Experiment: BG11 with Chloride 250 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt petJ and apcD are separated by 181 nucleotides apcD and rumA are separated by 144 nucleotides rumA and Synpcc7942_0242 are separated by 311 nucleotides
Synpcc7942_0239: petJ - cytochrome C6 soluble cytochrome f, at 234,331 to 234,669
petJ
Synpcc7942_0240: apcD - hypothetical protein, at 234,851 to 235,342
apcD
Synpcc7942_0241: rumA - 23S rRNA (uracil-5-)-methyltransferase RumA, at 235,487 to 236,854
rumA
Synpcc7942_0242: Synpcc7942_0242 - K+-dependent Na+/Ca+ exchanger related-protein, at 237,166 to 238,275
_0242
Position (kb) 235
236
237 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 234.487 kb on + strand, within petJ at 234.487 kb on + strand, within petJ at 234.495 kb on - strand, within petJ at 234.563 kb on + strand, within petJ at 234.581 kb on - strand, within petJ at 234.581 kb on - strand, within petJ at 234.614 kb on + strand, within petJ at 234.614 kb on + strand, within petJ at 234.614 kb on + strand, within petJ at 234.620 kb on + strand, within petJ at 234.646 kb on + strand at 234.646 kb on + strand at 234.646 kb on + strand at 234.646 kb on + strand at 234.646 kb on + strand at 234.654 kb on - strand at 234.654 kb on - strand at 234.654 kb on - strand at 234.794 kb on + strand at 234.795 kb on - strand at 234.839 kb on + strand at 234.848 kb on + strand at 234.849 kb on + strand at 234.857 kb on - strand at 234.915 kb on + strand, within apcD at 234.917 kb on + strand, within apcD at 234.920 kb on + strand, within apcD at 234.920 kb on - strand, within apcD at 234.923 kb on - strand, within apcD at 234.923 kb on - strand, within apcD at 234.925 kb on + strand, within apcD at 234.925 kb on - strand, within apcD at 234.982 kb on + strand, within apcD at 234.992 kb on - strand, within apcD at 235.008 kb on + strand, within apcD at 235.013 kb on + strand, within apcD at 235.013 kb on + strand, within apcD at 235.018 kb on + strand, within apcD at 235.021 kb on - strand, within apcD at 235.026 kb on - strand, within apcD at 235.026 kb on - strand, within apcD at 235.047 kb on - strand, within apcD at 235.049 kb on + strand, within apcD at 235.057 kb on - strand, within apcD at 235.057 kb on - strand, within apcD at 235.103 kb on - strand, within apcD at 235.107 kb on - strand, within apcD at 235.109 kb on + strand, within apcD at 235.113 kb on - strand, within apcD at 235.114 kb on + strand, within apcD at 235.120 kb on + strand, within apcD at 235.142 kb on - strand, within apcD at 235.181 kb on + strand, within apcD at 235.220 kb on - strand, within apcD at 235.220 kb on - strand, within apcD at 235.263 kb on + strand, within apcD at 235.276 kb on - strand, within apcD at 235.335 kb on - strand at 235.374 kb on - strand at 235.376 kb on - strand at 235.384 kb on - strand at 235.415 kb on - strand at 235.447 kb on - strand at 235.447 kb on - strand at 235.468 kb on - strand at 235.473 kb on + strand at 235.478 kb on - strand at 235.491 kb on - strand at 235.550 kb on + strand at 235.550 kb on + strand at 235.558 kb on - strand at 235.576 kb on - strand at 235.588 kb on - strand at 235.593 kb on - strand at 235.608 kb on + strand at 235.608 kb on + strand at 235.666 kb on - strand, within rumA at 235.685 kb on + strand at 235.685 kb on + strand, within rumA at 235.693 kb on - strand, within rumA at 235.693 kb on - strand, within rumA at 235.715 kb on - strand, within rumA at 235.753 kb on + strand, within rumA at 235.760 kb on + strand, within rumA at 235.765 kb on + strand, within rumA at 235.765 kb on + strand, within rumA at 235.770 kb on + strand, within rumA at 235.775 kb on + strand, within rumA at 235.775 kb on + strand, within rumA at 235.778 kb on - strand, within rumA at 235.783 kb on - strand, within rumA at 235.811 kb on + strand, within rumA at 235.817 kb on - strand, within rumA at 235.821 kb on + strand, within rumA at 235.879 kb on - strand, within rumA at 235.879 kb on - strand, within rumA at 235.881 kb on + strand, within rumA at 235.884 kb on - strand, within rumA at 235.889 kb on + strand, within rumA at 235.927 kb on + strand, within rumA at 235.936 kb on - strand, within rumA at 235.968 kb on - strand, within rumA at 235.979 kb on - strand, within rumA at 235.979 kb on - strand, within rumA at 235.983 kb on + strand, within rumA at 236.006 kb on + strand at 236.023 kb on + strand, within rumA at 236.023 kb on + strand at 236.023 kb on + strand, within rumA at 236.023 kb on + strand, within rumA at 236.031 kb on - strand, within rumA at 236.031 kb on - strand, within rumA at 236.042 kb on + strand, within rumA at 236.060 kb on - strand, within rumA at 236.074 kb on - strand, within rumA at 236.079 kb on + strand, within rumA at 236.102 kb on - strand, within rumA at 236.124 kb on - strand, within rumA at 236.132 kb on - strand, within rumA at 236.139 kb on + strand, within rumA at 236.161 kb on + strand, within rumA at 236.176 kb on + strand, within rumA at 236.194 kb on - strand, within rumA at 236.205 kb on + strand, within rumA at 236.205 kb on + strand, within rumA at 236.220 kb on + strand, within rumA at 236.233 kb on + strand, within rumA at 236.233 kb on + strand, within rumA at 236.246 kb on - strand, within rumA at 236.265 kb on - strand, within rumA at 236.292 kb on + strand, within rumA at 236.302 kb on + strand, within rumA at 236.310 kb on - strand, within rumA at 236.356 kb on + strand, within rumA at 236.364 kb on - strand, within rumA at 236.429 kb on + strand, within rumA at 236.434 kb on + strand, within rumA at 236.437 kb on - strand, within rumA at 236.442 kb on - strand, within rumA at 236.451 kb on + strand, within rumA at 236.458 kb on - strand, within rumA at 236.458 kb on - strand at 236.459 kb on - strand, within rumA at 236.459 kb on - strand, within rumA at 236.469 kb on - strand, within rumA at 236.470 kb on - strand, within rumA at 236.474 kb on + strand, within rumA at 236.476 kb on + strand, within rumA at 236.481 kb on + strand, within rumA at 236.482 kb on - strand, within rumA at 236.486 kb on + strand, within rumA at 236.486 kb on + strand, within rumA at 236.494 kb on - strand, within rumA at 236.494 kb on - strand, within rumA at 236.494 kb on - strand at 236.530 kb on + strand, within rumA at 236.537 kb on + strand, within rumA at 236.587 kb on + strand, within rumA at 236.636 kb on - strand, within rumA at 236.663 kb on - strand, within rumA at 236.672 kb on + strand, within rumA at 236.673 kb on - strand, within rumA at 236.702 kb on + strand, within rumA at 236.721 kb on + strand at 236.721 kb on - strand at 236.734 kb on - strand at 236.734 kb on - strand at 236.734 kb on - strand at 236.734 kb on - strand at 236.734 kb on - strand at 236.741 kb on + strand at 236.749 kb on - strand at 236.750 kb on - strand at 236.757 kb on - strand at 236.790 kb on + strand at 236.798 kb on - strand at 236.822 kb on - strand at 236.836 kb on + strand at 236.849 kb on + strand at 236.849 kb on + strand at 236.849 kb on + strand at 236.915 kb on - strand at 236.918 kb on + strand at 236.918 kb on + strand at 236.926 kb on - strand at 236.939 kb on + strand at 236.939 kb on + strand at 236.941 kb on - strand at 236.947 kb on - strand at 236.947 kb on - strand at 236.965 kb on + strand at 236.965 kb on + strand at 236.975 kb on + strand at 236.978 kb on - strand at 236.983 kb on - strand at 237.013 kb on + strand at 237.016 kb on - strand at 237.029 kb on + strand at 237.029 kb on + strand at 237.029 kb on + strand at 237.029 kb on - strand at 237.031 kb on + strand at 237.107 kb on - strand at 237.115 kb on + strand at 237.115 kb on + strand at 237.163 kb on - strand at 237.164 kb on + strand at 237.204 kb on + strand at 237.223 kb on - strand at 237.237 kb on - strand at 237.258 kb on - strand at 237.287 kb on + strand, within Synpcc7942_0242 at 237.315 kb on - strand, within Synpcc7942_0242 at 237.392 kb on + strand, within Synpcc7942_0242 at 237.489 kb on + strand, within Synpcc7942_0242 at 237.489 kb on + strand, within Synpcc7942_0242 at 237.498 kb on - strand, within Synpcc7942_0242 at 237.500 kb on + strand, within Synpcc7942_0242 at 237.542 kb on + strand, within Synpcc7942_0242 at 237.550 kb on - strand, within Synpcc7942_0242 at 237.557 kb on + strand, within Synpcc7942_0242 at 237.581 kb on - strand at 237.595 kb on - strand, within Synpcc7942_0242 at 237.597 kb on + strand, within Synpcc7942_0242 at 237.619 kb on + strand, within Synpcc7942_0242 at 237.634 kb on - strand, within Synpcc7942_0242 at 237.636 kb on - strand, within Synpcc7942_0242 at 237.645 kb on + strand, within Synpcc7942_0242 at 237.652 kb on + strand, within Synpcc7942_0242 at 237.660 kb on - strand, within Synpcc7942_0242 at 237.680 kb on + strand, within Synpcc7942_0242 at 237.695 kb on - strand, within Synpcc7942_0242 at 237.706 kb on - strand at 237.709 kb on + strand, within Synpcc7942_0242 at 237.717 kb on - strand, within Synpcc7942_0242 at 237.741 kb on + strand, within Synpcc7942_0242 at 237.742 kb on + strand, within Synpcc7942_0242 at 237.782 kb on - strand, within Synpcc7942_0242 at 237.791 kb on - strand, within Synpcc7942_0242 at 237.825 kb on - strand, within Synpcc7942_0242 at 237.839 kb on + strand, within Synpcc7942_0242 at 237.839 kb on + strand, within Synpcc7942_0242
Per-strain Table
Position Strand Gene LocusTag Fraction BG11 with Chloride 250 mM remove 234,487 + petJ Synpcc7942_0239 0.46 -1.5 234,487 + petJ Synpcc7942_0239 0.46 -0.6 234,495 - petJ Synpcc7942_0239 0.48 -0.2 234,563 + petJ Synpcc7942_0239 0.68 +0.1 234,581 - petJ Synpcc7942_0239 0.74 -0.9 234,581 - petJ Synpcc7942_0239 0.74 +0.3 234,614 + petJ Synpcc7942_0239 0.83 -2.4 234,614 + petJ Synpcc7942_0239 0.83 -0.7 234,614 + petJ Synpcc7942_0239 0.83 -2.5 234,620 + petJ Synpcc7942_0239 0.85 +1.1 234,646 + -0.4 234,646 + -1.2 234,646 + -0.2 234,646 + +1.0 234,646 + -0.6 234,654 - -0.2 234,654 - +0.4 234,654 - +0.1 234,794 + -1.3 234,795 - +0.8 234,839 + +0.3 234,848 + +1.0 234,849 + -2.3 234,857 - +0.4 234,915 + apcD Synpcc7942_0240 0.13 +0.3 234,917 + apcD Synpcc7942_0240 0.13 -1.2 234,920 + apcD Synpcc7942_0240 0.14 +1.1 234,920 - apcD Synpcc7942_0240 0.14 -1.0 234,923 - apcD Synpcc7942_0240 0.15 +0.3 234,923 - apcD Synpcc7942_0240 0.15 +1.0 234,925 + apcD Synpcc7942_0240 0.15 -1.1 234,925 - apcD Synpcc7942_0240 0.15 -1.3 234,982 + apcD Synpcc7942_0240 0.27 -1.2 234,992 - apcD Synpcc7942_0240 0.29 -2.1 235,008 + apcD Synpcc7942_0240 0.32 +0.9 235,013 + apcD Synpcc7942_0240 0.33 -1.8 235,013 + apcD Synpcc7942_0240 0.33 -0.9 235,018 + apcD Synpcc7942_0240 0.34 +0.8 235,021 - apcD Synpcc7942_0240 0.35 -0.5 235,026 - apcD Synpcc7942_0240 0.36 +0.4 235,026 - apcD Synpcc7942_0240 0.36 -0.6 235,047 - apcD Synpcc7942_0240 0.40 +0.6 235,049 + apcD Synpcc7942_0240 0.40 -0.9 235,057 - apcD Synpcc7942_0240 0.42 -0.5 235,057 - apcD Synpcc7942_0240 0.42 +0.4 235,103 - apcD Synpcc7942_0240 0.51 -1.0 235,107 - apcD Synpcc7942_0240 0.52 -1.1 235,109 + apcD Synpcc7942_0240 0.52 -0.6 235,113 - apcD Synpcc7942_0240 0.53 -1.2 235,114 + apcD Synpcc7942_0240 0.53 -1.9 235,120 + apcD Synpcc7942_0240 0.55 +0.1 235,142 - apcD Synpcc7942_0240 0.59 +0.8 235,181 + apcD Synpcc7942_0240 0.67 +0.9 235,220 - apcD Synpcc7942_0240 0.75 +1.2 235,220 - apcD Synpcc7942_0240 0.75 +0.8 235,263 + apcD Synpcc7942_0240 0.84 +0.2 235,276 - apcD Synpcc7942_0240 0.86 -0.4 235,335 - -0.5 235,374 - -1.2 235,376 - -0.9 235,384 - +1.3 235,415 - +0.1 235,447 - +0.4 235,447 - -0.1 235,468 - -0.9 235,473 + +0.7 235,478 - +0.5 235,491 - -0.2 235,550 + +0.6 235,550 + +1.0 235,558 - +0.1 235,576 - +1.3 235,588 - +0.4 235,593 - -0.7 235,608 + +0.7 235,608 + -0.6 235,666 - rumA Synpcc7942_0241 0.13 +0.7 235,685 + +1.1 235,685 + rumA Synpcc7942_0241 0.14 +1.1 235,693 - rumA Synpcc7942_0241 0.15 +0.8 235,693 - rumA Synpcc7942_0241 0.15 +0.4 235,715 - rumA Synpcc7942_0241 0.17 +0.9 235,753 + rumA Synpcc7942_0241 0.19 -0.3 235,760 + rumA Synpcc7942_0241 0.20 +1.6 235,765 + rumA Synpcc7942_0241 0.20 +0.3 235,765 + rumA Synpcc7942_0241 0.20 +0.2 235,770 + rumA Synpcc7942_0241 0.21 +0.0 235,775 + rumA Synpcc7942_0241 0.21 +1.4 235,775 + rumA Synpcc7942_0241 0.21 -0.1 235,778 - rumA Synpcc7942_0241 0.21 -1.3 235,783 - rumA Synpcc7942_0241 0.22 -0.4 235,811 + rumA Synpcc7942_0241 0.24 -0.5 235,817 - rumA Synpcc7942_0241 0.24 +0.4 235,821 + rumA Synpcc7942_0241 0.24 +1.6 235,879 - rumA Synpcc7942_0241 0.29 +1.2 235,879 - rumA Synpcc7942_0241 0.29 +1.1 235,881 + rumA Synpcc7942_0241 0.29 +0.8 235,884 - rumA Synpcc7942_0241 0.29 -0.4 235,889 + rumA Synpcc7942_0241 0.29 +2.6 235,927 + rumA Synpcc7942_0241 0.32 -0.6 235,936 - rumA Synpcc7942_0241 0.33 +1.1 235,968 - rumA Synpcc7942_0241 0.35 +0.8 235,979 - rumA Synpcc7942_0241 0.36 +2.6 235,979 - rumA Synpcc7942_0241 0.36 -1.1 235,983 + rumA Synpcc7942_0241 0.36 +1.3 236,006 + +3.0 236,023 + rumA Synpcc7942_0241 0.39 +0.1 236,023 + +1.4 236,023 + rumA Synpcc7942_0241 0.39 -0.2 236,023 + rumA Synpcc7942_0241 0.39 +1.5 236,031 - rumA Synpcc7942_0241 0.40 -0.4 236,031 - rumA Synpcc7942_0241 0.40 +1.7 236,042 + rumA Synpcc7942_0241 0.41 +1.1 236,060 - rumA Synpcc7942_0241 0.42 +1.0 236,074 - rumA Synpcc7942_0241 0.43 +1.4 236,079 + rumA Synpcc7942_0241 0.43 -2.3 236,102 - rumA Synpcc7942_0241 0.45 -0.1 236,124 - rumA Synpcc7942_0241 0.47 +2.4 236,132 - rumA Synpcc7942_0241 0.47 +0.1 236,139 + rumA Synpcc7942_0241 0.48 +0.4 236,161 + rumA Synpcc7942_0241 0.49 +1.3 236,176 + rumA Synpcc7942_0241 0.50 +0.2 236,194 - rumA Synpcc7942_0241 0.52 +0.3 236,205 + rumA Synpcc7942_0241 0.52 +0.9 236,205 + rumA Synpcc7942_0241 0.52 +0.2 236,220 + rumA Synpcc7942_0241 0.54 +0.7 236,233 + rumA Synpcc7942_0241 0.55 +3.7 236,233 + rumA Synpcc7942_0241 0.55 +2.3 236,246 - rumA Synpcc7942_0241 0.55 +2.0 236,265 - rumA Synpcc7942_0241 0.57 -0.5 236,292 + rumA Synpcc7942_0241 0.59 +1.1 236,302 + rumA Synpcc7942_0241 0.60 +1.4 236,310 - rumA Synpcc7942_0241 0.60 -0.1 236,356 + rumA Synpcc7942_0241 0.64 +0.5 236,364 - rumA Synpcc7942_0241 0.64 +1.4 236,429 + rumA Synpcc7942_0241 0.69 +0.1 236,434 + rumA Synpcc7942_0241 0.69 +1.0 236,437 - rumA Synpcc7942_0241 0.69 +0.3 236,442 - rumA Synpcc7942_0241 0.70 -0.2 236,451 + rumA Synpcc7942_0241 0.70 +1.2 236,458 - rumA Synpcc7942_0241 0.71 +1.5 236,458 - +0.4 236,459 - rumA Synpcc7942_0241 0.71 +0.1 236,459 - rumA Synpcc7942_0241 0.71 +1.1 236,469 - rumA Synpcc7942_0241 0.72 -1.2 236,470 - rumA Synpcc7942_0241 0.72 +0.5 236,474 + rumA Synpcc7942_0241 0.72 +0.5 236,476 + rumA Synpcc7942_0241 0.72 -1.0 236,481 + rumA Synpcc7942_0241 0.73 -0.7 236,482 - rumA Synpcc7942_0241 0.73 +0.8 236,486 + rumA Synpcc7942_0241 0.73 -1.0 236,486 + rumA Synpcc7942_0241 0.73 -0.1 236,494 - rumA Synpcc7942_0241 0.74 +0.8 236,494 - rumA Synpcc7942_0241 0.74 -0.1 236,494 - +0.1 236,530 + rumA Synpcc7942_0241 0.76 +0.8 236,537 + rumA Synpcc7942_0241 0.77 +1.6 236,587 + rumA Synpcc7942_0241 0.80 +2.6 236,636 - rumA Synpcc7942_0241 0.84 -0.0 236,663 - rumA Synpcc7942_0241 0.86 +0.2 236,672 + rumA Synpcc7942_0241 0.87 +1.7 236,673 - rumA Synpcc7942_0241 0.87 -2.5 236,702 + rumA Synpcc7942_0241 0.89 +1.1 236,721 + +0.6 236,721 - +0.6 236,734 - +1.4 236,734 - +0.8 236,734 - -0.7 236,734 - -0.0 236,734 - -0.2 236,741 + -0.3 236,749 - +0.1 236,750 - +1.1 236,757 - +2.2 236,790 + +0.9 236,798 - +2.4 236,822 - +0.9 236,836 + -2.4 236,849 + +2.4 236,849 + -0.7 236,849 + -0.4 236,915 - -2.5 236,918 + +1.4 236,918 + +1.8 236,926 - +1.7 236,939 + -0.4 236,939 + -0.3 236,941 - -1.2 236,947 - -1.1 236,947 - -1.4 236,965 + +0.8 236,965 + -0.8 236,975 + -1.0 236,978 - -1.3 236,983 - +0.1 237,013 + -1.1 237,016 - -0.4 237,029 + +0.1 237,029 + +0.7 237,029 + -0.5 237,029 - -2.0 237,031 + +0.7 237,107 - -1.0 237,115 + +0.6 237,115 + -1.3 237,163 - -1.0 237,164 + -0.6 237,204 + +1.7 237,223 - +2.1 237,237 - -0.2 237,258 - +2.6 237,287 + Synpcc7942_0242 0.11 -0.4 237,315 - Synpcc7942_0242 0.13 +3.0 237,392 + Synpcc7942_0242 0.20 +1.8 237,489 + Synpcc7942_0242 0.29 +1.5 237,489 + Synpcc7942_0242 0.29 +1.4 237,498 - Synpcc7942_0242 0.30 +0.7 237,500 + Synpcc7942_0242 0.30 +1.8 237,542 + Synpcc7942_0242 0.34 +0.1 237,550 - Synpcc7942_0242 0.35 +1.1 237,557 + Synpcc7942_0242 0.35 +1.1 237,581 - +2.9 237,595 - Synpcc7942_0242 0.39 +1.6 237,597 + Synpcc7942_0242 0.39 +1.7 237,619 + Synpcc7942_0242 0.41 +1.8 237,634 - Synpcc7942_0242 0.42 +3.8 237,636 - Synpcc7942_0242 0.42 +0.8 237,645 + Synpcc7942_0242 0.43 +0.5 237,652 + Synpcc7942_0242 0.44 +2.3 237,660 - Synpcc7942_0242 0.45 +1.6 237,680 + Synpcc7942_0242 0.46 +2.2 237,695 - Synpcc7942_0242 0.48 +1.5 237,706 - +0.8 237,709 + Synpcc7942_0242 0.49 +0.6 237,717 - Synpcc7942_0242 0.50 -0.7 237,741 + Synpcc7942_0242 0.52 +1.5 237,742 + Synpcc7942_0242 0.52 +2.4 237,782 - Synpcc7942_0242 0.55 +1.6 237,791 - Synpcc7942_0242 0.56 +2.3 237,825 - Synpcc7942_0242 0.59 -1.2 237,839 + Synpcc7942_0242 0.61 +2.0 237,839 + Synpcc7942_0242 0.61 +1.8
Or see this region's nucleotide sequence