Experiment: BG11 with Chloride 250 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt trpC and phdD are separated by 76 nucleotides phdD and Synpcc7942_1199 are separated by 58 nucleotides Synpcc7942_1199 and Synpcc7942_1200 are separated by 40 nucleotides
Synpcc7942_1197: trpC - indole-3-glycerol-phosphate synthase, at 1,224,841 to 1,225,728
trpC
Synpcc7942_1198: phdD - dihydrolipoamide dehydrogenase, at 1,225,805 to 1,227,244
phdD
Synpcc7942_1199: Synpcc7942_1199 - probable tRNA/rRNA methyltransferase, at 1,227,303 to 1,228,007
_1199
Synpcc7942_1200: Synpcc7942_1200 - hypothetical protein, at 1,228,048 to 1,229,061
_1200
Position (kb)
1225
1226
1227
1228 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1224.839 kb on + strand at 1224.839 kb on + strand at 1224.842 kb on - strand at 1224.846 kb on - strand at 1224.846 kb on - strand at 1224.847 kb on - strand at 1224.847 kb on - strand at 1224.853 kb on - strand at 1224.871 kb on + strand at 1225.738 kb on + strand at 1225.795 kb on + strand at 1225.832 kb on - strand at 1227.257 kb on - strand at 1227.333 kb on + strand at 1227.333 kb on + strand at 1227.363 kb on - strand at 1227.422 kb on + strand, within Synpcc7942_1199 at 1227.422 kb on + strand, within Synpcc7942_1199 at 1227.422 kb on + strand, within Synpcc7942_1199 at 1227.427 kb on + strand, within Synpcc7942_1199 at 1227.430 kb on - strand, within Synpcc7942_1199 at 1227.431 kb on - strand, within Synpcc7942_1199 at 1227.431 kb on - strand, within Synpcc7942_1199 at 1227.434 kb on + strand, within Synpcc7942_1199 at 1227.442 kb on - strand, within Synpcc7942_1199 at 1227.442 kb on - strand, within Synpcc7942_1199 at 1227.479 kb on + strand, within Synpcc7942_1199 at 1227.482 kb on - strand, within Synpcc7942_1199 at 1227.482 kb on - strand, within Synpcc7942_1199 at 1227.513 kb on - strand, within Synpcc7942_1199 at 1227.537 kb on + strand, within Synpcc7942_1199 at 1227.543 kb on - strand, within Synpcc7942_1199 at 1227.551 kb on + strand, within Synpcc7942_1199 at 1227.557 kb on + strand, within Synpcc7942_1199 at 1227.559 kb on - strand, within Synpcc7942_1199 at 1227.564 kb on + strand, within Synpcc7942_1199 at 1227.569 kb on + strand, within Synpcc7942_1199 at 1227.572 kb on - strand, within Synpcc7942_1199 at 1227.618 kb on + strand, within Synpcc7942_1199 at 1227.625 kb on + strand, within Synpcc7942_1199 at 1227.641 kb on + strand, within Synpcc7942_1199 at 1227.694 kb on + strand, within Synpcc7942_1199 at 1227.755 kb on + strand, within Synpcc7942_1199 at 1227.763 kb on - strand, within Synpcc7942_1199 at 1227.783 kb on - strand, within Synpcc7942_1199 at 1227.785 kb on + strand, within Synpcc7942_1199 at 1227.790 kb on + strand, within Synpcc7942_1199 at 1227.800 kb on + strand, within Synpcc7942_1199 at 1227.800 kb on + strand, within Synpcc7942_1199 at 1227.808 kb on - strand, within Synpcc7942_1199 at 1227.808 kb on - strand, within Synpcc7942_1199 at 1227.834 kb on - strand, within Synpcc7942_1199 at 1227.834 kb on - strand, within Synpcc7942_1199 at 1227.839 kb on + strand, within Synpcc7942_1199 at 1227.839 kb on + strand, within Synpcc7942_1199 at 1227.928 kb on + strand, within Synpcc7942_1199 at 1227.990 kb on - strand at 1227.990 kb on - strand at 1227.993 kb on - strand at 1228.026 kb on - strand at 1228.026 kb on - strand at 1228.026 kb on - strand at 1228.026 kb on - strand at 1228.079 kb on - strand at 1228.082 kb on + strand at 1228.082 kb on + strand at 1228.082 kb on + strand at 1228.082 kb on + strand at 1228.082 kb on + strand at 1228.084 kb on - strand at 1228.084 kb on - strand at 1228.090 kb on - strand at 1228.090 kb on - strand at 1228.092 kb on - strand at 1228.116 kb on + strand at 1228.132 kb on + strand at 1228.145 kb on - strand at 1228.234 kb on + strand, within Synpcc7942_1200
Per-strain Table
Position Strand Gene LocusTag Fraction BG11 with Chloride 250 mM remove 1,224,839 + -0.3 1,224,839 + +0.2 1,224,842 - +1.0 1,224,846 - +2.3 1,224,846 - -0.9 1,224,847 - +1.5 1,224,847 - +1.2 1,224,853 - -0.8 1,224,871 + -0.4 1,225,738 + -0.5 1,225,795 + -1.4 1,225,832 - -0.4 1,227,257 - -1.1 1,227,333 + +1.0 1,227,333 + -0.4 1,227,363 - +0.0 1,227,422 + Synpcc7942_1199 0.17 +1.4 1,227,422 + Synpcc7942_1199 0.17 -0.4 1,227,422 + Synpcc7942_1199 0.17 +1.2 1,227,427 + Synpcc7942_1199 0.18 +0.8 1,227,430 - Synpcc7942_1199 0.18 +1.1 1,227,431 - Synpcc7942_1199 0.18 +0.9 1,227,431 - Synpcc7942_1199 0.18 +1.4 1,227,434 + Synpcc7942_1199 0.19 +0.6 1,227,442 - Synpcc7942_1199 0.20 +0.7 1,227,442 - Synpcc7942_1199 0.20 -0.4 1,227,479 + Synpcc7942_1199 0.25 -0.6 1,227,482 - Synpcc7942_1199 0.25 +0.2 1,227,482 - Synpcc7942_1199 0.25 +2.4 1,227,513 - Synpcc7942_1199 0.30 +2.2 1,227,537 + Synpcc7942_1199 0.33 -0.2 1,227,543 - Synpcc7942_1199 0.34 -0.3 1,227,551 + Synpcc7942_1199 0.35 +0.4 1,227,557 + Synpcc7942_1199 0.36 +0.2 1,227,559 - Synpcc7942_1199 0.36 -0.2 1,227,564 + Synpcc7942_1199 0.37 +0.7 1,227,569 + Synpcc7942_1199 0.38 +0.2 1,227,572 - Synpcc7942_1199 0.38 +0.4 1,227,618 + Synpcc7942_1199 0.45 +1.8 1,227,625 + Synpcc7942_1199 0.46 -0.6 1,227,641 + Synpcc7942_1199 0.48 -0.1 1,227,694 + Synpcc7942_1199 0.55 +0.3 1,227,755 + Synpcc7942_1199 0.64 +0.7 1,227,763 - Synpcc7942_1199 0.65 -1.0 1,227,783 - Synpcc7942_1199 0.68 +1.4 1,227,785 + Synpcc7942_1199 0.68 +0.3 1,227,790 + Synpcc7942_1199 0.69 -0.0 1,227,800 + Synpcc7942_1199 0.70 +1.0 1,227,800 + Synpcc7942_1199 0.70 +0.7 1,227,808 - Synpcc7942_1199 0.72 +2.0 1,227,808 - Synpcc7942_1199 0.72 +2.9 1,227,834 - Synpcc7942_1199 0.75 +0.4 1,227,834 - Synpcc7942_1199 0.75 +0.8 1,227,839 + Synpcc7942_1199 0.76 +0.0 1,227,839 + Synpcc7942_1199 0.76 +0.4 1,227,928 + Synpcc7942_1199 0.89 +0.1 1,227,990 - +0.7 1,227,990 - +0.3 1,227,993 - +0.8 1,228,026 - +0.4 1,228,026 - +0.3 1,228,026 - +1.5 1,228,026 - +0.3 1,228,079 - +2.2 1,228,082 + -0.4 1,228,082 + -3.0 1,228,082 + +0.2 1,228,082 + +2.4 1,228,082 + -2.7 1,228,084 - +0.2 1,228,084 - -3.0 1,228,090 - -0.2 1,228,090 - -2.9 1,228,092 - -0.4 1,228,116 + -1.6 1,228,132 + +1.0 1,228,145 - -0.8 1,228,234 + Synpcc7942_1200 0.18 -2.2
Or see this region's nucleotide sequence