Experiment: MoLS4 with Spermidine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hisI and hisG overlap by 4 nucleotides hisG and aroE are separated by 99 nucleotides aroE and DVU0116 are separated by 338 nucleotides
DVU0113: hisI - phosphoribosyl-AMP cyclohydrolase (TIGR), at 147,117 to 147,497
hisI
DVU0114: hisG - ATP phosphoribosyltransferase (EC 2.4.2.17) (from data) , at 147,494 to 148,375
hisG
DVU0115: aroE - shikimate 5-dehydrogenase (TIGR), at 148,475 to 149,380
aroE
DVU0116: DVU0116 - polysaccharide deacetylase family protein (TIGR), at 149,719 to 150,795
DVU0116
Position (kb)
148
149
150 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 147.483 kb on - strand at 147.517 kb on - strand at 147.527 kb on + strand at 147.535 kb on - strand at 147.560 kb on - strand at 147.649 kb on - strand, within hisG at 147.680 kb on - strand, within hisG at 147.760 kb on + strand, within hisG at 147.821 kb on + strand, within hisG at 147.821 kb on + strand, within hisG at 147.828 kb on - strand, within hisG at 147.829 kb on - strand, within hisG at 147.829 kb on - strand, within hisG at 147.877 kb on - strand, within hisG at 147.894 kb on - strand, within hisG at 147.897 kb on - strand, within hisG at 147.946 kb on + strand, within hisG at 148.020 kb on - strand, within hisG at 148.071 kb on + strand, within hisG at 148.124 kb on - strand, within hisG at 148.172 kb on - strand, within hisG at 148.221 kb on + strand, within hisG at 148.280 kb on + strand, within hisG at 148.311 kb on + strand at 148.327 kb on + strand at 148.341 kb on - strand at 148.355 kb on + strand at 148.355 kb on + strand at 148.385 kb on + strand at 148.471 kb on + strand at 148.495 kb on - strand at 148.577 kb on + strand, within aroE at 149.321 kb on + strand at 149.321 kb on + strand at 149.321 kb on + strand at 149.322 kb on + strand at 149.324 kb on - strand at 149.329 kb on - strand at 149.374 kb on - strand at 149.375 kb on - strand at 149.389 kb on + strand at 149.436 kb on + strand at 149.436 kb on + strand at 149.499 kb on - strand at 149.535 kb on + strand at 149.563 kb on + strand at 149.571 kb on - strand at 149.595 kb on + strand at 149.608 kb on + strand at 149.637 kb on + strand at 149.676 kb on + strand at 149.821 kb on + strand at 149.892 kb on - strand, within DVU0116 at 149.901 kb on + strand, within DVU0116 at 149.918 kb on + strand, within DVU0116 at 149.966 kb on - strand, within DVU0116 at 150.035 kb on + strand, within DVU0116 at 150.195 kb on + strand, within DVU0116 at 150.195 kb on + strand, within DVU0116 at 150.195 kb on + strand, within DVU0116 at 150.195 kb on + strand, within DVU0116 at 150.196 kb on + strand, within DVU0116 at 150.197 kb on + strand, within DVU0116 at 150.203 kb on - strand, within DVU0116 at 150.217 kb on - strand, within DVU0116 at 150.219 kb on - strand, within DVU0116 at 150.248 kb on + strand, within DVU0116
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with Spermidine (N) remove 147,483 - -4.6 147,517 - -1.4 147,527 + -3.7 147,535 - -2.7 147,560 - -3.2 147,649 - hisG DVU0114 0.18 -4.1 147,680 - hisG DVU0114 0.21 -4.7 147,760 + hisG DVU0114 0.30 -1.7 147,821 + hisG DVU0114 0.37 -2.0 147,821 + hisG DVU0114 0.37 -3.4 147,828 - hisG DVU0114 0.38 -2.9 147,829 - hisG DVU0114 0.38 -3.5 147,829 - hisG DVU0114 0.38 -2.1 147,877 - hisG DVU0114 0.43 -2.4 147,894 - hisG DVU0114 0.45 -2.6 147,897 - hisG DVU0114 0.46 -1.8 147,946 + hisG DVU0114 0.51 -3.5 148,020 - hisG DVU0114 0.60 -0.2 148,071 + hisG DVU0114 0.65 -1.8 148,124 - hisG DVU0114 0.71 -0.9 148,172 - hisG DVU0114 0.77 -2.5 148,221 + hisG DVU0114 0.82 -0.7 148,280 + hisG DVU0114 0.89 -1.5 148,311 + -1.6 148,327 + -1.2 148,341 - -2.8 148,355 + -5.2 148,355 + -3.3 148,385 + -0.9 148,471 + -0.7 148,495 - -3.5 148,577 + aroE DVU0115 0.11 -3.1 149,321 + -4.4 149,321 + -4.2 149,321 + -5.2 149,322 + -2.0 149,324 - -3.1 149,329 - -3.3 149,374 - -0.1 149,375 - -1.7 149,389 + -0.2 149,436 + -0.6 149,436 + +0.9 149,499 - -0.4 149,535 + +0.2 149,563 + +0.4 149,571 - +0.6 149,595 + +1.9 149,608 + -0.2 149,637 + +0.5 149,676 + -2.4 149,821 + +0.1 149,892 - DVU0116 0.16 +0.0 149,901 + DVU0116 0.17 -0.2 149,918 + DVU0116 0.18 -0.9 149,966 - DVU0116 0.23 +0.9 150,035 + DVU0116 0.29 -1.2 150,195 + DVU0116 0.44 +0.3 150,195 + DVU0116 0.44 -0.9 150,195 + DVU0116 0.44 +0.0 150,195 + DVU0116 0.44 -0.4 150,196 + DVU0116 0.44 +0.1 150,197 + DVU0116 0.44 -0.3 150,203 - DVU0116 0.45 -0.0 150,217 - DVU0116 0.46 +0.1 150,219 - DVU0116 0.46 +0.6 150,248 + DVU0116 0.49 -0.1
Or see this region's nucleotide sequence