Experiment: D-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c30470 and PGA1_c30480 are separated by 159 nucleotides PGA1_c30480 and PGA1_c30490 are separated by 315 nucleotides
PGA1_c30470: PGA1_c30470 - mandelate racemase / muconate lactonizing enzyme, at 3,182,341 to 3,183,306
_c30470
PGA1_c30480: PGA1_c30480 - Uncharacterized conserved protein, at 3,183,466 to 3,184,467
_c30480
PGA1_c30490: PGA1_c30490 - Malyl-CoA lyase (EC 4.1.3.24) (from data) , at 3,184,783 to 3,185,745
_c30490
Position (kb)
3183
3184
3185 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3182.487 kb on + strand, within PGA1_c30470 at 3182.517 kb on + strand, within PGA1_c30470 at 3182.576 kb on + strand, within PGA1_c30470 at 3182.662 kb on + strand, within PGA1_c30470 at 3182.662 kb on + strand, within PGA1_c30470 at 3182.670 kb on - strand, within PGA1_c30470 at 3182.773 kb on + strand, within PGA1_c30470 at 3182.928 kb on + strand, within PGA1_c30470 at 3183.085 kb on + strand, within PGA1_c30470 at 3183.127 kb on - strand, within PGA1_c30470 at 3183.150 kb on - strand, within PGA1_c30470 at 3183.293 kb on + strand at 3183.306 kb on - strand at 3183.311 kb on - strand at 3183.313 kb on + strand at 3183.323 kb on - strand at 3183.334 kb on - strand at 3183.356 kb on + strand at 3183.364 kb on - strand at 3183.364 kb on - strand at 3183.471 kb on - strand at 3183.496 kb on - strand at 3183.541 kb on + strand at 3183.541 kb on + strand at 3183.541 kb on + strand at 3183.581 kb on + strand, within PGA1_c30480 at 3183.588 kb on - strand, within PGA1_c30480 at 3183.593 kb on - strand, within PGA1_c30480 at 3183.601 kb on - strand, within PGA1_c30480 at 3183.669 kb on + strand, within PGA1_c30480 at 3183.684 kb on + strand, within PGA1_c30480 at 3183.685 kb on - strand, within PGA1_c30480 at 3183.731 kb on + strand, within PGA1_c30480 at 3183.743 kb on - strand, within PGA1_c30480 at 3183.766 kb on - strand, within PGA1_c30480 at 3183.790 kb on + strand, within PGA1_c30480 at 3183.808 kb on + strand, within PGA1_c30480 at 3183.808 kb on + strand, within PGA1_c30480 at 3183.816 kb on - strand, within PGA1_c30480 at 3183.825 kb on + strand, within PGA1_c30480 at 3183.911 kb on - strand, within PGA1_c30480 at 3183.922 kb on + strand, within PGA1_c30480 at 3183.925 kb on + strand, within PGA1_c30480 at 3183.934 kb on + strand, within PGA1_c30480 at 3183.942 kb on - strand, within PGA1_c30480 at 3183.942 kb on - strand, within PGA1_c30480 at 3183.943 kb on - strand, within PGA1_c30480 at 3183.994 kb on + strand, within PGA1_c30480 at 3184.045 kb on - strand, within PGA1_c30480 at 3184.087 kb on + strand, within PGA1_c30480 at 3184.100 kb on - strand, within PGA1_c30480 at 3184.129 kb on + strand, within PGA1_c30480 at 3184.129 kb on + strand, within PGA1_c30480 at 3184.129 kb on + strand, within PGA1_c30480 at 3184.137 kb on - strand, within PGA1_c30480 at 3184.137 kb on - strand, within PGA1_c30480 at 3184.137 kb on - strand, within PGA1_c30480 at 3184.137 kb on - strand, within PGA1_c30480 at 3184.137 kb on - strand, within PGA1_c30480 at 3184.137 kb on - strand, within PGA1_c30480 at 3184.137 kb on - strand, within PGA1_c30480 at 3184.137 kb on - strand, within PGA1_c30480 at 3184.144 kb on + strand, within PGA1_c30480 at 3184.154 kb on + strand, within PGA1_c30480 at 3184.177 kb on - strand, within PGA1_c30480 at 3184.204 kb on + strand, within PGA1_c30480 at 3184.212 kb on - strand, within PGA1_c30480 at 3184.241 kb on - strand, within PGA1_c30480 at 3184.269 kb on - strand, within PGA1_c30480 at 3184.303 kb on - strand, within PGA1_c30480 at 3184.303 kb on - strand, within PGA1_c30480 at 3184.303 kb on - strand, within PGA1_c30480 at 3184.303 kb on - strand, within PGA1_c30480 at 3184.309 kb on - strand, within PGA1_c30480 at 3184.365 kb on - strand, within PGA1_c30480 at 3184.473 kb on + strand at 3184.489 kb on + strand at 3184.505 kb on + strand at 3184.511 kb on + strand at 3184.513 kb on - strand at 3184.513 kb on - strand at 3184.533 kb on + strand at 3184.673 kb on + strand at 3184.834 kb on - strand at 3184.836 kb on + strand at 3184.836 kb on + strand at 3184.846 kb on + strand at 3184.863 kb on + strand at 3184.886 kb on + strand, within PGA1_c30490 at 3184.891 kb on + strand, within PGA1_c30490 at 3184.896 kb on + strand, within PGA1_c30490 at 3184.904 kb on - strand, within PGA1_c30490 at 3184.939 kb on + strand, within PGA1_c30490 at 3184.939 kb on + strand, within PGA1_c30490 at 3184.939 kb on + strand, within PGA1_c30490 at 3184.947 kb on - strand, within PGA1_c30490 at 3184.949 kb on + strand, within PGA1_c30490 at 3184.953 kb on - strand, within PGA1_c30490 at 3184.953 kb on - strand, within PGA1_c30490 at 3184.957 kb on - strand, within PGA1_c30490 at 3184.966 kb on + strand, within PGA1_c30490 at 3184.966 kb on + strand, within PGA1_c30490 at 3184.968 kb on + strand, within PGA1_c30490 at 3184.968 kb on + strand, within PGA1_c30490 at 3184.974 kb on - strand, within PGA1_c30490 at 3184.974 kb on - strand, within PGA1_c30490 at 3184.976 kb on + strand, within PGA1_c30490 at 3184.976 kb on - strand, within PGA1_c30490 at 3184.976 kb on - strand, within PGA1_c30490 at 3185.010 kb on + strand, within PGA1_c30490 at 3185.022 kb on + strand, within PGA1_c30490 at 3185.022 kb on + strand, within PGA1_c30490 at 3185.028 kb on + strand, within PGA1_c30490 at 3185.030 kb on - strand, within PGA1_c30490 at 3185.030 kb on - strand, within PGA1_c30490 at 3185.105 kb on - strand, within PGA1_c30490 at 3185.129 kb on - strand, within PGA1_c30490 at 3185.227 kb on + strand, within PGA1_c30490 at 3185.227 kb on + strand, within PGA1_c30490 at 3185.234 kb on - strand, within PGA1_c30490 at 3185.270 kb on - strand, within PGA1_c30490 at 3185.278 kb on + strand, within PGA1_c30490 at 3185.279 kb on - strand, within PGA1_c30490 at 3185.279 kb on - strand, within PGA1_c30490 at 3185.293 kb on + strand, within PGA1_c30490 at 3185.342 kb on + strand, within PGA1_c30490 at 3185.342 kb on + strand, within PGA1_c30490 at 3185.350 kb on - strand, within PGA1_c30490 at 3185.350 kb on - strand, within PGA1_c30490 at 3185.350 kb on - strand, within PGA1_c30490 at 3185.350 kb on - strand, within PGA1_c30490 at 3185.350 kb on - strand, within PGA1_c30490 at 3185.380 kb on - strand, within PGA1_c30490
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine (N) remove 3,182,487 + PGA1_c30470 0.15 +0.2 3,182,517 + PGA1_c30470 0.18 -0.9 3,182,576 + PGA1_c30470 0.24 -1.8 3,182,662 + PGA1_c30470 0.33 -0.9 3,182,662 + PGA1_c30470 0.33 -1.3 3,182,670 - PGA1_c30470 0.34 -2.6 3,182,773 + PGA1_c30470 0.45 -2.9 3,182,928 + PGA1_c30470 0.61 -2.2 3,183,085 + PGA1_c30470 0.77 -2.1 3,183,127 - PGA1_c30470 0.81 -0.2 3,183,150 - PGA1_c30470 0.84 -2.9 3,183,293 + -0.0 3,183,306 - -0.6 3,183,311 - -2.9 3,183,313 + -0.6 3,183,323 - -2.6 3,183,334 - -1.7 3,183,356 + -2.1 3,183,364 - -0.2 3,183,364 - +0.9 3,183,471 - +0.3 3,183,496 - -1.9 3,183,541 + -1.0 3,183,541 + -1.9 3,183,541 + -0.9 3,183,581 + PGA1_c30480 0.11 -1.3 3,183,588 - PGA1_c30480 0.12 -1.0 3,183,593 - PGA1_c30480 0.13 -1.7 3,183,601 - PGA1_c30480 0.13 +0.8 3,183,669 + PGA1_c30480 0.20 -1.6 3,183,684 + PGA1_c30480 0.22 -0.2 3,183,685 - PGA1_c30480 0.22 -2.6 3,183,731 + PGA1_c30480 0.26 -1.8 3,183,743 - PGA1_c30480 0.28 -0.1 3,183,766 - PGA1_c30480 0.30 -0.5 3,183,790 + PGA1_c30480 0.32 -1.1 3,183,808 + PGA1_c30480 0.34 -0.8 3,183,808 + PGA1_c30480 0.34 -0.2 3,183,816 - PGA1_c30480 0.35 -1.9 3,183,825 + PGA1_c30480 0.36 -1.7 3,183,911 - PGA1_c30480 0.44 -0.6 3,183,922 + PGA1_c30480 0.46 -1.5 3,183,925 + PGA1_c30480 0.46 -1.4 3,183,934 + PGA1_c30480 0.47 -0.7 3,183,942 - PGA1_c30480 0.48 -1.4 3,183,942 - PGA1_c30480 0.48 +0.1 3,183,943 - PGA1_c30480 0.48 -1.5 3,183,994 + PGA1_c30480 0.53 -1.9 3,184,045 - PGA1_c30480 0.58 -1.1 3,184,087 + PGA1_c30480 0.62 -0.2 3,184,100 - PGA1_c30480 0.63 -1.3 3,184,129 + PGA1_c30480 0.66 -2.6 3,184,129 + PGA1_c30480 0.66 -0.9 3,184,129 + PGA1_c30480 0.66 -0.8 3,184,137 - PGA1_c30480 0.67 +0.3 3,184,137 - PGA1_c30480 0.67 +0.5 3,184,137 - PGA1_c30480 0.67 -0.9 3,184,137 - PGA1_c30480 0.67 -0.8 3,184,137 - PGA1_c30480 0.67 -0.4 3,184,137 - PGA1_c30480 0.67 +0.0 3,184,137 - PGA1_c30480 0.67 -2.1 3,184,137 - PGA1_c30480 0.67 -0.5 3,184,144 + PGA1_c30480 0.68 +0.3 3,184,154 + PGA1_c30480 0.69 -0.4 3,184,177 - PGA1_c30480 0.71 -2.0 3,184,204 + PGA1_c30480 0.74 -1.0 3,184,212 - PGA1_c30480 0.74 -1.9 3,184,241 - PGA1_c30480 0.77 -1.3 3,184,269 - PGA1_c30480 0.80 -0.7 3,184,303 - PGA1_c30480 0.84 +0.6 3,184,303 - PGA1_c30480 0.84 +0.2 3,184,303 - PGA1_c30480 0.84 -1.8 3,184,303 - PGA1_c30480 0.84 -0.1 3,184,309 - PGA1_c30480 0.84 +1.0 3,184,365 - PGA1_c30480 0.90 -2.3 3,184,473 + -0.8 3,184,489 + -1.2 3,184,505 + -1.4 3,184,511 + -0.6 3,184,513 - -1.4 3,184,513 - -0.7 3,184,533 + +0.4 3,184,673 + +0.0 3,184,834 - +0.4 3,184,836 + -0.3 3,184,836 + -0.3 3,184,846 + -0.9 3,184,863 + +0.6 3,184,886 + PGA1_c30490 0.11 -1.1 3,184,891 + PGA1_c30490 0.11 +1.8 3,184,896 + PGA1_c30490 0.12 +0.3 3,184,904 - PGA1_c30490 0.13 -0.8 3,184,939 + PGA1_c30490 0.16 +0.3 3,184,939 + PGA1_c30490 0.16 +0.3 3,184,939 + PGA1_c30490 0.16 +0.5 3,184,947 - PGA1_c30490 0.17 -2.0 3,184,949 + PGA1_c30490 0.17 -0.7 3,184,953 - PGA1_c30490 0.18 +0.8 3,184,953 - PGA1_c30490 0.18 +0.1 3,184,957 - PGA1_c30490 0.18 -0.4 3,184,966 + PGA1_c30490 0.19 -1.0 3,184,966 + PGA1_c30490 0.19 -0.0 3,184,968 + PGA1_c30490 0.19 -0.5 3,184,968 + PGA1_c30490 0.19 +0.1 3,184,974 - PGA1_c30490 0.20 +0.5 3,184,974 - PGA1_c30490 0.20 +1.8 3,184,976 + PGA1_c30490 0.20 +0.1 3,184,976 - PGA1_c30490 0.20 +0.4 3,184,976 - PGA1_c30490 0.20 +0.1 3,185,010 + PGA1_c30490 0.24 +0.4 3,185,022 + PGA1_c30490 0.25 +1.1 3,185,022 + PGA1_c30490 0.25 -0.1 3,185,028 + PGA1_c30490 0.25 +1.0 3,185,030 - PGA1_c30490 0.26 +0.1 3,185,030 - PGA1_c30490 0.26 +0.1 3,185,105 - PGA1_c30490 0.33 -0.4 3,185,129 - PGA1_c30490 0.36 -0.2 3,185,227 + PGA1_c30490 0.46 +0.5 3,185,227 + PGA1_c30490 0.46 -0.6 3,185,234 - PGA1_c30490 0.47 -0.0 3,185,270 - PGA1_c30490 0.51 -0.1 3,185,278 + PGA1_c30490 0.51 -0.2 3,185,279 - PGA1_c30490 0.52 -0.2 3,185,279 - PGA1_c30490 0.52 -0.0 3,185,293 + PGA1_c30490 0.53 +0.4 3,185,342 + PGA1_c30490 0.58 -0.3 3,185,342 + PGA1_c30490 0.58 -0.8 3,185,350 - PGA1_c30490 0.59 +1.0 3,185,350 - PGA1_c30490 0.59 -0.2 3,185,350 - PGA1_c30490 0.59 -0.3 3,185,350 - PGA1_c30490 0.59 -0.7 3,185,350 - PGA1_c30490 0.59 -1.6 3,185,380 - PGA1_c30490 0.62 +0.8
Or see this region's nucleotide sequence