Experiment: L-Tryptophan (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c32180 and PGA1_c32190 are separated by 94 nucleotides PGA1_c32190 and PGA1_c32200 overlap by 4 nucleotides PGA1_c32200 and PGA1_c32210 overlap by 4 nucleotides PGA1_c32210 and PGA1_c32220 overlap by 1 nucleotides
PGA1_c32180: PGA1_c32180 - hypothetical protein, at 3,374,776 to 3,375,765
_c32180
PGA1_c32190: PGA1_c32190 - 2-oxo-hepta-3-ene-1,7-dioic acid hydratase HpcG, at 3,375,860 to 3,376,660
_c32190
PGA1_c32200: PGA1_c32200 - fumarylacetoacetate hydrolase family protein, at 3,376,657 to 3,377,511
_c32200
PGA1_c32210: PGA1_c32210 - 3,4-dihydroxyphenylacetate 2,3-dioxygenase HpaD, at 3,377,508 to 3,378,491
_c32210
PGA1_c32220: PGA1_c32220 - 5-carboxymethyl-2-hydroxymuconic semialdehyde dehydrogenase HpcC, at 3,378,491 to 3,380,002
_c32220
Position (kb)
3376
3377
3378 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3375.684 kb on - strand at 3375.791 kb on + strand at 3375.820 kb on + strand at 3375.824 kb on - strand at 3375.870 kb on - strand at 3375.877 kb on + strand at 3375.885 kb on - strand at 3375.934 kb on - strand at 3376.024 kb on - strand, within PGA1_c32190 at 3376.072 kb on - strand, within PGA1_c32190 at 3376.101 kb on + strand, within PGA1_c32190 at 3376.101 kb on + strand, within PGA1_c32190 at 3376.101 kb on + strand, within PGA1_c32190 at 3376.104 kb on - strand, within PGA1_c32190 at 3376.109 kb on - strand, within PGA1_c32190 at 3376.109 kb on - strand, within PGA1_c32190 at 3376.109 kb on - strand, within PGA1_c32190 at 3376.111 kb on + strand, within PGA1_c32190 at 3376.111 kb on + strand, within PGA1_c32190 at 3376.125 kb on - strand, within PGA1_c32190 at 3376.184 kb on + strand, within PGA1_c32190 at 3376.200 kb on - strand, within PGA1_c32190 at 3376.217 kb on + strand, within PGA1_c32190 at 3376.224 kb on + strand, within PGA1_c32190 at 3376.225 kb on - strand, within PGA1_c32190 at 3376.228 kb on + strand, within PGA1_c32190 at 3376.233 kb on - strand, within PGA1_c32190 at 3376.294 kb on + strand, within PGA1_c32190 at 3376.303 kb on - strand, within PGA1_c32190 at 3376.400 kb on + strand, within PGA1_c32190 at 3376.427 kb on + strand, within PGA1_c32190 at 3376.470 kb on - strand, within PGA1_c32190 at 3376.470 kb on - strand, within PGA1_c32190 at 3376.470 kb on - strand, within PGA1_c32190 at 3376.512 kb on + strand, within PGA1_c32190 at 3376.512 kb on + strand, within PGA1_c32190 at 3376.520 kb on - strand, within PGA1_c32190 at 3376.520 kb on - strand, within PGA1_c32190 at 3376.520 kb on - strand, within PGA1_c32190 at 3376.533 kb on + strand, within PGA1_c32190 at 3376.538 kb on - strand, within PGA1_c32190 at 3376.541 kb on - strand, within PGA1_c32190 at 3376.541 kb on - strand, within PGA1_c32190 at 3376.554 kb on + strand, within PGA1_c32190 at 3376.562 kb on - strand, within PGA1_c32190 at 3376.610 kb on + strand at 3376.646 kb on + strand at 3376.646 kb on + strand at 3376.646 kb on + strand at 3376.647 kb on - strand at 3376.647 kb on - strand at 3376.647 kb on - strand at 3376.654 kb on - strand at 3376.654 kb on - strand at 3376.654 kb on - strand at 3376.720 kb on - strand at 3376.730 kb on + strand at 3376.758 kb on - strand, within PGA1_c32200 at 3376.768 kb on + strand, within PGA1_c32200 at 3376.803 kb on + strand, within PGA1_c32200 at 3376.803 kb on + strand, within PGA1_c32200 at 3376.803 kb on + strand, within PGA1_c32200 at 3376.803 kb on + strand, within PGA1_c32200 at 3376.803 kb on + strand, within PGA1_c32200 at 3376.803 kb on + strand, within PGA1_c32200 at 3376.811 kb on - strand, within PGA1_c32200 at 3376.819 kb on + strand, within PGA1_c32200 at 3376.907 kb on - strand, within PGA1_c32200 at 3376.912 kb on - strand, within PGA1_c32200 at 3377.034 kb on - strand, within PGA1_c32200 at 3377.053 kb on + strand, within PGA1_c32200 at 3377.070 kb on + strand, within PGA1_c32200 at 3377.078 kb on - strand, within PGA1_c32200 at 3377.098 kb on + strand, within PGA1_c32200 at 3377.160 kb on - strand, within PGA1_c32200 at 3377.258 kb on + strand, within PGA1_c32200 at 3377.311 kb on - strand, within PGA1_c32200 at 3377.334 kb on - strand, within PGA1_c32200 at 3377.334 kb on - strand, within PGA1_c32200 at 3377.334 kb on - strand, within PGA1_c32200 at 3377.361 kb on - strand, within PGA1_c32200 at 3377.361 kb on - strand, within PGA1_c32200 at 3377.380 kb on - strand, within PGA1_c32200 at 3377.384 kb on - strand, within PGA1_c32200 at 3377.400 kb on + strand, within PGA1_c32200 at 3377.466 kb on - strand at 3377.538 kb on - strand at 3377.589 kb on + strand at 3377.617 kb on + strand, within PGA1_c32210 at 3377.621 kb on + strand, within PGA1_c32210 at 3377.654 kb on + strand, within PGA1_c32210 at 3377.654 kb on + strand, within PGA1_c32210 at 3377.654 kb on + strand, within PGA1_c32210 at 3377.662 kb on - strand, within PGA1_c32210 at 3377.662 kb on - strand, within PGA1_c32210 at 3377.662 kb on - strand, within PGA1_c32210 at 3377.662 kb on - strand, within PGA1_c32210 at 3377.676 kb on + strand, within PGA1_c32210 at 3377.833 kb on + strand, within PGA1_c32210 at 3377.842 kb on - strand, within PGA1_c32210 at 3377.859 kb on + strand, within PGA1_c32210 at 3377.879 kb on + strand, within PGA1_c32210 at 3377.883 kb on + strand, within PGA1_c32210 at 3377.954 kb on - strand, within PGA1_c32210 at 3378.052 kb on + strand, within PGA1_c32210 at 3378.092 kb on + strand, within PGA1_c32210 at 3378.092 kb on + strand, within PGA1_c32210 at 3378.092 kb on + strand, within PGA1_c32210 at 3378.100 kb on - strand, within PGA1_c32210 at 3378.100 kb on - strand, within PGA1_c32210 at 3378.148 kb on - strand, within PGA1_c32210 at 3378.170 kb on - strand, within PGA1_c32210 at 3378.170 kb on - strand, within PGA1_c32210 at 3378.170 kb on - strand, within PGA1_c32210 at 3378.174 kb on + strand, within PGA1_c32210 at 3378.182 kb on - strand, within PGA1_c32210 at 3378.228 kb on + strand, within PGA1_c32210 at 3378.228 kb on + strand, within PGA1_c32210 at 3378.228 kb on + strand, within PGA1_c32210 at 3378.236 kb on - strand, within PGA1_c32210 at 3378.236 kb on - strand, within PGA1_c32210 at 3378.258 kb on - strand, within PGA1_c32210 at 3378.260 kb on - strand, within PGA1_c32210 at 3378.264 kb on + strand, within PGA1_c32210 at 3378.272 kb on - strand, within PGA1_c32210 at 3378.274 kb on - strand, within PGA1_c32210 at 3378.274 kb on - strand, within PGA1_c32210 at 3378.274 kb on - strand, within PGA1_c32210 at 3378.274 kb on - strand, within PGA1_c32210 at 3378.307 kb on - strand, within PGA1_c32210 at 3378.317 kb on - strand, within PGA1_c32210 at 3378.323 kb on + strand, within PGA1_c32210 at 3378.369 kb on - strand, within PGA1_c32210 at 3378.416 kb on + strand at 3378.458 kb on + strand at 3378.496 kb on - strand at 3378.498 kb on + strand at 3378.506 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Tryptophan (N) remove 3,375,684 - +0.7 3,375,791 + -0.1 3,375,820 + +0.5 3,375,824 - -0.9 3,375,870 - -0.4 3,375,877 + -0.9 3,375,885 - -0.3 3,375,934 - +0.2 3,376,024 - PGA1_c32190 0.20 -1.3 3,376,072 - PGA1_c32190 0.26 -1.1 3,376,101 + PGA1_c32190 0.30 +0.5 3,376,101 + PGA1_c32190 0.30 +0.7 3,376,101 + PGA1_c32190 0.30 +1.5 3,376,104 - PGA1_c32190 0.30 +0.4 3,376,109 - PGA1_c32190 0.31 +0.3 3,376,109 - PGA1_c32190 0.31 +1.7 3,376,109 - PGA1_c32190 0.31 -0.3 3,376,111 + PGA1_c32190 0.31 +2.0 3,376,111 + PGA1_c32190 0.31 +0.0 3,376,125 - PGA1_c32190 0.33 -1.2 3,376,184 + PGA1_c32190 0.40 +1.1 3,376,200 - PGA1_c32190 0.42 -1.8 3,376,217 + PGA1_c32190 0.45 +0.7 3,376,224 + PGA1_c32190 0.45 -0.8 3,376,225 - PGA1_c32190 0.46 +0.3 3,376,228 + PGA1_c32190 0.46 -1.1 3,376,233 - PGA1_c32190 0.47 +0.0 3,376,294 + PGA1_c32190 0.54 +0.6 3,376,303 - PGA1_c32190 0.55 -0.1 3,376,400 + PGA1_c32190 0.67 -0.7 3,376,427 + PGA1_c32190 0.71 +0.0 3,376,470 - PGA1_c32190 0.76 +1.0 3,376,470 - PGA1_c32190 0.76 +0.3 3,376,470 - PGA1_c32190 0.76 +0.8 3,376,512 + PGA1_c32190 0.81 +0.4 3,376,512 + PGA1_c32190 0.81 +0.6 3,376,520 - PGA1_c32190 0.82 +0.4 3,376,520 - PGA1_c32190 0.82 +0.0 3,376,520 - PGA1_c32190 0.82 -0.9 3,376,533 + PGA1_c32190 0.84 +0.2 3,376,538 - PGA1_c32190 0.85 -1.1 3,376,541 - PGA1_c32190 0.85 +1.1 3,376,541 - PGA1_c32190 0.85 +0.3 3,376,554 + PGA1_c32190 0.87 +1.6 3,376,562 - PGA1_c32190 0.88 +0.9 3,376,610 + +0.5 3,376,646 + +1.2 3,376,646 + -2.3 3,376,646 + +0.9 3,376,647 - -0.5 3,376,647 - -0.8 3,376,647 - -0.2 3,376,654 - +0.8 3,376,654 - +0.1 3,376,654 - -0.5 3,376,720 - +0.3 3,376,730 + -0.9 3,376,758 - PGA1_c32200 0.12 +2.1 3,376,768 + PGA1_c32200 0.13 +0.4 3,376,803 + PGA1_c32200 0.17 +0.1 3,376,803 + PGA1_c32200 0.17 +0.7 3,376,803 + PGA1_c32200 0.17 +0.7 3,376,803 + PGA1_c32200 0.17 -0.5 3,376,803 + PGA1_c32200 0.17 -2.2 3,376,803 + PGA1_c32200 0.17 +0.4 3,376,811 - PGA1_c32200 0.18 -0.5 3,376,819 + PGA1_c32200 0.19 +0.1 3,376,907 - PGA1_c32200 0.29 -0.3 3,376,912 - PGA1_c32200 0.30 -1.5 3,377,034 - PGA1_c32200 0.44 -0.7 3,377,053 + PGA1_c32200 0.46 -1.9 3,377,070 + PGA1_c32200 0.48 -1.0 3,377,078 - PGA1_c32200 0.49 -0.9 3,377,098 + PGA1_c32200 0.52 +0.3 3,377,160 - PGA1_c32200 0.59 -1.2 3,377,258 + PGA1_c32200 0.70 -0.7 3,377,311 - PGA1_c32200 0.76 +0.9 3,377,334 - PGA1_c32200 0.79 -3.0 3,377,334 - PGA1_c32200 0.79 -0.5 3,377,334 - PGA1_c32200 0.79 -1.3 3,377,361 - PGA1_c32200 0.82 +0.7 3,377,361 - PGA1_c32200 0.82 -0.4 3,377,380 - PGA1_c32200 0.85 +0.4 3,377,384 - PGA1_c32200 0.85 -1.2 3,377,400 + PGA1_c32200 0.87 -2.1 3,377,466 - -0.2 3,377,538 - -0.6 3,377,589 + -0.3 3,377,617 + PGA1_c32210 0.11 -0.7 3,377,621 + PGA1_c32210 0.11 +0.4 3,377,654 + PGA1_c32210 0.15 +0.3 3,377,654 + PGA1_c32210 0.15 -0.3 3,377,654 + PGA1_c32210 0.15 +0.9 3,377,662 - PGA1_c32210 0.16 -0.5 3,377,662 - PGA1_c32210 0.16 +0.3 3,377,662 - PGA1_c32210 0.16 -0.7 3,377,662 - PGA1_c32210 0.16 +0.0 3,377,676 + PGA1_c32210 0.17 -0.1 3,377,833 + PGA1_c32210 0.33 +0.8 3,377,842 - PGA1_c32210 0.34 -0.2 3,377,859 + PGA1_c32210 0.36 +1.2 3,377,879 + PGA1_c32210 0.38 -0.2 3,377,883 + PGA1_c32210 0.38 -0.0 3,377,954 - PGA1_c32210 0.45 -0.5 3,378,052 + PGA1_c32210 0.55 +0.5 3,378,092 + PGA1_c32210 0.59 -0.1 3,378,092 + PGA1_c32210 0.59 +0.7 3,378,092 + PGA1_c32210 0.59 +0.1 3,378,100 - PGA1_c32210 0.60 -0.2 3,378,100 - PGA1_c32210 0.60 +0.6 3,378,148 - PGA1_c32210 0.65 -0.7 3,378,170 - PGA1_c32210 0.67 +0.1 3,378,170 - PGA1_c32210 0.67 +0.5 3,378,170 - PGA1_c32210 0.67 +0.4 3,378,174 + PGA1_c32210 0.68 +0.0 3,378,182 - PGA1_c32210 0.68 -0.3 3,378,228 + PGA1_c32210 0.73 +0.0 3,378,228 + PGA1_c32210 0.73 +0.1 3,378,228 + PGA1_c32210 0.73 +0.5 3,378,236 - PGA1_c32210 0.74 +0.5 3,378,236 - PGA1_c32210 0.74 -1.9 3,378,258 - PGA1_c32210 0.76 -0.1 3,378,260 - PGA1_c32210 0.76 +0.3 3,378,264 + PGA1_c32210 0.77 +0.0 3,378,272 - PGA1_c32210 0.78 -1.3 3,378,274 - PGA1_c32210 0.78 -0.3 3,378,274 - PGA1_c32210 0.78 +0.6 3,378,274 - PGA1_c32210 0.78 +0.7 3,378,274 - PGA1_c32210 0.78 +0.3 3,378,307 - PGA1_c32210 0.81 -0.4 3,378,317 - PGA1_c32210 0.82 +0.2 3,378,323 + PGA1_c32210 0.83 +0.3 3,378,369 - PGA1_c32210 0.88 +0.3 3,378,416 + -0.2 3,378,458 + +0.5 3,378,496 - +0.9 3,378,498 + -1.4 3,378,506 - +0.8
Or see this region's nucleotide sequence