Experiment: D-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c04050 and PGA1_c04060 are separated by 122 nucleotides PGA1_c04060 and PGA1_c04070 overlap by 1 nucleotides PGA1_c04070 and PGA1_c04080 are separated by 73 nucleotides
PGA1_c04050: PGA1_c04050 - phenylacetic acid degradation protein PaaD, at 390,853 to 391,326
_c04050
PGA1_c04060: PGA1_c04060 - phenylacetic acid degradation protein PaaC, at 391,449 to 392,222
_c04060
PGA1_c04070: PGA1_c04070 - phenylacetic acid degradation protein PaaB, at 392,222 to 392,506
_c04070
PGA1_c04080: PGA1_c04080 - phenylacetic acid degradation protein PaaA, at 392,580 to 393,557
_c04080
Position (kb)
392
393 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 391.225 kb on + strand, within PGA1_c04050 at 391.261 kb on + strand, within PGA1_c04050 at 391.280 kb on - strand at 391.379 kb on - strand at 391.380 kb on - strand at 391.380 kb on - strand at 391.380 kb on - strand at 391.440 kb on + strand at 391.493 kb on + strand at 391.494 kb on + strand at 391.580 kb on - strand, within PGA1_c04060 at 391.592 kb on + strand, within PGA1_c04060 at 391.642 kb on + strand, within PGA1_c04060 at 391.692 kb on + strand, within PGA1_c04060 at 391.719 kb on + strand, within PGA1_c04060 at 391.767 kb on - strand, within PGA1_c04060 at 391.808 kb on - strand, within PGA1_c04060 at 391.858 kb on - strand, within PGA1_c04060 at 391.866 kb on - strand, within PGA1_c04060 at 392.028 kb on - strand, within PGA1_c04060 at 392.028 kb on - strand, within PGA1_c04060 at 392.028 kb on - strand, within PGA1_c04060 at 392.073 kb on - strand, within PGA1_c04060 at 392.073 kb on - strand, within PGA1_c04060 at 392.080 kb on + strand, within PGA1_c04060 at 392.080 kb on + strand, within PGA1_c04060 at 392.080 kb on + strand, within PGA1_c04060 at 392.080 kb on + strand, within PGA1_c04060 at 392.080 kb on + strand, within PGA1_c04060 at 392.080 kb on + strand, within PGA1_c04060 at 392.088 kb on - strand, within PGA1_c04060 at 392.088 kb on - strand, within PGA1_c04060 at 392.088 kb on - strand, within PGA1_c04060 at 392.088 kb on - strand, within PGA1_c04060 at 392.088 kb on - strand, within PGA1_c04060 at 392.121 kb on + strand, within PGA1_c04060 at 392.151 kb on + strand at 392.178 kb on - strand at 392.217 kb on + strand at 392.217 kb on + strand at 392.268 kb on + strand, within PGA1_c04070 at 392.275 kb on + strand, within PGA1_c04070 at 392.276 kb on - strand, within PGA1_c04070 at 392.282 kb on - strand, within PGA1_c04070 at 392.283 kb on - strand, within PGA1_c04070 at 392.329 kb on - strand, within PGA1_c04070 at 392.378 kb on + strand, within PGA1_c04070 at 392.386 kb on + strand, within PGA1_c04070 at 392.433 kb on + strand, within PGA1_c04070 at 392.439 kb on + strand, within PGA1_c04070 at 392.441 kb on - strand, within PGA1_c04070 at 392.457 kb on - strand, within PGA1_c04070 at 392.457 kb on - strand, within PGA1_c04070 at 392.522 kb on + strand at 392.530 kb on - strand at 392.537 kb on + strand at 392.577 kb on + strand at 392.577 kb on + strand at 392.580 kb on - strand at 392.656 kb on + strand at 392.663 kb on - strand at 392.664 kb on - strand at 392.675 kb on + strand at 392.677 kb on + strand at 392.685 kb on - strand, within PGA1_c04080 at 392.701 kb on + strand, within PGA1_c04080 at 392.715 kb on - strand, within PGA1_c04080 at 392.804 kb on + strand, within PGA1_c04080 at 392.810 kb on + strand, within PGA1_c04080 at 392.852 kb on - strand, within PGA1_c04080 at 392.874 kb on - strand, within PGA1_c04080 at 392.902 kb on + strand, within PGA1_c04080 at 392.924 kb on - strand, within PGA1_c04080 at 393.008 kb on - strand, within PGA1_c04080 at 393.025 kb on + strand, within PGA1_c04080 at 393.096 kb on - strand, within PGA1_c04080 at 393.136 kb on + strand, within PGA1_c04080 at 393.169 kb on + strand, within PGA1_c04080 at 393.262 kb on - strand, within PGA1_c04080 at 393.271 kb on + strand, within PGA1_c04080 at 393.271 kb on + strand, within PGA1_c04080 at 393.271 kb on + strand, within PGA1_c04080 at 393.271 kb on + strand, within PGA1_c04080 at 393.279 kb on - strand, within PGA1_c04080 at 393.281 kb on + strand, within PGA1_c04080 at 393.282 kb on + strand, within PGA1_c04080 at 393.289 kb on - strand, within PGA1_c04080 at 393.298 kb on + strand, within PGA1_c04080 at 393.306 kb on - strand, within PGA1_c04080 at 393.306 kb on - strand, within PGA1_c04080 at 393.366 kb on + strand, within PGA1_c04080 at 393.375 kb on - strand, within PGA1_c04080 at 393.420 kb on + strand, within PGA1_c04080 at 393.420 kb on + strand, within PGA1_c04080 at 393.468 kb on + strand at 393.476 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine (N) remove 391,225 + PGA1_c04050 0.78 -0.1 391,261 + PGA1_c04050 0.86 +0.2 391,280 - -0.2 391,379 - +0.2 391,380 - +0.7 391,380 - +1.8 391,380 - +1.5 391,440 + +0.2 391,493 + -0.3 391,494 + +0.4 391,580 - PGA1_c04060 0.17 -0.1 391,592 + PGA1_c04060 0.18 -0.3 391,642 + PGA1_c04060 0.25 -1.4 391,692 + PGA1_c04060 0.31 -0.6 391,719 + PGA1_c04060 0.35 +0.9 391,767 - PGA1_c04060 0.41 +0.8 391,808 - PGA1_c04060 0.46 +1.0 391,858 - PGA1_c04060 0.53 +0.4 391,866 - PGA1_c04060 0.54 +0.7 392,028 - PGA1_c04060 0.75 -0.4 392,028 - PGA1_c04060 0.75 -0.6 392,028 - PGA1_c04060 0.75 +0.5 392,073 - PGA1_c04060 0.81 +0.8 392,073 - PGA1_c04060 0.81 +0.5 392,080 + PGA1_c04060 0.82 +1.8 392,080 + PGA1_c04060 0.82 +0.6 392,080 + PGA1_c04060 0.82 +0.8 392,080 + PGA1_c04060 0.82 -0.5 392,080 + PGA1_c04060 0.82 +0.4 392,080 + PGA1_c04060 0.82 +0.6 392,088 - PGA1_c04060 0.83 +0.1 392,088 - PGA1_c04060 0.83 +0.2 392,088 - PGA1_c04060 0.83 +0.1 392,088 - PGA1_c04060 0.83 +0.3 392,088 - PGA1_c04060 0.83 +0.8 392,121 + PGA1_c04060 0.87 -0.1 392,151 + +1.0 392,178 - -2.6 392,217 + +0.1 392,217 + -0.0 392,268 + PGA1_c04070 0.16 +0.2 392,275 + PGA1_c04070 0.19 +0.0 392,276 - PGA1_c04070 0.19 +0.4 392,282 - PGA1_c04070 0.21 -0.4 392,283 - PGA1_c04070 0.21 +0.7 392,329 - PGA1_c04070 0.38 +0.9 392,378 + PGA1_c04070 0.55 +1.1 392,386 + PGA1_c04070 0.58 -0.2 392,433 + PGA1_c04070 0.74 +0.5 392,439 + PGA1_c04070 0.76 +0.1 392,441 - PGA1_c04070 0.77 -0.3 392,457 - PGA1_c04070 0.82 +0.8 392,457 - PGA1_c04070 0.82 +0.8 392,522 + -0.2 392,530 - +0.0 392,537 + +0.7 392,577 + +0.4 392,577 + +0.4 392,580 - +1.0 392,656 + +0.3 392,663 - +0.5 392,664 - +0.1 392,675 + -0.8 392,677 + +0.9 392,685 - PGA1_c04080 0.11 +0.5 392,701 + PGA1_c04080 0.12 +0.9 392,715 - PGA1_c04080 0.14 +0.1 392,804 + PGA1_c04080 0.23 -0.1 392,810 + PGA1_c04080 0.24 +1.2 392,852 - PGA1_c04080 0.28 -0.3 392,874 - PGA1_c04080 0.30 +2.1 392,902 + PGA1_c04080 0.33 +0.5 392,924 - PGA1_c04080 0.35 +0.2 393,008 - PGA1_c04080 0.44 -1.0 393,025 + PGA1_c04080 0.46 -0.4 393,096 - PGA1_c04080 0.53 +0.2 393,136 + PGA1_c04080 0.57 -0.4 393,169 + PGA1_c04080 0.60 -0.0 393,262 - PGA1_c04080 0.70 +0.3 393,271 + PGA1_c04080 0.71 +1.4 393,271 + PGA1_c04080 0.71 -0.1 393,271 + PGA1_c04080 0.71 +0.1 393,271 + PGA1_c04080 0.71 +0.5 393,279 - PGA1_c04080 0.71 +1.1 393,281 + PGA1_c04080 0.72 +0.7 393,282 + PGA1_c04080 0.72 +1.2 393,289 - PGA1_c04080 0.72 +0.1 393,298 + PGA1_c04080 0.73 -0.2 393,306 - PGA1_c04080 0.74 -0.2 393,306 - PGA1_c04080 0.74 +0.6 393,366 + PGA1_c04080 0.80 +0.0 393,375 - PGA1_c04080 0.81 +0.3 393,420 + PGA1_c04080 0.86 +0.4 393,420 + PGA1_c04080 0.86 +0.7 393,468 + +2.3 393,476 - -0.0
Or see this region's nucleotide sequence