Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS22930 and BWI76_RS22935 are separated by 1 nucleotides BWI76_RS22935 and BWI76_RS22940 are separated by 57 nucleotides BWI76_RS22940 and BWI76_RS22945 are separated by 61 nucleotides
BWI76_RS22930: BWI76_RS22930 - L-fuculokinase (EC 2.7.1.51) (from data) , at 4,788,541 to 4,789,959
_RS22930
BWI76_RS22935: BWI76_RS22935 - L-fucose mutarotase, at 4,789,961 to 4,790,383
_RS22935
BWI76_RS22940: BWI76_RS22940 - DNA-binding transcriptional activator FucR, at 4,790,441 to 4,791,151
_RS22940
BWI76_RS22945: BWI76_RS22945 - 23S rRNA (cytidine(2498)-2'-O)-methyltransferase RlmM, at 4,791,213 to 4,792,313
_RS22945
Position (kb)
4789
4790
4791 Strain fitness (log2 ratio)
-2
-1
0
1 at 4789.289 kb on - strand, within BWI76_RS22930 at 4789.302 kb on + strand, within BWI76_RS22930 at 4789.490 kb on - strand, within BWI76_RS22930 at 4789.680 kb on + strand, within BWI76_RS22930 at 4789.944 kb on + strand at 4790.064 kb on - strand, within BWI76_RS22935 at 4790.311 kb on - strand, within BWI76_RS22935 at 4790.564 kb on + strand, within BWI76_RS22940 at 4790.920 kb on + strand, within BWI76_RS22940 at 4790.971 kb on - strand, within BWI76_RS22940 at 4791.258 kb on + strand at 4791.324 kb on - strand, within BWI76_RS22945
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 4,789,289 - BWI76_RS22930 0.53 +0.5 4,789,302 + BWI76_RS22930 0.54 +0.9 4,789,490 - BWI76_RS22930 0.67 +1.0 4,789,680 + BWI76_RS22930 0.80 -1.8 4,789,944 + +0.2 4,790,064 - BWI76_RS22935 0.24 -1.1 4,790,311 - BWI76_RS22935 0.83 -1.5 4,790,564 + BWI76_RS22940 0.17 -0.6 4,790,920 + BWI76_RS22940 0.67 +0.3 4,790,971 - BWI76_RS22940 0.75 -1.1 4,791,258 + +0.7 4,791,324 - BWI76_RS22945 0.10 -0.3
Or see this region's nucleotide sequence