Experiment: D-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_262p00780 and PGA1_262p00790 are separated by 97 nucleotides PGA1_262p00790 and PGA1_262p00800 are separated by 103 nucleotides PGA1_262p00800 and PGA1_262p00810 are separated by 34 nucleotides PGA1_262p00810 and PGA1_262p00820 are separated by 79 nucleotides
PGA1_262p00780: PGA1_262p00780 - NAD dependent epimerase/dehydratase, at 87,330 to 88,439
_262p00780
PGA1_262p00790: PGA1_262p00790 - hypothetical protein, at 88,537 to 88,668
_262p00790
PGA1_262p00800: PGA1_262p00800 - phenylacetic acid degradation protein PaaZ, at 88,772 to 90,904
_262p00800
PGA1_262p00810: PGA1_262p00810 - putative flavoprotein, HFCD family, at 90,939 to 91,508
_262p00810
PGA1_262p00820: PGA1_262p00820 - hypothetical protein, at 91,588 to 92,028
_262p00820
Position (kb) 88
89
90
91 Strain fitness (log2 ratio) -1
0
1 at 87.823 kb on + strand, within PGA1_262p00780 at 87.833 kb on + strand, within PGA1_262p00780 at 87.911 kb on + strand, within PGA1_262p00780 at 87.954 kb on + strand, within PGA1_262p00780 at 87.973 kb on + strand, within PGA1_262p00780 at 88.026 kb on - strand, within PGA1_262p00780 at 88.026 kb on - strand, within PGA1_262p00780 at 88.052 kb on + strand, within PGA1_262p00780 at 88.441 kb on + strand at 88.721 kb on + strand at 88.764 kb on + strand at 88.764 kb on + strand at 88.772 kb on - strand at 88.772 kb on - strand at 88.861 kb on - strand at 88.879 kb on + strand at 88.892 kb on - strand at 88.903 kb on - strand at 88.943 kb on + strand at 88.988 kb on + strand, within PGA1_262p00800 at 89.033 kb on + strand, within PGA1_262p00800 at 89.071 kb on + strand, within PGA1_262p00800 at 89.110 kb on + strand, within PGA1_262p00800 at 89.122 kb on + strand, within PGA1_262p00800 at 89.132 kb on + strand, within PGA1_262p00800 at 89.141 kb on + strand, within PGA1_262p00800 at 89.159 kb on + strand, within PGA1_262p00800 at 89.232 kb on + strand, within PGA1_262p00800 at 89.240 kb on - strand, within PGA1_262p00800 at 89.240 kb on - strand, within PGA1_262p00800 at 89.438 kb on + strand, within PGA1_262p00800 at 89.485 kb on + strand, within PGA1_262p00800 at 89.612 kb on - strand, within PGA1_262p00800 at 89.678 kb on - strand, within PGA1_262p00800 at 89.731 kb on + strand, within PGA1_262p00800 at 89.840 kb on + strand, within PGA1_262p00800 at 89.888 kb on + strand, within PGA1_262p00800 at 89.947 kb on - strand, within PGA1_262p00800 at 90.038 kb on + strand, within PGA1_262p00800 at 90.057 kb on + strand, within PGA1_262p00800 at 90.076 kb on + strand, within PGA1_262p00800 at 90.194 kb on - strand, within PGA1_262p00800 at 90.209 kb on + strand, within PGA1_262p00800 at 90.224 kb on - strand, within PGA1_262p00800 at 90.226 kb on + strand, within PGA1_262p00800 at 90.234 kb on - strand, within PGA1_262p00800 at 90.234 kb on - strand, within PGA1_262p00800 at 90.345 kb on - strand, within PGA1_262p00800 at 90.403 kb on + strand, within PGA1_262p00800 at 90.533 kb on + strand, within PGA1_262p00800 at 90.548 kb on + strand, within PGA1_262p00800 at 90.548 kb on + strand, within PGA1_262p00800 at 90.556 kb on - strand, within PGA1_262p00800 at 90.596 kb on + strand, within PGA1_262p00800 at 90.596 kb on + strand, within PGA1_262p00800 at 90.599 kb on - strand, within PGA1_262p00800 at 90.599 kb on - strand, within PGA1_262p00800 at 90.699 kb on + strand at 90.745 kb on + strand at 90.798 kb on - strand at 90.832 kb on + strand at 90.840 kb on - strand at 90.949 kb on - strand at 90.996 kb on - strand, within PGA1_262p00810 at 91.042 kb on + strand, within PGA1_262p00810 at 91.042 kb on + strand, within PGA1_262p00810 at 91.050 kb on - strand, within PGA1_262p00810 at 91.061 kb on + strand, within PGA1_262p00810 at 91.069 kb on - strand, within PGA1_262p00810 at 91.069 kb on - strand, within PGA1_262p00810 at 91.147 kb on - strand, within PGA1_262p00810 at 91.265 kb on - strand, within PGA1_262p00810 at 91.293 kb on + strand, within PGA1_262p00810 at 91.375 kb on + strand, within PGA1_262p00810 at 91.569 kb on - strand at 91.585 kb on + strand at 91.791 kb on - strand, within PGA1_262p00820 at 91.871 kb on + strand, within PGA1_262p00820
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine (N) remove 87,823 + PGA1_262p00780 0.44 +0.1 87,833 + PGA1_262p00780 0.45 -0.2 87,911 + PGA1_262p00780 0.52 -0.2 87,954 + PGA1_262p00780 0.56 +0.5 87,973 + PGA1_262p00780 0.58 +0.2 88,026 - PGA1_262p00780 0.63 +0.3 88,026 - PGA1_262p00780 0.63 -0.8 88,052 + PGA1_262p00780 0.65 +0.6 88,441 + +0.2 88,721 + +1.1 88,764 + -0.2 88,764 + +0.3 88,772 - -0.0 88,772 - +1.6 88,861 - +0.4 88,879 + +0.2 88,892 - +0.9 88,903 - -0.2 88,943 + +0.8 88,988 + PGA1_262p00800 0.10 +0.2 89,033 + PGA1_262p00800 0.12 +0.1 89,071 + PGA1_262p00800 0.14 +0.0 89,110 + PGA1_262p00800 0.16 +0.3 89,122 + PGA1_262p00800 0.16 -0.1 89,132 + PGA1_262p00800 0.17 +1.2 89,141 + PGA1_262p00800 0.17 +0.1 89,159 + PGA1_262p00800 0.18 +0.4 89,232 + PGA1_262p00800 0.22 -0.2 89,240 - PGA1_262p00800 0.22 -0.3 89,240 - PGA1_262p00800 0.22 +0.3 89,438 + PGA1_262p00800 0.31 -0.1 89,485 + PGA1_262p00800 0.33 +0.6 89,612 - PGA1_262p00800 0.39 +0.4 89,678 - PGA1_262p00800 0.42 +0.1 89,731 + PGA1_262p00800 0.45 +0.2 89,840 + PGA1_262p00800 0.50 +0.3 89,888 + PGA1_262p00800 0.52 +0.3 89,947 - PGA1_262p00800 0.55 -0.2 90,038 + PGA1_262p00800 0.59 +0.7 90,057 + PGA1_262p00800 0.60 +0.4 90,076 + PGA1_262p00800 0.61 +0.8 90,194 - PGA1_262p00800 0.67 +0.8 90,209 + PGA1_262p00800 0.67 -0.3 90,224 - PGA1_262p00800 0.68 +1.0 90,226 + PGA1_262p00800 0.68 +0.6 90,234 - PGA1_262p00800 0.69 +0.4 90,234 - PGA1_262p00800 0.69 -0.0 90,345 - PGA1_262p00800 0.74 -0.4 90,403 + PGA1_262p00800 0.76 +0.0 90,533 + PGA1_262p00800 0.83 +0.6 90,548 + PGA1_262p00800 0.83 +0.9 90,548 + PGA1_262p00800 0.83 -0.5 90,556 - PGA1_262p00800 0.84 +0.2 90,596 + PGA1_262p00800 0.86 +0.2 90,596 + PGA1_262p00800 0.86 +0.8 90,599 - PGA1_262p00800 0.86 +0.0 90,599 - PGA1_262p00800 0.86 +0.2 90,699 + -0.3 90,745 + +0.0 90,798 - +1.0 90,832 + +0.0 90,840 - -0.3 90,949 - +0.1 90,996 - PGA1_262p00810 0.10 -0.0 91,042 + PGA1_262p00810 0.18 -0.2 91,042 + PGA1_262p00810 0.18 +0.5 91,050 - PGA1_262p00810 0.19 -1.1 91,061 + PGA1_262p00810 0.21 -0.7 91,069 - PGA1_262p00810 0.23 +0.2 91,069 - PGA1_262p00810 0.23 +0.4 91,147 - PGA1_262p00810 0.36 +0.7 91,265 - PGA1_262p00810 0.57 +0.2 91,293 + PGA1_262p00810 0.62 -0.9 91,375 + PGA1_262p00810 0.76 +0.8 91,569 - -0.5 91,585 + -0.1 91,791 - PGA1_262p00820 0.46 -0.2 91,871 + PGA1_262p00820 0.64 -0.0
Or see this region's nucleotide sequence