Experiment: MoLS4 with Spermidine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nifK and nifD are separated by 36 nucleotides nifD and glnB-2 are separated by 11 nucleotides glnB-2 and glnB-3 are separated by 9 nucleotides
DVUA0011: nifK - nitrogenase molybdenum-iron protein beta chain (TIGR), at 14,801 to 16,186
nifK
DVUA0012: nifD - nitrogenase molybdenum-iron protein alpha chain (TIGR), at 16,223 to 17,851
nifD
DVUA0013: glnB-2 - nitrogen regulatory protein P-II (TIGR), at 17,863 to 18,258
glnB-2
DVUA0014: glnB-3 - nitrogen regulatory protein P-II (TIGR), at 18,268 to 18,594
glnB-3
Position (kb) 16
17
18 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 15.249 kb on + strand, within nifK at 15.336 kb on - strand, within nifK at 15.338 kb on + strand, within nifK at 15.348 kb on - strand, within nifK at 15.450 kb on - strand, within nifK at 15.533 kb on - strand, within nifK at 15.553 kb on - strand, within nifK at 15.565 kb on + strand, within nifK at 15.583 kb on + strand, within nifK at 15.593 kb on + strand, within nifK at 15.601 kb on - strand, within nifK at 15.634 kb on + strand, within nifK at 15.691 kb on - strand, within nifK at 15.702 kb on + strand, within nifK at 15.749 kb on + strand, within nifK at 15.789 kb on + strand, within nifK at 16.041 kb on + strand, within nifK at 16.046 kb on + strand, within nifK at 16.152 kb on - strand at 16.163 kb on + strand at 16.210 kb on + strand at 16.216 kb on - strand at 16.265 kb on + strand at 16.281 kb on - strand at 16.345 kb on - strand at 16.354 kb on - strand at 16.631 kb on - strand, within nifD at 16.632 kb on - strand, within nifD at 16.677 kb on + strand, within nifD at 16.695 kb on - strand, within nifD at 16.732 kb on + strand, within nifD at 16.761 kb on + strand, within nifD at 16.856 kb on - strand, within nifD at 16.856 kb on - strand, within nifD at 16.856 kb on - strand, within nifD at 16.866 kb on - strand, within nifD at 16.920 kb on + strand, within nifD at 16.923 kb on - strand, within nifD at 16.928 kb on - strand, within nifD at 16.994 kb on - strand, within nifD at 17.040 kb on + strand, within nifD at 17.064 kb on + strand, within nifD at 17.072 kb on - strand, within nifD at 17.100 kb on + strand, within nifD at 17.131 kb on - strand, within nifD at 17.173 kb on - strand, within nifD at 17.250 kb on + strand, within nifD at 17.282 kb on + strand, within nifD at 17.288 kb on - strand, within nifD at 17.295 kb on + strand, within nifD at 17.305 kb on - strand, within nifD at 17.366 kb on + strand, within nifD at 17.405 kb on - strand, within nifD at 17.438 kb on + strand, within nifD at 17.557 kb on + strand, within nifD at 17.600 kb on + strand, within nifD at 17.655 kb on - strand, within nifD at 17.668 kb on + strand, within nifD at 17.668 kb on + strand, within nifD at 17.694 kb on + strand at 17.701 kb on - strand at 17.786 kb on + strand at 17.831 kb on - strand at 17.843 kb on - strand at 17.919 kb on - strand, within glnB-2 at 17.997 kb on + strand, within glnB-2 at 18.002 kb on + strand, within glnB-2 at 18.061 kb on - strand, within glnB-2 at 18.085 kb on - strand, within glnB-2 at 18.186 kb on + strand, within glnB-2 at 18.242 kb on - strand at 18.282 kb on + strand at 18.296 kb on + strand at 18.296 kb on + strand at 18.296 kb on + strand at 18.304 kb on - strand, within glnB-3 at 18.304 kb on - strand, within glnB-3 at 18.304 kb on - strand, within glnB-3 at 18.304 kb on - strand, within glnB-3 at 18.353 kb on + strand, within glnB-3 at 18.370 kb on + strand, within glnB-3 at 18.378 kb on - strand, within glnB-3 at 18.404 kb on + strand, within glnB-3 at 18.404 kb on + strand, within glnB-3 at 18.431 kb on + strand, within glnB-3 at 18.439 kb on - strand, within glnB-3 at 18.439 kb on - strand, within glnB-3 at 18.466 kb on - strand, within glnB-3 at 18.473 kb on - strand, within glnB-3 at 18.492 kb on - strand, within glnB-3 at 18.506 kb on - strand, within glnB-3 at 18.527 kb on + strand, within glnB-3 at 18.535 kb on - strand, within glnB-3 at 18.559 kb on - strand, within glnB-3 at 18.747 kb on + strand at 18.755 kb on - strand at 18.849 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with Spermidine (N) remove 15,249 + nifK DVUA0011 0.32 -4.6 15,336 - nifK DVUA0011 0.39 -2.0 15,338 + nifK DVUA0011 0.39 -4.6 15,348 - nifK DVUA0011 0.39 -5.4 15,450 - nifK DVUA0011 0.47 -4.8 15,533 - nifK DVUA0011 0.53 -2.4 15,553 - nifK DVUA0011 0.54 -3.2 15,565 + nifK DVUA0011 0.55 -4.3 15,583 + nifK DVUA0011 0.56 -2.6 15,593 + nifK DVUA0011 0.57 -4.2 15,601 - nifK DVUA0011 0.58 -4.3 15,634 + nifK DVUA0011 0.60 -4.0 15,691 - nifK DVUA0011 0.64 -3.8 15,702 + nifK DVUA0011 0.65 -4.8 15,749 + nifK DVUA0011 0.68 -4.7 15,789 + nifK DVUA0011 0.71 -4.8 16,041 + nifK DVUA0011 0.89 -1.5 16,046 + nifK DVUA0011 0.90 -3.3 16,152 - -4.0 16,163 + -2.3 16,210 + -3.3 16,216 - -3.3 16,265 + -2.1 16,281 - -5.0 16,345 - -4.2 16,354 - -2.9 16,631 - nifD DVUA0012 0.25 -3.7 16,632 - nifD DVUA0012 0.25 -3.6 16,677 + nifD DVUA0012 0.28 -3.6 16,695 - nifD DVUA0012 0.29 -3.2 16,732 + nifD DVUA0012 0.31 -4.7 16,761 + nifD DVUA0012 0.33 -2.1 16,856 - nifD DVUA0012 0.39 -3.7 16,856 - nifD DVUA0012 0.39 -5.3 16,856 - nifD DVUA0012 0.39 -3.1 16,866 - nifD DVUA0012 0.39 -4.3 16,920 + nifD DVUA0012 0.43 -1.9 16,923 - nifD DVUA0012 0.43 -4.1 16,928 - nifD DVUA0012 0.43 -4.1 16,994 - nifD DVUA0012 0.47 -4.4 17,040 + nifD DVUA0012 0.50 -1.5 17,064 + nifD DVUA0012 0.52 -3.8 17,072 - nifD DVUA0012 0.52 -3.1 17,100 + nifD DVUA0012 0.54 -3.6 17,131 - nifD DVUA0012 0.56 -2.5 17,173 - nifD DVUA0012 0.58 -3.6 17,250 + nifD DVUA0012 0.63 -4.7 17,282 + nifD DVUA0012 0.65 -4.7 17,288 - nifD DVUA0012 0.65 -0.7 17,295 + nifD DVUA0012 0.66 -4.2 17,305 - nifD DVUA0012 0.66 -4.0 17,366 + nifD DVUA0012 0.70 -3.5 17,405 - nifD DVUA0012 0.73 -4.4 17,438 + nifD DVUA0012 0.75 -4.8 17,557 + nifD DVUA0012 0.82 -3.9 17,600 + nifD DVUA0012 0.85 -3.1 17,655 - nifD DVUA0012 0.88 -4.4 17,668 + nifD DVUA0012 0.89 -1.6 17,668 + nifD DVUA0012 0.89 -3.9 17,694 + -4.8 17,701 - -4.6 17,786 + -4.2 17,831 - -4.2 17,843 - -2.1 17,919 - glnB-2 DVUA0013 0.14 -2.5 17,997 + glnB-2 DVUA0013 0.34 -3.4 18,002 + glnB-2 DVUA0013 0.35 -3.7 18,061 - glnB-2 DVUA0013 0.50 -2.5 18,085 - glnB-2 DVUA0013 0.56 -2.8 18,186 + glnB-2 DVUA0013 0.82 -3.2 18,242 - -3.1 18,282 + -2.8 18,296 + -1.3 18,296 + -2.3 18,296 + -1.5 18,304 - glnB-3 DVUA0014 0.11 -2.4 18,304 - glnB-3 DVUA0014 0.11 -2.3 18,304 - glnB-3 DVUA0014 0.11 -1.8 18,304 - glnB-3 DVUA0014 0.11 -3.0 18,353 + glnB-3 DVUA0014 0.26 -3.1 18,370 + glnB-3 DVUA0014 0.31 -3.2 18,378 - glnB-3 DVUA0014 0.34 -0.7 18,404 + glnB-3 DVUA0014 0.42 -3.1 18,404 + glnB-3 DVUA0014 0.42 -3.0 18,431 + glnB-3 DVUA0014 0.50 -1.8 18,439 - glnB-3 DVUA0014 0.52 -2.2 18,439 - glnB-3 DVUA0014 0.52 -3.1 18,466 - glnB-3 DVUA0014 0.61 -2.6 18,473 - glnB-3 DVUA0014 0.63 -3.5 18,492 - glnB-3 DVUA0014 0.69 -2.8 18,506 - glnB-3 DVUA0014 0.73 -2.5 18,527 + glnB-3 DVUA0014 0.79 -3.0 18,535 - glnB-3 DVUA0014 0.82 -2.8 18,559 - glnB-3 DVUA0014 0.89 -5.1 18,747 + -2.9 18,755 - -2.3 18,849 - -0.7
Or see this region's nucleotide sequence