Experiment: D-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_262p01100 and PGA1_262p01110 are separated by 125 nucleotides PGA1_262p01110 and PGA1_262p01120 are separated by 78 nucleotides PGA1_262p01120 and PGA1_262p01130 are separated by 61 nucleotides
PGA1_262p01100: PGA1_262p01100 - transposase, at 118,688 to 120,277
_262p01100
PGA1_262p01110: PGA1_262p01110 - hypothetical protein, at 120,403 to 120,828
_262p01110
PGA1_262p01120: PGA1_262p01120 - hypothetical protein, at 120,907 to 121,305
_262p01120
PGA1_262p01130: PGA1_262p01130 - hypothetical protein, at 121,367 to 122,032
_262p01130
Position (kb) 120
121 Strain fitness (log2 ratio) -1
0
1
2 at 120.400 kb on + strand at 120.521 kb on + strand, within PGA1_262p01110 at 120.539 kb on + strand, within PGA1_262p01110 at 120.559 kb on + strand, within PGA1_262p01110 at 120.567 kb on - strand, within PGA1_262p01110 at 120.567 kb on - strand, within PGA1_262p01110 at 120.618 kb on - strand, within PGA1_262p01110 at 120.650 kb on + strand, within PGA1_262p01110 at 120.658 kb on - strand, within PGA1_262p01110 at 120.660 kb on + strand, within PGA1_262p01110 at 120.667 kb on + strand, within PGA1_262p01110 at 120.764 kb on + strand, within PGA1_262p01110 at 120.889 kb on - strand at 120.913 kb on - strand at 121.021 kb on + strand, within PGA1_262p01120 at 121.029 kb on - strand, within PGA1_262p01120 at 121.093 kb on - strand, within PGA1_262p01120 at 121.266 kb on + strand at 121.351 kb on + strand at 121.446 kb on + strand, within PGA1_262p01130 at 121.584 kb on + strand, within PGA1_262p01130 at 121.592 kb on - strand, within PGA1_262p01130 at 121.765 kb on + strand, within PGA1_262p01130 at 121.773 kb on - strand, within PGA1_262p01130
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine (N) remove 120,400 + +0.2 120,521 + PGA1_262p01110 0.28 +0.4 120,539 + PGA1_262p01110 0.32 +2.0 120,559 + PGA1_262p01110 0.37 -0.6 120,567 - PGA1_262p01110 0.38 +0.2 120,567 - PGA1_262p01110 0.38 +0.4 120,618 - PGA1_262p01110 0.50 +0.5 120,650 + PGA1_262p01110 0.58 +0.5 120,658 - PGA1_262p01110 0.60 +0.3 120,660 + PGA1_262p01110 0.60 +0.7 120,667 + PGA1_262p01110 0.62 -0.3 120,764 + PGA1_262p01110 0.85 +0.2 120,889 - -0.4 120,913 - -0.0 121,021 + PGA1_262p01120 0.29 -0.0 121,029 - PGA1_262p01120 0.31 +0.1 121,093 - PGA1_262p01120 0.47 -1.4 121,266 + +0.7 121,351 + +0.2 121,446 + PGA1_262p01130 0.12 +0.4 121,584 + PGA1_262p01130 0.33 +0.5 121,592 - PGA1_262p01130 0.34 -0.2 121,765 + PGA1_262p01130 0.60 -0.1 121,773 - PGA1_262p01130 0.61 +0.3
Or see this region's nucleotide sequence