Experiment: D-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c19640 and PGA1_c19650 are separated by 165 nucleotides PGA1_c19650 and PGA1_c19660 overlap by 1 nucleotides PGA1_c19660 and PGA1_c19670 are separated by 14 nucleotides
PGA1_c19640: PGA1_c19640 - transcriptional regulator, containing response regulator domain, at 2,039,629 to 2,040,243
_c19640
PGA1_c19650: PGA1_c19650 - Protein of unknown function (DUF2474)., at 2,040,409 to 2,040,576
_c19650
PGA1_c19660: PGA1_c19660 - cytochrome d ubiquinol oxidase subunit 2, at 2,040,576 to 2,041,580
_c19660
PGA1_c19670: PGA1_c19670 - cytochrome d ubiquinol oxidase subunit 1, at 2,041,595 to 2,043,019
_c19670
Position (kb)
2040
2041
2042 Strain fitness (log2 ratio)
-2
-1
0
1 at 2039.599 kb on + strand at 2039.605 kb on + strand at 2039.607 kb on - strand at 2039.613 kb on + strand at 2039.613 kb on - strand at 2039.707 kb on + strand, within PGA1_c19640 at 2039.707 kb on + strand, within PGA1_c19640 at 2039.757 kb on + strand, within PGA1_c19640 at 2039.846 kb on - strand, within PGA1_c19640 at 2039.886 kb on - strand, within PGA1_c19640 at 2039.937 kb on + strand, within PGA1_c19640 at 2039.981 kb on - strand, within PGA1_c19640 at 2040.031 kb on - strand, within PGA1_c19640 at 2040.056 kb on + strand, within PGA1_c19640 at 2040.064 kb on - strand, within PGA1_c19640 at 2040.093 kb on + strand, within PGA1_c19640 at 2040.136 kb on - strand, within PGA1_c19640 at 2040.237 kb on + strand at 2040.237 kb on + strand at 2040.284 kb on + strand at 2040.325 kb on - strand at 2040.358 kb on - strand at 2040.384 kb on + strand at 2040.391 kb on - strand at 2040.392 kb on - strand at 2040.392 kb on - strand at 2040.415 kb on - strand at 2040.443 kb on + strand, within PGA1_c19650 at 2040.448 kb on + strand, within PGA1_c19650 at 2040.448 kb on + strand, within PGA1_c19650 at 2040.448 kb on + strand, within PGA1_c19650 at 2040.448 kb on + strand, within PGA1_c19650 at 2040.448 kb on + strand, within PGA1_c19650 at 2040.448 kb on + strand, within PGA1_c19650 at 2040.456 kb on - strand, within PGA1_c19650 at 2040.456 kb on - strand, within PGA1_c19650 at 2040.456 kb on - strand, within PGA1_c19650 at 2040.456 kb on - strand, within PGA1_c19650 at 2040.456 kb on - strand, within PGA1_c19650 at 2040.456 kb on - strand, within PGA1_c19650 at 2040.456 kb on - strand, within PGA1_c19650 at 2040.456 kb on - strand, within PGA1_c19650 at 2040.478 kb on - strand, within PGA1_c19650 at 2040.521 kb on + strand, within PGA1_c19650 at 2040.598 kb on - strand at 2040.647 kb on - strand at 2040.720 kb on + strand, within PGA1_c19660 at 2040.843 kb on - strand, within PGA1_c19660 at 2040.850 kb on + strand, within PGA1_c19660 at 2040.858 kb on - strand, within PGA1_c19660 at 2040.886 kb on - strand, within PGA1_c19660 at 2040.951 kb on - strand, within PGA1_c19660 at 2040.957 kb on + strand, within PGA1_c19660 at 2040.975 kb on - strand, within PGA1_c19660 at 2041.165 kb on + strand, within PGA1_c19660 at 2041.165 kb on - strand, within PGA1_c19660 at 2041.173 kb on - strand, within PGA1_c19660 at 2041.192 kb on - strand, within PGA1_c19660 at 2041.324 kb on + strand, within PGA1_c19660 at 2041.332 kb on - strand, within PGA1_c19660 at 2041.347 kb on - strand, within PGA1_c19660 at 2041.351 kb on + strand, within PGA1_c19660 at 2041.519 kb on + strand at 2041.519 kb on + strand at 2041.519 kb on + strand at 2041.525 kb on - strand at 2041.527 kb on - strand at 2041.527 kb on - strand at 2041.532 kb on - strand at 2041.547 kb on + strand at 2041.575 kb on - strand at 2041.582 kb on + strand at 2041.585 kb on - strand at 2041.687 kb on + strand at 2041.826 kb on + strand, within PGA1_c19670 at 2041.833 kb on - strand, within PGA1_c19670 at 2041.843 kb on - strand, within PGA1_c19670 at 2041.886 kb on - strand, within PGA1_c19670 at 2041.952 kb on + strand, within PGA1_c19670 at 2042.012 kb on - strand, within PGA1_c19670 at 2042.076 kb on - strand, within PGA1_c19670 at 2042.111 kb on + strand, within PGA1_c19670 at 2042.128 kb on + strand, within PGA1_c19670 at 2042.192 kb on - strand, within PGA1_c19670 at 2042.194 kb on + strand, within PGA1_c19670 at 2042.272 kb on + strand, within PGA1_c19670 at 2042.289 kb on - strand, within PGA1_c19670 at 2042.349 kb on + strand, within PGA1_c19670 at 2042.420 kb on - strand, within PGA1_c19670 at 2042.425 kb on + strand, within PGA1_c19670 at 2042.511 kb on + strand, within PGA1_c19670
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine (N) remove 2,039,599 + -1.1 2,039,605 + +0.6 2,039,607 - -0.0 2,039,613 + +0.0 2,039,613 - +0.0 2,039,707 + PGA1_c19640 0.13 +0.0 2,039,707 + PGA1_c19640 0.13 -0.9 2,039,757 + PGA1_c19640 0.21 +0.2 2,039,846 - PGA1_c19640 0.35 +0.2 2,039,886 - PGA1_c19640 0.42 -0.2 2,039,937 + PGA1_c19640 0.50 -0.5 2,039,981 - PGA1_c19640 0.57 +0.3 2,040,031 - PGA1_c19640 0.65 -0.1 2,040,056 + PGA1_c19640 0.69 +0.1 2,040,064 - PGA1_c19640 0.71 +0.3 2,040,093 + PGA1_c19640 0.75 +0.6 2,040,136 - PGA1_c19640 0.82 -0.5 2,040,237 + +0.0 2,040,237 + -1.3 2,040,284 + -0.3 2,040,325 - -0.1 2,040,358 - +0.5 2,040,384 + -0.3 2,040,391 - +0.3 2,040,392 - +0.2 2,040,392 - +0.1 2,040,415 - +0.1 2,040,443 + PGA1_c19650 0.20 +1.3 2,040,448 + PGA1_c19650 0.23 +0.4 2,040,448 + PGA1_c19650 0.23 -1.6 2,040,448 + PGA1_c19650 0.23 -1.3 2,040,448 + PGA1_c19650 0.23 +0.2 2,040,448 + PGA1_c19650 0.23 +0.9 2,040,448 + PGA1_c19650 0.23 -1.0 2,040,456 - PGA1_c19650 0.28 -0.3 2,040,456 - PGA1_c19650 0.28 +0.1 2,040,456 - PGA1_c19650 0.28 +1.0 2,040,456 - PGA1_c19650 0.28 -0.1 2,040,456 - PGA1_c19650 0.28 +0.1 2,040,456 - PGA1_c19650 0.28 +0.3 2,040,456 - PGA1_c19650 0.28 +0.6 2,040,456 - PGA1_c19650 0.28 -1.3 2,040,478 - PGA1_c19650 0.41 +0.0 2,040,521 + PGA1_c19650 0.67 +1.0 2,040,598 - -0.4 2,040,647 - +0.3 2,040,720 + PGA1_c19660 0.14 +1.5 2,040,843 - PGA1_c19660 0.27 +0.7 2,040,850 + PGA1_c19660 0.27 -0.5 2,040,858 - PGA1_c19660 0.28 +0.5 2,040,886 - PGA1_c19660 0.31 -1.1 2,040,951 - PGA1_c19660 0.37 +0.7 2,040,957 + PGA1_c19660 0.38 +0.9 2,040,975 - PGA1_c19660 0.40 +0.3 2,041,165 + PGA1_c19660 0.59 +0.5 2,041,165 - PGA1_c19660 0.59 +0.3 2,041,173 - PGA1_c19660 0.59 +0.4 2,041,192 - PGA1_c19660 0.61 +0.4 2,041,324 + PGA1_c19660 0.74 -1.2 2,041,332 - PGA1_c19660 0.75 -0.2 2,041,347 - PGA1_c19660 0.77 +1.7 2,041,351 + PGA1_c19660 0.77 -0.9 2,041,519 + +0.3 2,041,519 + -0.7 2,041,519 + -0.1 2,041,525 - +0.9 2,041,527 - -1.0 2,041,527 - -0.1 2,041,532 - +0.4 2,041,547 + -0.2 2,041,575 - -0.3 2,041,582 + +0.4 2,041,585 - -0.7 2,041,687 + +0.9 2,041,826 + PGA1_c19670 0.16 +0.4 2,041,833 - PGA1_c19670 0.17 -0.5 2,041,843 - PGA1_c19670 0.17 +0.3 2,041,886 - PGA1_c19670 0.20 -0.1 2,041,952 + PGA1_c19670 0.25 +0.0 2,042,012 - PGA1_c19670 0.29 +1.1 2,042,076 - PGA1_c19670 0.34 +1.3 2,042,111 + PGA1_c19670 0.36 -0.6 2,042,128 + PGA1_c19670 0.37 -1.1 2,042,192 - PGA1_c19670 0.42 +0.0 2,042,194 + PGA1_c19670 0.42 +0.3 2,042,272 + PGA1_c19670 0.48 +0.5 2,042,289 - PGA1_c19670 0.49 -1.8 2,042,349 + PGA1_c19670 0.53 +1.1 2,042,420 - PGA1_c19670 0.58 +0.3 2,042,425 + PGA1_c19670 0.58 -0.6 2,042,511 + PGA1_c19670 0.64 +0.4
Or see this region's nucleotide sequence