Experiment: Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 1x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS25315 and BWI76_RS25320 are separated by 9 nucleotides BWI76_RS25320 and BWI76_RS25325 are separated by 79 nucleotides BWI76_RS25325 and BWI76_RS25330 are separated by 92 nucleotides
BWI76_RS25315: BWI76_RS25315 - glutamate synthase large subunit, at 5,261,068 to 5,265,528
_RS25315
BWI76_RS25320: BWI76_RS25320 - glutamate synthase, at 5,265,538 to 5,266,956
_RS25320
BWI76_RS25325: BWI76_RS25325 - hypothetical protein, at 5,267,036 to 5,267,443
_RS25325
BWI76_RS25330: BWI76_RS25330 - stringent starvation protein B, at 5,267,536 to 5,268,024
_RS25330
Position (kb)
5265
5266
5267 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 5264.659 kb on + strand, within BWI76_RS25315 at 5264.672 kb on - strand, within BWI76_RS25315 at 5264.763 kb on - strand, within BWI76_RS25315 at 5264.768 kb on + strand, within BWI76_RS25315 at 5264.919 kb on - strand, within BWI76_RS25315 at 5265.056 kb on - strand, within BWI76_RS25315 at 5265.502 kb on + strand at 5265.748 kb on - strand, within BWI76_RS25320 at 5265.967 kb on - strand, within BWI76_RS25320 at 5266.009 kb on + strand, within BWI76_RS25320 at 5266.228 kb on + strand, within BWI76_RS25320 at 5266.268 kb on - strand, within BWI76_RS25320 at 5266.308 kb on + strand, within BWI76_RS25320 at 5266.432 kb on + strand, within BWI76_RS25320 at 5266.432 kb on + strand, within BWI76_RS25320 at 5266.561 kb on - strand, within BWI76_RS25320 at 5266.611 kb on + strand, within BWI76_RS25320 at 5266.691 kb on + strand, within BWI76_RS25320 at 5267.041 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 1x remove 5,264,659 + BWI76_RS25315 0.80 -3.6 5,264,672 - BWI76_RS25315 0.81 -4.4 5,264,763 - BWI76_RS25315 0.83 -2.8 5,264,768 + BWI76_RS25315 0.83 -4.6 5,264,919 - BWI76_RS25315 0.86 -2.7 5,265,056 - BWI76_RS25315 0.89 +0.2 5,265,502 + -1.6 5,265,748 - BWI76_RS25320 0.15 -2.7 5,265,967 - BWI76_RS25320 0.30 -1.5 5,266,009 + BWI76_RS25320 0.33 -1.4 5,266,228 + BWI76_RS25320 0.49 -4.0 5,266,268 - BWI76_RS25320 0.51 -3.0 5,266,308 + BWI76_RS25320 0.54 -4.1 5,266,432 + BWI76_RS25320 0.63 -4.1 5,266,432 + BWI76_RS25320 0.63 -4.0 5,266,561 - BWI76_RS25320 0.72 -3.6 5,266,611 + BWI76_RS25320 0.76 -5.3 5,266,691 + BWI76_RS25320 0.81 -6.6 5,267,041 - -0.5
Or see this region's nucleotide sequence