Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS26045 and BWI76_RS26050 are separated by 67 nucleotides BWI76_RS26050 and BWI76_RS26055 are separated by 11 nucleotides BWI76_RS26055 and BWI76_RS26060 are separated by 89 nucleotides
BWI76_RS26045: BWI76_RS26045 - nitrite reductase small subunit, at 5,382,751 to 5,383,077
_RS26045
BWI76_RS26050: BWI76_RS26050 - nitrite transporter NirC, at 5,383,145 to 5,383,954
_RS26050
BWI76_RS26055: BWI76_RS26055 - siroheme synthase, at 5,383,966 to 5,385,339
_RS26055
BWI76_RS26060: BWI76_RS26060 - tryptophan--tRNA ligase, at 5,385,429 to 5,386,433
_RS26060
Position (kb) 5383
5384
5385
5386 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 5383.007 kb on - strand, within BWI76_RS26045 at 5383.017 kb on - strand, within BWI76_RS26045 at 5383.124 kb on + strand at 5383.182 kb on - strand at 5383.182 kb on - strand at 5383.254 kb on + strand, within BWI76_RS26050 at 5383.262 kb on - strand, within BWI76_RS26050 at 5383.346 kb on + strand, within BWI76_RS26050 at 5383.354 kb on - strand, within BWI76_RS26050 at 5383.436 kb on - strand, within BWI76_RS26050 at 5383.447 kb on - strand, within BWI76_RS26050 at 5383.629 kb on - strand, within BWI76_RS26050 at 5383.703 kb on + strand, within BWI76_RS26050 at 5383.765 kb on + strand, within BWI76_RS26050 at 5383.773 kb on - strand, within BWI76_RS26050 at 5383.773 kb on - strand, within BWI76_RS26050 at 5383.791 kb on + strand, within BWI76_RS26050 at 5383.791 kb on + strand, within BWI76_RS26050 at 5383.798 kb on - strand, within BWI76_RS26050 at 5383.827 kb on + strand, within BWI76_RS26050 at 5383.837 kb on + strand, within BWI76_RS26050 at 5384.040 kb on + strand at 5384.048 kb on - strand at 5384.048 kb on - strand at 5384.157 kb on + strand, within BWI76_RS26055 at 5384.279 kb on + strand, within BWI76_RS26055 at 5384.441 kb on - strand, within BWI76_RS26055 at 5384.653 kb on - strand, within BWI76_RS26055 at 5384.661 kb on - strand, within BWI76_RS26055 at 5384.737 kb on - strand, within BWI76_RS26055 at 5384.782 kb on + strand, within BWI76_RS26055 at 5384.790 kb on - strand, within BWI76_RS26055 at 5384.805 kb on - strand, within BWI76_RS26055 at 5384.904 kb on + strand, within BWI76_RS26055 at 5385.042 kb on + strand, within BWI76_RS26055 at 5385.042 kb on + strand, within BWI76_RS26055 at 5385.042 kb on + strand, within BWI76_RS26055 at 5385.050 kb on - strand, within BWI76_RS26055 at 5385.195 kb on - strand, within BWI76_RS26055 at 5385.216 kb on - strand at 5385.230 kb on + strand at 5385.238 kb on - strand at 5385.238 kb on - strand at 5385.304 kb on - strand at 5385.353 kb on + strand at 5385.427 kb on + strand at 5385.428 kb on - strand at 5385.440 kb on - strand at 5385.542 kb on - strand, within BWI76_RS26060 at 5385.542 kb on - strand, within BWI76_RS26060 at 5385.564 kb on + strand, within BWI76_RS26060 at 5385.610 kb on + strand, within BWI76_RS26060 at 5385.706 kb on - strand, within BWI76_RS26060 at 5385.867 kb on - strand, within BWI76_RS26060 at 5386.074 kb on - strand, within BWI76_RS26060 at 5386.074 kb on - strand, within BWI76_RS26060 at 5386.074 kb on - strand, within BWI76_RS26060 at 5386.190 kb on + strand, within BWI76_RS26060 at 5386.236 kb on - strand, within BWI76_RS26060 at 5386.258 kb on + strand, within BWI76_RS26060 at 5386.266 kb on - strand, within BWI76_RS26060 at 5386.266 kb on - strand, within BWI76_RS26060
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 5,383,007 - BWI76_RS26045 0.78 -0.1 5,383,017 - BWI76_RS26045 0.81 -0.0 5,383,124 + +0.1 5,383,182 - -0.0 5,383,182 - +0.1 5,383,254 + BWI76_RS26050 0.13 +1.3 5,383,262 - BWI76_RS26050 0.14 -1.2 5,383,346 + BWI76_RS26050 0.25 -1.8 5,383,354 - BWI76_RS26050 0.26 -0.5 5,383,436 - BWI76_RS26050 0.36 -0.1 5,383,447 - BWI76_RS26050 0.37 -1.7 5,383,629 - BWI76_RS26050 0.60 -0.6 5,383,703 + BWI76_RS26050 0.69 -0.8 5,383,765 + BWI76_RS26050 0.77 +0.2 5,383,773 - BWI76_RS26050 0.78 -0.3 5,383,773 - BWI76_RS26050 0.78 +0.1 5,383,791 + BWI76_RS26050 0.80 +0.0 5,383,791 + BWI76_RS26050 0.80 +0.1 5,383,798 - BWI76_RS26050 0.81 -0.3 5,383,827 + BWI76_RS26050 0.84 -1.8 5,383,837 + BWI76_RS26050 0.85 -0.1 5,384,040 + -3.6 5,384,048 - -2.3 5,384,048 - -1.7 5,384,157 + BWI76_RS26055 0.14 -3.6 5,384,279 + BWI76_RS26055 0.23 -2.2 5,384,441 - BWI76_RS26055 0.35 -1.3 5,384,653 - BWI76_RS26055 0.50 -2.5 5,384,661 - BWI76_RS26055 0.51 -2.1 5,384,737 - BWI76_RS26055 0.56 +0.2 5,384,782 + BWI76_RS26055 0.59 -4.0 5,384,790 - BWI76_RS26055 0.60 -3.5 5,384,805 - BWI76_RS26055 0.61 -2.9 5,384,904 + BWI76_RS26055 0.68 -3.9 5,385,042 + BWI76_RS26055 0.78 -3.6 5,385,042 + BWI76_RS26055 0.78 -2.3 5,385,042 + BWI76_RS26055 0.78 -2.3 5,385,050 - BWI76_RS26055 0.79 -4.9 5,385,195 - BWI76_RS26055 0.89 -2.4 5,385,216 - -3.9 5,385,230 + -1.7 5,385,238 - -1.3 5,385,238 - -2.3 5,385,304 - -1.8 5,385,353 + +1.1 5,385,427 + +0.5 5,385,428 - -0.5 5,385,440 - +0.8 5,385,542 - BWI76_RS26060 0.11 -0.2 5,385,542 - BWI76_RS26060 0.11 -1.1 5,385,564 + BWI76_RS26060 0.13 -0.0 5,385,610 + BWI76_RS26060 0.18 -0.2 5,385,706 - BWI76_RS26060 0.28 +0.2 5,385,867 - BWI76_RS26060 0.44 +0.2 5,386,074 - BWI76_RS26060 0.64 -0.7 5,386,074 - BWI76_RS26060 0.64 -1.6 5,386,074 - BWI76_RS26060 0.64 -0.4 5,386,190 + BWI76_RS26060 0.76 -0.4 5,386,236 - BWI76_RS26060 0.80 -1.2 5,386,258 + BWI76_RS26060 0.82 -0.1 5,386,266 - BWI76_RS26060 0.83 -1.0 5,386,266 - BWI76_RS26060 0.83 +1.3
Or see this region's nucleotide sequence