Experiment: MoLS4 with Spermidine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt relA and appA are separated by 3 nucleotides appA and DVU2085 are separated by 232 nucleotides
DVU2083: relA - GTP pyrophosphokinase (TIGR), at 2,168,573 to 2,170,726
relA
DVU2084: appA - oligopeptide-binding protein, putative (TIGR), at 2,170,730 to 2,172,427
appA
DVU2085: DVU2085 - Snf2 family protein (TIGR), at 2,172,660 to 2,175,875
DVU2085
Position (kb)
2170
2171
2172
2173 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2170.007 kb on - strand, within relA at 2170.467 kb on + strand, within relA at 2170.499 kb on - strand, within relA at 2170.598 kb on - strand at 2170.598 kb on - strand at 2170.690 kb on - strand at 2170.741 kb on - strand at 2170.905 kb on - strand, within appA at 2170.935 kb on - strand, within appA at 2170.965 kb on + strand, within appA at 2170.990 kb on - strand, within appA at 2171.035 kb on + strand, within appA at 2171.044 kb on + strand, within appA at 2171.046 kb on + strand, within appA at 2171.091 kb on - strand, within appA at 2171.144 kb on + strand, within appA at 2171.341 kb on + strand, within appA at 2171.341 kb on + strand, within appA at 2171.341 kb on + strand, within appA at 2171.341 kb on - strand, within appA at 2171.349 kb on - strand, within appA at 2171.349 kb on - strand, within appA at 2171.351 kb on + strand, within appA at 2171.355 kb on + strand, within appA at 2171.426 kb on - strand, within appA at 2171.582 kb on + strand, within appA at 2171.591 kb on - strand, within appA at 2171.664 kb on + strand, within appA at 2171.669 kb on + strand, within appA at 2171.934 kb on + strand, within appA at 2171.983 kb on + strand, within appA at 2172.016 kb on + strand, within appA at 2172.024 kb on - strand, within appA at 2172.042 kb on + strand, within appA at 2172.050 kb on - strand, within appA at 2172.095 kb on - strand, within appA at 2172.095 kb on - strand, within appA at 2172.095 kb on - strand, within appA at 2172.129 kb on - strand, within appA at 2172.134 kb on - strand, within appA at 2172.322 kb on + strand at 2172.474 kb on - strand at 2172.511 kb on + strand at 2172.511 kb on + strand at 2172.519 kb on - strand at 2172.519 kb on - strand at 2172.560 kb on - strand at 2172.670 kb on - strand at 2172.675 kb on - strand at 2172.865 kb on + strand at 2172.873 kb on - strand at 2172.983 kb on + strand, within DVU2085 at 2172.991 kb on - strand, within DVU2085 at 2173.038 kb on + strand, within DVU2085 at 2173.239 kb on - strand, within DVU2085 at 2173.251 kb on - strand, within DVU2085 at 2173.283 kb on - strand, within DVU2085 at 2173.407 kb on - strand, within DVU2085
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with Spermidine (N) remove 2,170,007 - relA DVU2083 0.67 -1.5 2,170,467 + relA DVU2083 0.88 -4.0 2,170,499 - relA DVU2083 0.89 +0.3 2,170,598 - -2.1 2,170,598 - -2.0 2,170,690 - -2.3 2,170,741 - -0.6 2,170,905 - appA DVU2084 0.10 -1.8 2,170,935 - appA DVU2084 0.12 +0.7 2,170,965 + appA DVU2084 0.14 -1.0 2,170,990 - appA DVU2084 0.15 -0.5 2,171,035 + appA DVU2084 0.18 -2.7 2,171,044 + appA DVU2084 0.18 -1.6 2,171,046 + appA DVU2084 0.19 -0.7 2,171,091 - appA DVU2084 0.21 -1.8 2,171,144 + appA DVU2084 0.24 +0.2 2,171,341 + appA DVU2084 0.36 -1.7 2,171,341 + appA DVU2084 0.36 -1.0 2,171,341 + appA DVU2084 0.36 -0.9 2,171,341 - appA DVU2084 0.36 -1.2 2,171,349 - appA DVU2084 0.36 -0.9 2,171,349 - appA DVU2084 0.36 -0.5 2,171,351 + appA DVU2084 0.37 -0.6 2,171,355 + appA DVU2084 0.37 -0.6 2,171,426 - appA DVU2084 0.41 -1.0 2,171,582 + appA DVU2084 0.50 -0.8 2,171,591 - appA DVU2084 0.51 -0.6 2,171,664 + appA DVU2084 0.55 -1.8 2,171,669 + appA DVU2084 0.55 -1.0 2,171,934 + appA DVU2084 0.71 -0.9 2,171,983 + appA DVU2084 0.74 -1.2 2,172,016 + appA DVU2084 0.76 -0.5 2,172,024 - appA DVU2084 0.76 +0.5 2,172,042 + appA DVU2084 0.77 -0.8 2,172,050 - appA DVU2084 0.78 -0.5 2,172,095 - appA DVU2084 0.80 -1.5 2,172,095 - appA DVU2084 0.80 -0.9 2,172,095 - appA DVU2084 0.80 -1.0 2,172,129 - appA DVU2084 0.82 -0.7 2,172,134 - appA DVU2084 0.83 -2.0 2,172,322 + -1.7 2,172,474 - -0.2 2,172,511 + +0.2 2,172,511 + +0.0 2,172,519 - -0.5 2,172,519 - -0.1 2,172,560 - -1.4 2,172,670 - -1.8 2,172,675 - -2.1 2,172,865 + -2.6 2,172,873 - -1.8 2,172,983 + DVU2085 0.10 -1.6 2,172,991 - DVU2085 0.10 +0.6 2,173,038 + DVU2085 0.12 -3.0 2,173,239 - DVU2085 0.18 -3.4 2,173,251 - DVU2085 0.18 -1.5 2,173,283 - DVU2085 0.19 -2.3 2,173,407 - DVU2085 0.23 -1.1
Or see this region's nucleotide sequence