Experiment: D-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c20470 and PGA1_c20480 are separated by 84 nucleotides PGA1_c20480 and PGA1_c20490 are separated by 133 nucleotides PGA1_c20490 and PGA1_c20500 are separated by 129 nucleotides PGA1_c20500 and PGA1_c20510 are separated by 246 nucleotides PGA1_c20510 and PGA1_c20520 are separated by 190 nucleotides
PGA1_c20470: PGA1_c20470 - hypothetical protein, at 2,122,720 to 2,123,355
_c20470
PGA1_c20480: PGA1_c20480 - tRNA-Val, at 2,123,440 to 2,123,514
_c20480
PGA1_c20490: PGA1_c20490 - transcriptional regulator, AsnC family, at 2,123,648 to 2,124,103
_c20490
PGA1_c20500: PGA1_c20500 - leucine dehydrogenase Ldh, at 2,124,233 to 2,125,288
_c20500
PGA1_c20510: PGA1_c20510 - hypothetical protein, at 2,125,535 to 2,125,807
_c20510
PGA1_c20520: PGA1_c20520 - acetyltransferase and methytransferase domain-containing protein, at 2,125,998 to 2,127,035
_c20520
Position (kb)
2124
2125
2126 Strain fitness (log2 ratio)
-1
0
1
2 at 2123.321 kb on - strand at 2123.329 kb on + strand at 2123.329 kb on + strand at 2123.350 kb on + strand at 2123.353 kb on - strand at 2123.434 kb on + strand at 2123.500 kb on - strand, within PGA1_c20480 at 2123.500 kb on - strand, within PGA1_c20480 at 2123.529 kb on + strand at 2123.547 kb on + strand at 2123.555 kb on - strand at 2123.555 kb on - strand at 2123.673 kb on - strand at 2123.689 kb on + strand at 2123.689 kb on + strand at 2123.689 kb on + strand at 2123.697 kb on - strand, within PGA1_c20490 at 2123.697 kb on - strand, within PGA1_c20490 at 2123.697 kb on - strand, within PGA1_c20490 at 2123.697 kb on - strand, within PGA1_c20490 at 2123.697 kb on - strand, within PGA1_c20490 at 2123.697 kb on - strand, within PGA1_c20490 at 2123.697 kb on - strand, within PGA1_c20490 at 2123.697 kb on - strand, within PGA1_c20490 at 2123.697 kb on - strand, within PGA1_c20490 at 2123.697 kb on - strand, within PGA1_c20490 at 2123.801 kb on + strand, within PGA1_c20490 at 2123.841 kb on - strand, within PGA1_c20490 at 2123.923 kb on + strand, within PGA1_c20490 at 2123.933 kb on + strand, within PGA1_c20490 at 2124.089 kb on - strand at 2124.251 kb on - strand at 2124.257 kb on - strand at 2124.300 kb on + strand at 2124.303 kb on - strand at 2124.433 kb on - strand, within PGA1_c20500 at 2124.510 kb on + strand, within PGA1_c20500 at 2124.518 kb on - strand, within PGA1_c20500 at 2124.549 kb on + strand, within PGA1_c20500 at 2124.549 kb on + strand, within PGA1_c20500 at 2124.598 kb on + strand, within PGA1_c20500 at 2124.643 kb on + strand, within PGA1_c20500 at 2124.825 kb on - strand, within PGA1_c20500 at 2124.825 kb on - strand, within PGA1_c20500 at 2124.830 kb on - strand, within PGA1_c20500 at 2124.867 kb on - strand, within PGA1_c20500 at 2124.880 kb on - strand, within PGA1_c20500 at 2124.975 kb on - strand, within PGA1_c20500 at 2124.985 kb on + strand, within PGA1_c20500 at 2124.993 kb on - strand, within PGA1_c20500 at 2125.116 kb on - strand, within PGA1_c20500 at 2125.167 kb on + strand, within PGA1_c20500 at 2125.176 kb on + strand, within PGA1_c20500 at 2125.176 kb on + strand, within PGA1_c20500 at 2125.212 kb on - strand at 2125.212 kb on - strand at 2125.261 kb on + strand at 2125.296 kb on - strand at 2125.301 kb on - strand at 2125.311 kb on + strand at 2125.327 kb on - strand at 2125.415 kb on + strand at 2125.415 kb on + strand at 2125.423 kb on - strand at 2125.423 kb on - strand at 2125.467 kb on + strand at 2125.470 kb on - strand at 2125.502 kb on + strand at 2125.510 kb on - strand at 2125.510 kb on - strand at 2125.510 kb on - strand at 2125.510 kb on - strand at 2125.533 kb on + strand at 2125.546 kb on - strand at 2125.546 kb on - strand at 2125.585 kb on + strand, within PGA1_c20510 at 2125.585 kb on + strand, within PGA1_c20510 at 2125.585 kb on + strand, within PGA1_c20510 at 2125.586 kb on + strand, within PGA1_c20510 at 2125.593 kb on - strand, within PGA1_c20510 at 2125.632 kb on + strand, within PGA1_c20510 at 2125.640 kb on - strand, within PGA1_c20510 at 2125.706 kb on - strand, within PGA1_c20510 at 2125.757 kb on + strand, within PGA1_c20510 at 2125.861 kb on + strand at 2125.861 kb on + strand at 2125.869 kb on - strand at 2125.869 kb on - strand at 2125.994 kb on - strand at 2126.039 kb on + strand at 2126.120 kb on + strand, within PGA1_c20520 at 2126.135 kb on + strand, within PGA1_c20520 at 2126.143 kb on - strand, within PGA1_c20520 at 2126.193 kb on + strand, within PGA1_c20520 at 2126.206 kb on - strand, within PGA1_c20520 at 2126.218 kb on + strand, within PGA1_c20520 at 2126.280 kb on - strand, within PGA1_c20520
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine (N) remove 2,123,321 - -0.3 2,123,329 + -0.8 2,123,329 + -1.5 2,123,350 + -0.2 2,123,353 - +0.9 2,123,434 + +1.2 2,123,500 - PGA1_c20480 0.80 -0.0 2,123,500 - PGA1_c20480 0.80 +0.0 2,123,529 + -0.0 2,123,547 + -1.1 2,123,555 - -0.4 2,123,555 - -0.3 2,123,673 - +0.6 2,123,689 + +0.6 2,123,689 + -0.9 2,123,689 + -0.9 2,123,697 - PGA1_c20490 0.11 -0.3 2,123,697 - PGA1_c20490 0.11 -0.3 2,123,697 - PGA1_c20490 0.11 +0.5 2,123,697 - PGA1_c20490 0.11 +0.2 2,123,697 - PGA1_c20490 0.11 -0.2 2,123,697 - PGA1_c20490 0.11 +0.5 2,123,697 - PGA1_c20490 0.11 +1.1 2,123,697 - PGA1_c20490 0.11 +0.3 2,123,697 - PGA1_c20490 0.11 +0.5 2,123,697 - PGA1_c20490 0.11 -0.5 2,123,801 + PGA1_c20490 0.34 -1.2 2,123,841 - PGA1_c20490 0.42 +0.9 2,123,923 + PGA1_c20490 0.60 +0.3 2,123,933 + PGA1_c20490 0.62 +0.2 2,124,089 - +0.3 2,124,251 - -1.7 2,124,257 - -1.1 2,124,300 + -0.2 2,124,303 - +0.1 2,124,433 - PGA1_c20500 0.19 +0.1 2,124,510 + PGA1_c20500 0.26 +1.6 2,124,518 - PGA1_c20500 0.27 -0.2 2,124,549 + PGA1_c20500 0.30 +0.1 2,124,549 + PGA1_c20500 0.30 -0.2 2,124,598 + PGA1_c20500 0.35 +1.0 2,124,643 + PGA1_c20500 0.39 +0.3 2,124,825 - PGA1_c20500 0.56 +1.8 2,124,825 - PGA1_c20500 0.56 -1.5 2,124,830 - PGA1_c20500 0.57 +1.1 2,124,867 - PGA1_c20500 0.60 +0.1 2,124,880 - PGA1_c20500 0.61 -0.7 2,124,975 - PGA1_c20500 0.70 +0.5 2,124,985 + PGA1_c20500 0.71 +1.1 2,124,993 - PGA1_c20500 0.72 +0.0 2,125,116 - PGA1_c20500 0.84 +0.6 2,125,167 + PGA1_c20500 0.88 -0.7 2,125,176 + PGA1_c20500 0.89 +0.1 2,125,176 + PGA1_c20500 0.89 -0.2 2,125,212 - +1.2 2,125,212 - +1.0 2,125,261 + -0.1 2,125,296 - +1.1 2,125,301 - +1.1 2,125,311 + -0.1 2,125,327 - +0.7 2,125,415 + -0.4 2,125,415 + -0.2 2,125,423 - +0.8 2,125,423 - +0.5 2,125,467 + -0.2 2,125,470 - +0.3 2,125,502 + +0.9 2,125,510 - -0.2 2,125,510 - -0.8 2,125,510 - +0.7 2,125,510 - -1.4 2,125,533 + -0.0 2,125,546 - +0.9 2,125,546 - -0.9 2,125,585 + PGA1_c20510 0.18 +0.0 2,125,585 + PGA1_c20510 0.18 -0.4 2,125,585 + PGA1_c20510 0.18 -0.9 2,125,586 + PGA1_c20510 0.19 -0.1 2,125,593 - PGA1_c20510 0.21 +0.3 2,125,632 + PGA1_c20510 0.36 +0.5 2,125,640 - PGA1_c20510 0.38 +0.3 2,125,706 - PGA1_c20510 0.63 -0.5 2,125,757 + PGA1_c20510 0.81 -0.3 2,125,861 + +0.3 2,125,861 + +0.9 2,125,869 - -0.4 2,125,869 - +0.3 2,125,994 - +0.1 2,126,039 + +0.8 2,126,120 + PGA1_c20520 0.12 -0.4 2,126,135 + PGA1_c20520 0.13 +1.7 2,126,143 - PGA1_c20520 0.14 +0.5 2,126,193 + PGA1_c20520 0.19 -0.1 2,126,206 - PGA1_c20520 0.20 +1.1 2,126,218 + PGA1_c20520 0.21 -0.3 2,126,280 - PGA1_c20520 0.27 -0.1
Or see this region's nucleotide sequence