Experiment: D-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c19170 and PGA1_c19180 are separated by 233 nucleotides PGA1_c19180 and PGA1_c19190 overlap by 4 nucleotides PGA1_c19190 and PGA1_c19200 are separated by 5 nucleotides PGA1_c19200 and PGA1_c19210 are separated by 162 nucleotides
PGA1_c19170: PGA1_c19170 - sarcosine oxidase subunit beta, at 1,990,583 to 1,991,827
_c19170
PGA1_c19180: PGA1_c19180 - cytochrome c-type biogenesis protein CycH, at 1,992,061 to 1,993,317
_c19180
PGA1_c19190: PGA1_c19190 - RNAse H-fold protein YqgF, at 1,993,314 to 1,993,787
_c19190
PGA1_c19200: PGA1_c19200 - Predicted Fe-S protein, at 1,993,793 to 1,994,026
_c19200
PGA1_c19210: PGA1_c19210 - hypothetical protein, at 1,994,189 to 1,994,983
_c19210
Position (kb)
1992
1993
1994 Strain fitness (log2 ratio)
-2
-1
0
1 at 1991.216 kb on + strand, within PGA1_c19170 at 1991.226 kb on - strand, within PGA1_c19170 at 1991.270 kb on - strand, within PGA1_c19170 at 1991.431 kb on + strand, within PGA1_c19170 at 1991.454 kb on - strand, within PGA1_c19170 at 1991.457 kb on + strand, within PGA1_c19170 at 1991.546 kb on + strand, within PGA1_c19170 at 1991.595 kb on - strand, within PGA1_c19170 at 1991.612 kb on - strand, within PGA1_c19170 at 1991.778 kb on + strand at 1991.860 kb on - strand at 1991.868 kb on - strand at 1991.876 kb on + strand at 1991.884 kb on - strand at 1991.922 kb on - strand at 1991.978 kb on + strand at 1992.031 kb on + strand at 1993.106 kb on + strand, within PGA1_c19180 at 1993.157 kb on + strand, within PGA1_c19180 at 1993.245 kb on + strand at 1993.780 kb on - strand at 1993.790 kb on - strand at 1993.870 kb on + strand, within PGA1_c19200 at 1993.948 kb on + strand, within PGA1_c19200 at 1993.956 kb on - strand, within PGA1_c19200 at 1993.956 kb on - strand, within PGA1_c19200 at 1993.956 kb on - strand, within PGA1_c19200 at 1994.054 kb on + strand at 1994.064 kb on + strand at 1994.186 kb on - strand at 1994.201 kb on + strand at 1994.202 kb on + strand at 1994.258 kb on + strand at 1994.269 kb on - strand, within PGA1_c19210 at 1994.301 kb on - strand, within PGA1_c19210 at 1994.308 kb on + strand, within PGA1_c19210 at 1994.316 kb on - strand, within PGA1_c19210
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine (N) remove 1,991,216 + PGA1_c19170 0.51 -0.3 1,991,226 - PGA1_c19170 0.52 -0.5 1,991,270 - PGA1_c19170 0.55 -1.7 1,991,431 + PGA1_c19170 0.68 +0.5 1,991,454 - PGA1_c19170 0.70 -0.2 1,991,457 + PGA1_c19170 0.70 +0.1 1,991,546 + PGA1_c19170 0.77 -0.3 1,991,595 - PGA1_c19170 0.81 +0.4 1,991,612 - PGA1_c19170 0.83 +0.4 1,991,778 + -0.2 1,991,860 - -0.2 1,991,868 - +0.4 1,991,876 + -0.4 1,991,884 - -0.1 1,991,922 - +0.1 1,991,978 + +0.3 1,992,031 + -0.3 1,993,106 + PGA1_c19180 0.83 -0.1 1,993,157 + PGA1_c19180 0.87 +1.3 1,993,245 + +0.7 1,993,780 - -0.9 1,993,790 - -1.1 1,993,870 + PGA1_c19200 0.33 -1.5 1,993,948 + PGA1_c19200 0.66 -0.5 1,993,956 - PGA1_c19200 0.70 -1.7 1,993,956 - PGA1_c19200 0.70 -0.2 1,993,956 - PGA1_c19200 0.70 -2.4 1,994,054 + -1.1 1,994,064 + -0.1 1,994,186 - -0.7 1,994,201 + +0.5 1,994,202 + +0.0 1,994,258 + -0.5 1,994,269 - PGA1_c19210 0.10 +0.4 1,994,301 - PGA1_c19210 0.14 +1.3 1,994,308 + PGA1_c19210 0.15 +0.3 1,994,316 - PGA1_c19210 0.16 -0.9
Or see this region's nucleotide sequence