Experiment: PYE with CHIR-090 1 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_01994 and CCNA_01995 overlap by 13 nucleotides CCNA_01995 and CCNA_01996 overlap by 41 nucleotides CCNA_01996 and CCNA_01997 are separated by 51 nucleotides CCNA_01997 and CCNA_01998 are separated by 26 nucleotides
CCNA_01994: CCNA_01994 - 1-deoxy-D-xylulose 5-phosphate reductoisomerase, at 2,140,916 to 2,142,115
_01994
CCNA_01995: CCNA_01995 - phosphatidate cytidylyltransferase, at 2,142,103 to 2,142,948
_01995
CCNA_01996: CCNA_01996 - undecaprenyl pyrophosphate synthetase, at 2,142,908 to 2,143,687
_01996
CCNA_01997: CCNA_01997 - ribosome recycling factor (RRF), at 2,143,739 to 2,144,305
_01997
CCNA_01998: CCNA_01998 - uridylate kinase, at 2,144,332 to 2,145,072
_01998
Position (kb)
2142
2143
2144 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2142.113 kb on + strand at 2142.337 kb on - strand, within CCNA_01995 at 2142.337 kb on - strand, within CCNA_01995 at 2142.904 kb on - strand at 2143.710 kb on - strand at 2143.737 kb on + strand at 2144.324 kb on - strand at 2144.362 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 1 ug/mL remove 2,142,113 + +2.8 2,142,337 - CCNA_01995 0.28 -2.2 2,142,337 - CCNA_01995 0.28 -1.4 2,142,904 - +0.4 2,143,710 - +0.6 2,143,737 + -0.7 2,144,324 - +0.7 2,144,362 - +0.2
Or see this region's nucleotide sequence