Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS26675 and BWI76_RS26680 are separated by 185 nucleotides BWI76_RS26680 and BWI76_RS26685 are separated by 71 nucleotides
BWI76_RS26675: BWI76_RS26675 - phosphoesterase PA-phosphatase-like protein, at 5,523,043 to 5,524,314
_RS26675
BWI76_RS26680: BWI76_RS26680 - 23S rRNA (adenine(2030)-N(6))-methyltransferase RlmJ, at 5,524,500 to 5,525,342
_RS26680
BWI76_RS26685: BWI76_RS26685 - glutathione-disulfide reductase, at 5,525,414 to 5,526,766
_RS26685
Position (kb) 5524
5525
5526 Strain fitness (log2 ratio) -2
-1
0
1 at 5523.526 kb on + strand, within BWI76_RS26675 at 5523.608 kb on + strand, within BWI76_RS26675 at 5523.647 kb on + strand, within BWI76_RS26675 at 5523.647 kb on + strand, within BWI76_RS26675 at 5523.647 kb on + strand, within BWI76_RS26675 at 5523.652 kb on + strand, within BWI76_RS26675 at 5523.655 kb on - strand, within BWI76_RS26675 at 5523.655 kb on - strand, within BWI76_RS26675 at 5523.698 kb on + strand, within BWI76_RS26675 at 5523.698 kb on + strand, within BWI76_RS26675 at 5523.706 kb on - strand, within BWI76_RS26675 at 5523.762 kb on + strand, within BWI76_RS26675 at 5523.820 kb on + strand, within BWI76_RS26675 at 5523.828 kb on - strand, within BWI76_RS26675 at 5523.916 kb on + strand, within BWI76_RS26675 at 5523.921 kb on - strand, within BWI76_RS26675 at 5523.997 kb on + strand, within BWI76_RS26675 at 5524.214 kb on + strand at 5524.397 kb on - strand at 5524.432 kb on + strand at 5524.442 kb on + strand at 5524.445 kb on - strand at 5524.512 kb on + strand at 5524.515 kb on - strand at 5524.538 kb on - strand at 5524.586 kb on - strand, within BWI76_RS26680 at 5524.633 kb on - strand, within BWI76_RS26680 at 5524.719 kb on - strand, within BWI76_RS26680 at 5524.760 kb on + strand, within BWI76_RS26680 at 5524.784 kb on + strand, within BWI76_RS26680 at 5524.827 kb on + strand, within BWI76_RS26680 at 5524.889 kb on + strand, within BWI76_RS26680 at 5524.938 kb on - strand, within BWI76_RS26680 at 5524.953 kb on - strand, within BWI76_RS26680 at 5525.527 kb on + strand at 5525.758 kb on - strand, within BWI76_RS26685 at 5525.845 kb on - strand, within BWI76_RS26685 at 5525.882 kb on - strand, within BWI76_RS26685 at 5526.091 kb on - strand, within BWI76_RS26685 at 5526.159 kb on + strand, within BWI76_RS26685 at 5526.170 kb on + strand, within BWI76_RS26685 at 5526.178 kb on - strand, within BWI76_RS26685
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 5,523,526 + BWI76_RS26675 0.38 +0.1 5,523,608 + BWI76_RS26675 0.44 -0.0 5,523,647 + BWI76_RS26675 0.47 -0.1 5,523,647 + BWI76_RS26675 0.47 +0.3 5,523,647 + BWI76_RS26675 0.47 -0.5 5,523,652 + BWI76_RS26675 0.48 -2.2 5,523,655 - BWI76_RS26675 0.48 -0.0 5,523,655 - BWI76_RS26675 0.48 -0.5 5,523,698 + BWI76_RS26675 0.51 -0.5 5,523,698 + BWI76_RS26675 0.51 +0.7 5,523,706 - BWI76_RS26675 0.52 +0.6 5,523,762 + BWI76_RS26675 0.57 +0.6 5,523,820 + BWI76_RS26675 0.61 +1.2 5,523,828 - BWI76_RS26675 0.62 -0.8 5,523,916 + BWI76_RS26675 0.69 -0.1 5,523,921 - BWI76_RS26675 0.69 -0.5 5,523,997 + BWI76_RS26675 0.75 -0.1 5,524,214 + -1.3 5,524,397 - -1.4 5,524,432 + +0.6 5,524,442 + +0.1 5,524,445 - +0.2 5,524,512 + -0.2 5,524,515 - +0.3 5,524,538 - -0.0 5,524,586 - BWI76_RS26680 0.10 -0.2 5,524,633 - BWI76_RS26680 0.16 +0.7 5,524,719 - BWI76_RS26680 0.26 +0.1 5,524,760 + BWI76_RS26680 0.31 +0.4 5,524,784 + BWI76_RS26680 0.34 +1.6 5,524,827 + BWI76_RS26680 0.39 -1.9 5,524,889 + BWI76_RS26680 0.46 +1.0 5,524,938 - BWI76_RS26680 0.52 +0.7 5,524,953 - BWI76_RS26680 0.54 +0.8 5,525,527 + +0.0 5,525,758 - BWI76_RS26685 0.25 +0.9 5,525,845 - BWI76_RS26685 0.32 +0.1 5,525,882 - BWI76_RS26685 0.35 -0.8 5,526,091 - BWI76_RS26685 0.50 +0.2 5,526,159 + BWI76_RS26685 0.55 -2.1 5,526,170 + BWI76_RS26685 0.56 -1.2 5,526,178 - BWI76_RS26685 0.56 -0.3
Or see this region's nucleotide sequence