Experiment: L-Methionine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c04810 and PGA1_c04820 overlap by 4 nucleotides PGA1_c04820 and PGA1_c04830 are separated by 326 nucleotides PGA1_c04830 and PGA1_c04840 are separated by 344 nucleotides PGA1_c04840 and PGA1_c04850 overlap by 1 nucleotides PGA1_c04850 and PGA1_c04860 are separated by 7 nucleotides
PGA1_c04810: PGA1_c04810 - 3-phosphoshikimate 1-carboxyvinyltransferase AroA, at 472,867 to 474,216
_c04810
PGA1_c04820: PGA1_c04820 - cytidylate kinase Cmk, at 474,213 to 474,818
_c04820
PGA1_c04830: PGA1_c04830 - 30S ribosomal protein S1, at 475,145 to 476,824
_c04830
PGA1_c04840: PGA1_c04840 - integration host factor subunit beta, at 477,169 to 477,453
_c04840
PGA1_c04850: PGA1_c04850 - Protein of unknown function (DUF1049)., at 477,453 to 477,803
_c04850
PGA1_c04860: PGA1_c04860 - N-(5'-phosphoribosyl)anthranilate isomerase TrpF, at 477,811 to 478,464
_c04860
Position (kb) 475
476
477 Strain fitness (log2 ratio) -2
-1
0
1 at 474.825 kb on - strand at 474.865 kb on + strand at 474.875 kb on + strand at 474.926 kb on - strand at 476.899 kb on + strand at 476.992 kb on + strand at 477.039 kb on - strand at 477.070 kb on - strand at 477.075 kb on + strand at 477.080 kb on + strand at 477.083 kb on - strand at 477.083 kb on - strand at 477.145 kb on + strand at 477.475 kb on + strand at 477.545 kb on + strand, within PGA1_c04850 at 477.749 kb on + strand, within PGA1_c04850 at 477.767 kb on + strand, within PGA1_c04850 at 477.783 kb on + strand at 477.808 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Methionine (N) remove 474,825 - +0.4 474,865 + +0.4 474,875 + -2.5 474,926 - +0.1 476,899 + -0.3 476,992 + +0.4 477,039 - -0.5 477,070 - -0.6 477,075 + -0.2 477,080 + -1.1 477,083 - -0.7 477,083 - -0.5 477,145 + -1.0 477,475 + -1.1 477,545 + PGA1_c04850 0.26 -1.6 477,749 + PGA1_c04850 0.84 -0.2 477,767 + PGA1_c04850 0.89 +0.6 477,783 + -1.9 477,808 + -0.1
Or see this region's nucleotide sequence