Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00385 and BWI76_RS00390 are separated by 8 nucleotides BWI76_RS00390 and BWI76_RS00395 overlap by 49 nucleotides BWI76_RS00395 and BWI76_RS00400 are separated by 57 nucleotides
BWI76_RS00385: BWI76_RS00385 - nitrogen assimilation regulatory protein, at 80,878 to 82,287
_RS00385
BWI76_RS00390: BWI76_RS00390 - two-component system sensor histidine kinase NtrB, at 82,296 to 83,345
_RS00390
BWI76_RS00395: BWI76_RS00395 - hypothetical protein, at 83,297 to 83,476
_RS00395
BWI76_RS00400: BWI76_RS00400 - glutamine synthetase, at 83,534 to 84,943
_RS00400
Position (kb) 82
83
84 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 81.432 kb on + strand, within BWI76_RS00385 at 81.432 kb on + strand, within BWI76_RS00385 at 81.600 kb on + strand, within BWI76_RS00385 at 81.652 kb on - strand, within BWI76_RS00385 at 81.934 kb on - strand, within BWI76_RS00385 at 82.010 kb on + strand, within BWI76_RS00385 at 82.010 kb on - strand, within BWI76_RS00385 at 82.201 kb on + strand at 82.201 kb on + strand at 82.209 kb on - strand at 82.209 kb on - strand at 82.255 kb on - strand at 82.334 kb on + strand at 82.334 kb on + strand at 82.400 kb on + strand at 82.493 kb on - strand, within BWI76_RS00390 at 82.861 kb on + strand, within BWI76_RS00390 at 82.889 kb on + strand, within BWI76_RS00390 at 83.033 kb on + strand, within BWI76_RS00390 at 83.040 kb on + strand, within BWI76_RS00390 at 83.142 kb on + strand, within BWI76_RS00390 at 83.186 kb on - strand, within BWI76_RS00390 at 83.302 kb on + strand at 83.501 kb on - strand at 83.508 kb on - strand at 83.541 kb on + strand at 83.636 kb on + strand at 83.695 kb on + strand, within BWI76_RS00400 at 83.819 kb on - strand, within BWI76_RS00400
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 81,432 + BWI76_RS00385 0.39 -1.8 81,432 + BWI76_RS00385 0.39 -2.2 81,600 + BWI76_RS00385 0.51 -3.1 81,652 - BWI76_RS00385 0.55 -1.3 81,934 - BWI76_RS00385 0.75 -3.4 82,010 + BWI76_RS00385 0.80 -1.6 82,010 - BWI76_RS00385 0.80 -2.0 82,201 + -2.1 82,201 + -0.2 82,209 - -1.7 82,209 - -2.0 82,255 - -2.9 82,334 + -0.9 82,334 + -1.3 82,400 + -2.5 82,493 - BWI76_RS00390 0.19 -0.3 82,861 + BWI76_RS00390 0.54 -2.9 82,889 + BWI76_RS00390 0.56 -0.4 83,033 + BWI76_RS00390 0.70 -2.5 83,040 + BWI76_RS00390 0.71 -1.7 83,142 + BWI76_RS00390 0.81 -1.9 83,186 - BWI76_RS00390 0.85 -4.6 83,302 + -4.5 83,501 - +0.2 83,508 - -1.2 83,541 + -2.4 83,636 + -0.3 83,695 + BWI76_RS00400 0.11 -0.1 83,819 - BWI76_RS00400 0.20 -1.3
Or see this region's nucleotide sequence