Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01105 and BWI76_RS01110 overlap by 23 nucleotides BWI76_RS01110 and BWI76_RS01115 are separated by 4 nucleotides BWI76_RS01115 and BWI76_RS01120 are separated by 1 nucleotides
BWI76_RS01105: BWI76_RS01105 - glucose-1-phosphate thymidylyltransferase, at 227,906 to 228,787
_RS01105
BWI76_RS01110: BWI76_RS01110 - TDP-fucosamine acetyltransferase, at 228,765 to 229,439
_RS01110
BWI76_RS01115: BWI76_RS01115 - dTDP-4-amino-4,6-dideoxygalactose transaminase, at 229,444 to 230,574
_RS01115
BWI76_RS01120: BWI76_RS01120 - O-antigen translocase, at 230,576 to 231,826
_RS01120
Position (kb) 229
230
231 Strain fitness (log2 ratio) -2
-1
0
1 at 228.654 kb on + strand, within BWI76_RS01105 at 228.789 kb on - strand at 228.839 kb on - strand, within BWI76_RS01110 at 228.927 kb on + strand, within BWI76_RS01110 at 229.276 kb on - strand, within BWI76_RS01110 at 229.279 kb on + strand, within BWI76_RS01110 at 229.315 kb on + strand, within BWI76_RS01110 at 229.536 kb on - strand at 229.602 kb on + strand, within BWI76_RS01115 at 229.711 kb on - strand, within BWI76_RS01115 at 229.716 kb on - strand, within BWI76_RS01115 at 230.473 kb on + strand at 230.481 kb on - strand at 230.528 kb on - strand at 230.592 kb on - strand at 230.926 kb on - strand, within BWI76_RS01120 at 230.953 kb on + strand, within BWI76_RS01120 at 230.953 kb on + strand, within BWI76_RS01120 at 231.208 kb on + strand, within BWI76_RS01120 at 231.388 kb on + strand, within BWI76_RS01120 at 231.410 kb on + strand, within BWI76_RS01120
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 228,654 + BWI76_RS01105 0.85 +0.0 228,789 - -0.6 228,839 - BWI76_RS01110 0.11 +0.3 228,927 + BWI76_RS01110 0.24 -1.8 229,276 - BWI76_RS01110 0.76 -1.1 229,279 + BWI76_RS01110 0.76 -0.1 229,315 + BWI76_RS01110 0.81 -0.7 229,536 - +0.6 229,602 + BWI76_RS01115 0.14 -1.4 229,711 - BWI76_RS01115 0.24 -0.4 229,716 - BWI76_RS01115 0.24 -1.3 230,473 + -0.7 230,481 - -1.1 230,528 - -0.8 230,592 - +0.8 230,926 - BWI76_RS01120 0.28 -0.2 230,953 + BWI76_RS01120 0.30 +0.8 230,953 + BWI76_RS01120 0.30 -0.3 231,208 + BWI76_RS01120 0.51 -0.1 231,388 + BWI76_RS01120 0.65 +0.5 231,410 + BWI76_RS01120 0.67 -1.1
Or see this region's nucleotide sequence