Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10660 and BWI76_RS10665 are separated by 51 nucleotides BWI76_RS10665 and BWI76_RS10670 are separated by 20 nucleotides BWI76_RS10670 and BWI76_RS10675 are separated by 308 nucleotides
BWI76_RS10660: BWI76_RS10660 - formate C-acetyltransferase/glycerol dehydratase family glycyl radical enzyme, at 2,250,063 to 2,252,360
_RS10660
BWI76_RS10665: BWI76_RS10665 - PTS system fructose-like transporter subunit EIIB, at 2,252,412 to 2,252,732
_RS10665
BWI76_RS10670: BWI76_RS10670 - PTS fructose transporter subunit IIC, at 2,252,753 to 2,253,829
_RS10670
BWI76_RS10675: BWI76_RS10675 - phosphoenolpyruvate--protein phosphotransferase, at 2,254,138 to 2,256,639
_RS10675
Position (kb)
2252
2253
2254 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2251.766 kb on - strand, within BWI76_RS10660 at 2251.904 kb on - strand, within BWI76_RS10660 at 2251.904 kb on - strand, within BWI76_RS10660 at 2251.948 kb on + strand, within BWI76_RS10660 at 2252.107 kb on + strand, within BWI76_RS10660 at 2252.677 kb on + strand, within BWI76_RS10665 at 2252.682 kb on + strand, within BWI76_RS10665 at 2252.685 kb on - strand, within BWI76_RS10665 at 2252.790 kb on + strand at 2252.823 kb on + strand at 2252.841 kb on - strand at 2252.934 kb on + strand, within BWI76_RS10670 at 2252.934 kb on + strand, within BWI76_RS10670 at 2252.980 kb on - strand, within BWI76_RS10670 at 2253.276 kb on + strand, within BWI76_RS10670 at 2253.676 kb on + strand, within BWI76_RS10670 at 2253.783 kb on - strand at 2254.178 kb on + strand at 2254.365 kb on - strand at 2254.570 kb on - strand, within BWI76_RS10675 at 2254.815 kb on - strand, within BWI76_RS10675
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 2,251,766 - BWI76_RS10660 0.74 -1.3 2,251,904 - BWI76_RS10660 0.80 -1.5 2,251,904 - BWI76_RS10660 0.80 +0.3 2,251,948 + BWI76_RS10660 0.82 +1.1 2,252,107 + BWI76_RS10660 0.89 +0.4 2,252,677 + BWI76_RS10665 0.83 -1.6 2,252,682 + BWI76_RS10665 0.84 +0.4 2,252,685 - BWI76_RS10665 0.85 +0.7 2,252,790 + +1.1 2,252,823 + +0.3 2,252,841 - +0.4 2,252,934 + BWI76_RS10670 0.17 +0.7 2,252,934 + BWI76_RS10670 0.17 +2.1 2,252,980 - BWI76_RS10670 0.21 +0.4 2,253,276 + BWI76_RS10670 0.49 +0.8 2,253,676 + BWI76_RS10670 0.86 -2.9 2,253,783 - -0.4 2,254,178 + -0.0 2,254,365 - +0.7 2,254,570 - BWI76_RS10675 0.17 +0.0 2,254,815 - BWI76_RS10675 0.27 +0.0
Or see this region's nucleotide sequence