 1,015,015 - HSERO_RS04655 0.79 +0.3 1,015,020 - HSERO_RS04655 0.79 -0.7 1,015,040 + HSERO_RS04655 0.81 +0.1 1,015,188 + -0.0 1,015,188 + -0.0 1,015,188 + +1.1 1,015,196 - +0.8 1,015,207 + +2.4 1,015,265 + -3.4 1,015,278 - +0.1 1,015,388 - HSERO_RS04660 0.14 -1.7 1,015,424 + HSERO_RS04660 0.19 -1.0 1,015,483 + HSERO_RS04660 0.28 +0.7 1,015,499 + HSERO_RS04660 0.31 -0.3 1,015,515 + HSERO_RS04660 0.33 -1.5 1,015,633 - HSERO_RS04660 0.51 -0.5 1,015,696 - HSERO_RS04660 0.61 -0.5 1,015,801 + HSERO_RS04660 0.77 -0.3 1,015,801 + HSERO_RS04660 0.77 -0.5 1,015,847 - HSERO_RS04660 0.83 -0.4 1,015,992 + +0.3 1,015,997 + -0.0 1,015,997 + +1.3 1,016,002 + +0.1 1,016,002 + -0.7 1,016,024 + +1.2 1,016,029 + -0.1 1,016,057 + -0.5 1,016,277 - HSERO_RS04665 0.43 -2.2 1,016,438 + HSERO_RS04665 0.70 +0.7 1,016,438 + HSERO_RS04665 0.70 -0.1 1,016,580 + -1.0 1,016,696 + +0.2 1,016,730 + -3.1 1,016,738 - +0.2 1,016,797 - +0.4
Or see this region's nucleotide sequence