Experiment: D-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c26600 and PGA1_c26610 are separated by 64 nucleotides PGA1_c26610 and PGA1_c26620 are separated by 33 nucleotides PGA1_c26620 and PGA1_c26630 overlap by 1 nucleotides PGA1_c26630 and PGA1_c26640 are separated by 42 nucleotides
PGA1_c26600: PGA1_c26600 - amino acid ABC transporter, permease protein, at 2,775,636 to 2,776,457
_c26600
PGA1_c26610: PGA1_c26610 - amino acid ABC transporter, substrate-binding protein, at 2,776,522 to 2,777,334
_c26610
PGA1_c26620: PGA1_c26620 - putative molybdopterin biosynthesis protein MoeB, at 2,777,368 to 2,778,504
_c26620
PGA1_c26630: PGA1_c26630 - deoxyuridine 5'-triphosphate nucleotidohydrolase Dut, at 2,778,504 to 2,778,959
_c26630
PGA1_c26640: PGA1_c26640 - coenzyme A biosynthesis bifunctional protein CoaBC, at 2,779,002 to 2,780,204
_c26640
Position (kb)
2777
2778
2779 Strain fitness (log2 ratio)
-1
0
1
2 at 2776.429 kb on - strand at 2776.436 kb on + strand at 2776.439 kb on - strand at 2776.450 kb on - strand at 2776.465 kb on - strand at 2776.504 kb on + strand at 2776.514 kb on + strand at 2776.522 kb on - strand at 2776.566 kb on + strand at 2776.577 kb on + strand at 2776.583 kb on + strand at 2776.583 kb on + strand at 2776.607 kb on - strand, within PGA1_c26610 at 2776.625 kb on + strand, within PGA1_c26610 at 2776.650 kb on - strand, within PGA1_c26610 at 2776.656 kb on + strand, within PGA1_c26610 at 2776.661 kb on + strand, within PGA1_c26610 at 2776.702 kb on + strand, within PGA1_c26610 at 2776.710 kb on - strand, within PGA1_c26610 at 2776.819 kb on + strand, within PGA1_c26610 at 2776.826 kb on - strand, within PGA1_c26610 at 2776.873 kb on - strand, within PGA1_c26610 at 2776.873 kb on - strand, within PGA1_c26610 at 2776.873 kb on - strand, within PGA1_c26610 at 2776.873 kb on - strand, within PGA1_c26610 at 2777.158 kb on - strand, within PGA1_c26610 at 2777.170 kb on - strand, within PGA1_c26610 at 2777.237 kb on - strand, within PGA1_c26610 at 2777.237 kb on - strand, within PGA1_c26610 at 2777.288 kb on - strand at 2777.341 kb on - strand at 2777.375 kb on - strand at 2777.413 kb on + strand at 2777.450 kb on + strand at 2777.451 kb on + strand at 2777.459 kb on - strand at 2777.459 kb on - strand at 2777.533 kb on + strand, within PGA1_c26620 at 2777.554 kb on + strand, within PGA1_c26620 at 2777.554 kb on + strand, within PGA1_c26620 at 2777.554 kb on + strand, within PGA1_c26620 at 2777.562 kb on - strand, within PGA1_c26620 at 2777.562 kb on - strand, within PGA1_c26620 at 2777.562 kb on - strand, within PGA1_c26620 at 2777.562 kb on - strand, within PGA1_c26620 at 2777.615 kb on - strand, within PGA1_c26620 at 2777.781 kb on + strand, within PGA1_c26620 at 2777.928 kb on + strand, within PGA1_c26620 at 2778.036 kb on + strand, within PGA1_c26620 at 2778.044 kb on - strand, within PGA1_c26620 at 2778.044 kb on - strand, within PGA1_c26620 at 2778.059 kb on - strand, within PGA1_c26620 at 2778.138 kb on + strand, within PGA1_c26620 at 2778.141 kb on - strand, within PGA1_c26620 at 2778.226 kb on - strand, within PGA1_c26620 at 2778.239 kb on - strand, within PGA1_c26620 at 2778.265 kb on - strand, within PGA1_c26620 at 2778.265 kb on - strand, within PGA1_c26620 at 2778.413 kb on + strand at 2778.449 kb on - strand at 2778.472 kb on + strand at 2778.510 kb on - strand at 2778.520 kb on - strand at 2778.598 kb on + strand, within PGA1_c26630 at 2778.606 kb on - strand, within PGA1_c26630 at 2778.606 kb on - strand, within PGA1_c26630 at 2778.662 kb on + strand, within PGA1_c26630 at 2778.775 kb on - strand, within PGA1_c26630 at 2778.775 kb on - strand, within PGA1_c26630 at 2778.775 kb on - strand, within PGA1_c26630 at 2778.810 kb on - strand, within PGA1_c26630 at 2778.833 kb on + strand, within PGA1_c26630 at 2778.835 kb on - strand, within PGA1_c26630 at 2778.869 kb on + strand, within PGA1_c26630 at 2778.911 kb on + strand, within PGA1_c26630 at 2778.911 kb on + strand, within PGA1_c26630 at 2778.932 kb on + strand at 2778.966 kb on + strand at 2778.966 kb on + strand at 2778.974 kb on - strand at 2779.008 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine (N) remove 2,776,429 - +1.2 2,776,436 + -0.1 2,776,439 - -0.7 2,776,450 - -0.8 2,776,465 - -0.4 2,776,504 + -0.4 2,776,514 + -0.6 2,776,522 - -0.0 2,776,566 + -0.6 2,776,577 + -0.3 2,776,583 + +0.5 2,776,583 + +1.4 2,776,607 - PGA1_c26610 0.10 -0.0 2,776,625 + PGA1_c26610 0.13 -1.3 2,776,650 - PGA1_c26610 0.16 +1.5 2,776,656 + PGA1_c26610 0.16 -0.7 2,776,661 + PGA1_c26610 0.17 -0.4 2,776,702 + PGA1_c26610 0.22 +0.2 2,776,710 - PGA1_c26610 0.23 -0.2 2,776,819 + PGA1_c26610 0.37 -0.2 2,776,826 - PGA1_c26610 0.37 +0.3 2,776,873 - PGA1_c26610 0.43 +0.3 2,776,873 - PGA1_c26610 0.43 -0.3 2,776,873 - PGA1_c26610 0.43 -1.0 2,776,873 - PGA1_c26610 0.43 -1.6 2,777,158 - PGA1_c26610 0.78 -0.2 2,777,170 - PGA1_c26610 0.80 -0.8 2,777,237 - PGA1_c26610 0.88 -0.5 2,777,237 - PGA1_c26610 0.88 -0.3 2,777,288 - +0.2 2,777,341 - +0.2 2,777,375 - +0.3 2,777,413 + -0.3 2,777,450 + +0.6 2,777,451 + +0.0 2,777,459 - +1.3 2,777,459 - -0.5 2,777,533 + PGA1_c26620 0.15 +2.3 2,777,554 + PGA1_c26620 0.16 +0.7 2,777,554 + PGA1_c26620 0.16 +0.4 2,777,554 + PGA1_c26620 0.16 -0.2 2,777,562 - PGA1_c26620 0.17 +0.7 2,777,562 - PGA1_c26620 0.17 +0.6 2,777,562 - PGA1_c26620 0.17 +0.2 2,777,562 - PGA1_c26620 0.17 +1.0 2,777,615 - PGA1_c26620 0.22 +1.1 2,777,781 + PGA1_c26620 0.36 +0.2 2,777,928 + PGA1_c26620 0.49 +0.7 2,778,036 + PGA1_c26620 0.59 +0.5 2,778,044 - PGA1_c26620 0.59 +0.5 2,778,044 - PGA1_c26620 0.59 +1.4 2,778,059 - PGA1_c26620 0.61 +0.1 2,778,138 + PGA1_c26620 0.68 -0.3 2,778,141 - PGA1_c26620 0.68 -0.1 2,778,226 - PGA1_c26620 0.75 +0.3 2,778,239 - PGA1_c26620 0.77 +0.4 2,778,265 - PGA1_c26620 0.79 +0.4 2,778,265 - PGA1_c26620 0.79 +0.0 2,778,413 + -0.3 2,778,449 - -0.8 2,778,472 + -0.3 2,778,510 - -0.5 2,778,520 - -0.4 2,778,598 + PGA1_c26630 0.21 +0.2 2,778,606 - PGA1_c26630 0.22 -1.3 2,778,606 - PGA1_c26630 0.22 +0.0 2,778,662 + PGA1_c26630 0.35 +0.0 2,778,775 - PGA1_c26630 0.59 +0.1 2,778,775 - PGA1_c26630 0.59 +0.6 2,778,775 - PGA1_c26630 0.59 -0.8 2,778,810 - PGA1_c26630 0.67 +0.9 2,778,833 + PGA1_c26630 0.72 -0.5 2,778,835 - PGA1_c26630 0.73 +2.1 2,778,869 + PGA1_c26630 0.80 +0.4 2,778,911 + PGA1_c26630 0.89 -0.0 2,778,911 + PGA1_c26630 0.89 -0.3 2,778,932 + -0.0 2,778,966 + +0.2 2,778,966 + +0.1 2,778,974 - -1.0 2,779,008 - +0.6
Or see this region's nucleotide sequence