Experiment: MoLS4 with Spermidine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt wbaZ-2 and DVU0281 are separated by 104 nucleotides DVU0281 and mutY are separated by 239 nucleotides
DVU0280: wbaZ-2 - glycosyl transferase, group 1 family protein (TIGR), at 324,397 to 325,536
wbaZ-2
DVU0281: DVU0281 - exopolysaccharide biosynthesis protein, putative (TIGR), at 325,641 to 327,074
DVU0281
DVU0282: mutY - A/G-specific adenine glycosylase (TIGR), at 327,314 to 328,435
mutY
Position (kb) 325
326
327
328 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 324.712 kb on + strand, within wbaZ-2 at 324.712 kb on + strand, within wbaZ-2 at 324.824 kb on - strand, within wbaZ-2 at 324.868 kb on + strand, within wbaZ-2 at 324.982 kb on + strand, within wbaZ-2 at 325.131 kb on - strand, within wbaZ-2 at 325.189 kb on + strand, within wbaZ-2 at 325.196 kb on + strand, within wbaZ-2 at 325.221 kb on - strand, within wbaZ-2 at 325.221 kb on - strand, within wbaZ-2 at 325.223 kb on + strand, within wbaZ-2 at 325.235 kb on + strand, within wbaZ-2 at 325.310 kb on - strand, within wbaZ-2 at 325.310 kb on - strand, within wbaZ-2 at 325.567 kb on + strand at 325.896 kb on + strand, within DVU0281 at 326.087 kb on + strand, within DVU0281 at 326.104 kb on - strand, within DVU0281 at 326.242 kb on - strand, within DVU0281 at 326.284 kb on - strand, within DVU0281 at 326.783 kb on + strand, within DVU0281 at 326.796 kb on - strand, within DVU0281 at 326.895 kb on + strand, within DVU0281 at 327.011 kb on + strand at 327.130 kb on - strand at 327.312 kb on + strand at 327.319 kb on + strand at 327.319 kb on + strand at 327.320 kb on - strand at 327.327 kb on - strand at 327.337 kb on - strand at 327.337 kb on - strand at 327.379 kb on + strand at 327.387 kb on - strand at 327.445 kb on + strand, within mutY at 327.453 kb on - strand, within mutY at 327.487 kb on - strand, within mutY at 327.494 kb on + strand, within mutY at 327.499 kb on + strand, within mutY at 327.547 kb on - strand, within mutY at 327.669 kb on - strand, within mutY at 327.722 kb on - strand, within mutY at 327.743 kb on - strand, within mutY at 327.752 kb on + strand, within mutY at 327.757 kb on + strand, within mutY at 327.760 kb on - strand, within mutY at 327.877 kb on - strand, within mutY at 327.908 kb on - strand, within mutY at 327.960 kb on - strand, within mutY at 327.976 kb on + strand, within mutY at 327.978 kb on + strand, within mutY at 328.025 kb on + strand, within mutY at 328.071 kb on - strand, within mutY
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with Spermidine (N) remove 324,712 + wbaZ-2 DVU0280 0.28 -2.8 324,712 + wbaZ-2 DVU0280 0.28 -2.6 324,824 - wbaZ-2 DVU0280 0.37 -3.1 324,868 + wbaZ-2 DVU0280 0.41 -1.2 324,982 + wbaZ-2 DVU0280 0.51 -0.6 325,131 - wbaZ-2 DVU0280 0.64 -3.4 325,189 + wbaZ-2 DVU0280 0.69 -1.4 325,196 + wbaZ-2 DVU0280 0.70 +0.0 325,221 - wbaZ-2 DVU0280 0.72 -2.7 325,221 - wbaZ-2 DVU0280 0.72 -1.8 325,223 + wbaZ-2 DVU0280 0.72 -2.0 325,235 + wbaZ-2 DVU0280 0.74 -2.0 325,310 - wbaZ-2 DVU0280 0.80 -0.4 325,310 - wbaZ-2 DVU0280 0.80 -1.9 325,567 + +0.7 325,896 + DVU0281 0.18 -2.5 326,087 + DVU0281 0.31 -3.5 326,104 - DVU0281 0.32 -2.2 326,242 - DVU0281 0.42 -1.1 326,284 - DVU0281 0.45 -2.6 326,783 + DVU0281 0.80 -2.7 326,796 - DVU0281 0.81 -1.2 326,895 + DVU0281 0.87 -0.7 327,011 + -3.0 327,130 - +0.6 327,312 + -0.7 327,319 + +0.3 327,319 + -0.2 327,320 - +0.8 327,327 - +0.4 327,337 - -0.2 327,337 - -0.7 327,379 + +0.1 327,387 - -0.7 327,445 + mutY DVU0282 0.12 +0.1 327,453 - mutY DVU0282 0.12 -0.2 327,487 - mutY DVU0282 0.15 +0.4 327,494 + mutY DVU0282 0.16 -0.3 327,499 + mutY DVU0282 0.16 -0.2 327,547 - mutY DVU0282 0.21 +0.0 327,669 - mutY DVU0282 0.32 +0.4 327,722 - mutY DVU0282 0.36 +0.2 327,743 - mutY DVU0282 0.38 +0.2 327,752 + mutY DVU0282 0.39 -0.0 327,757 + mutY DVU0282 0.39 -0.5 327,760 - mutY DVU0282 0.40 -0.0 327,877 - mutY DVU0282 0.50 +0.4 327,908 - mutY DVU0282 0.53 -0.7 327,960 - mutY DVU0282 0.58 +0.3 327,976 + mutY DVU0282 0.59 +0.7 327,978 + mutY DVU0282 0.59 +0.3 328,025 + mutY DVU0282 0.63 +0.5 328,071 - mutY DVU0282 0.67 +0.6
Or see this region's nucleotide sequence