Experiment: L-Tryptophan (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_65p00170 and PGA1_65p00180 are separated by 354 nucleotides PGA1_65p00180 and PGA1_65p00190 are separated by 21 nucleotides
PGA1_65p00170: PGA1_65p00170 - putative capsular polysaccharide biosynthesis protein, at 22,292 to 24,139
_65p00170
PGA1_65p00180: PGA1_65p00180 - putative sugar transferase, at 24,494 to 25,105
_65p00180
PGA1_65p00190: PGA1_65p00190 - putative NAD dependent epimerase/dehydratase, at 25,127 to 26,176
_65p00190
Position (kb) 24
25
26 Strain fitness (log2 ratio) -2
-1
0
1
2 at 23.504 kb on - strand, within PGA1_65p00170 at 23.514 kb on - strand, within PGA1_65p00170 at 23.723 kb on - strand, within PGA1_65p00170 at 23.833 kb on - strand, within PGA1_65p00170 at 23.838 kb on - strand, within PGA1_65p00170 at 23.856 kb on - strand, within PGA1_65p00170 at 23.879 kb on + strand, within PGA1_65p00170 at 23.894 kb on - strand, within PGA1_65p00170 at 23.926 kb on + strand, within PGA1_65p00170 at 24.023 kb on + strand at 24.023 kb on + strand at 24.046 kb on - strand at 24.052 kb on + strand at 24.092 kb on + strand at 24.184 kb on + strand at 24.294 kb on + strand at 24.302 kb on - strand at 24.394 kb on - strand at 24.428 kb on - strand at 24.490 kb on + strand at 24.498 kb on - strand at 24.612 kb on + strand, within PGA1_65p00180 at 24.926 kb on + strand, within PGA1_65p00180 at 24.944 kb on + strand, within PGA1_65p00180 at 24.963 kb on - strand, within PGA1_65p00180 at 24.971 kb on - strand, within PGA1_65p00180 at 24.971 kb on - strand, within PGA1_65p00180 at 24.989 kb on - strand, within PGA1_65p00180 at 25.057 kb on + strand at 25.144 kb on - strand at 25.257 kb on + strand, within PGA1_65p00190 at 25.392 kb on - strand, within PGA1_65p00190
Per-strain Table
Position Strand Gene LocusTag Fraction L-Tryptophan (N) remove 23,504 - PGA1_65p00170 0.66 +0.4 23,514 - PGA1_65p00170 0.66 +1.2 23,723 - PGA1_65p00170 0.77 -0.1 23,833 - PGA1_65p00170 0.83 +0.6 23,838 - PGA1_65p00170 0.84 +0.2 23,856 - PGA1_65p00170 0.85 -1.4 23,879 + PGA1_65p00170 0.86 +1.2 23,894 - PGA1_65p00170 0.87 +0.3 23,926 + PGA1_65p00170 0.88 +0.9 24,023 + -0.6 24,023 + -0.8 24,046 - -0.9 24,052 + +1.0 24,092 + -0.6 24,184 + +1.2 24,294 + +0.0 24,302 - +1.0 24,394 - -0.4 24,428 - -0.2 24,490 + +1.5 24,498 - +0.0 24,612 + PGA1_65p00180 0.19 +0.7 24,926 + PGA1_65p00180 0.71 -0.8 24,944 + PGA1_65p00180 0.74 +1.2 24,963 - PGA1_65p00180 0.77 -0.8 24,971 - PGA1_65p00180 0.78 +1.4 24,971 - PGA1_65p00180 0.78 +2.3 24,989 - PGA1_65p00180 0.81 +0.6 25,057 + -0.4 25,144 - -1.8 25,257 + PGA1_65p00190 0.12 -0.8 25,392 - PGA1_65p00190 0.25 +0.5
Or see this region's nucleotide sequence