Experiment: L-Methionine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_65p00230 and PGA1_65p00240 are separated by 531 nucleotides PGA1_65p00240 and PGA1_65p00250 are separated by 3 nucleotides PGA1_65p00250 and PGA1_65p00260 are separated by 129 nucleotides
PGA1_65p00230: PGA1_65p00230 - dTDP-4-dehydrorhamnose reductase RfbD, at 29,374 to 30,222
_65p00230
PGA1_65p00240: PGA1_65p00240 - dTDP-glucose 4,6-dehydratase RffG, at 30,754 to 31,806
_65p00240
PGA1_65p00250: PGA1_65p00250 - dTDP-4-dehydrorhamnose 3,5-epimerase RfbC, at 31,810 to 32,445
_65p00250
PGA1_65p00260: PGA1_65p00260 - putative metallopeptidase, at 32,575 to 34,026
_65p00260
Position (kb)
30
31
32 Strain fitness (log2 ratio)
-2
-1
0
1 at 29.931 kb on + strand, within PGA1_65p00230 at 29.991 kb on + strand, within PGA1_65p00230 at 30.260 kb on - strand at 30.261 kb on - strand at 30.294 kb on - strand at 30.354 kb on - strand at 30.537 kb on - strand at 30.631 kb on + strand at 30.847 kb on + strand at 30.855 kb on - strand at 31.249 kb on - strand, within PGA1_65p00240 at 31.274 kb on - strand, within PGA1_65p00240 at 31.286 kb on - strand, within PGA1_65p00240 at 31.298 kb on - strand, within PGA1_65p00240 at 31.450 kb on - strand, within PGA1_65p00240 at 31.652 kb on - strand, within PGA1_65p00240 at 31.698 kb on + strand, within PGA1_65p00240 at 31.969 kb on + strand, within PGA1_65p00250 at 32.372 kb on + strand, within PGA1_65p00250 at 32.380 kb on - strand, within PGA1_65p00250 at 32.393 kb on + strand at 32.456 kb on + strand at 32.464 kb on - strand at 32.469 kb on - strand at 32.537 kb on + strand at 32.574 kb on + strand at 32.632 kb on - strand at 32.805 kb on - strand, within PGA1_65p00260
Per-strain Table
Position Strand Gene LocusTag Fraction L-Methionine (N) remove 29,931 + PGA1_65p00230 0.66 -1.1 29,991 + PGA1_65p00230 0.73 -0.9 30,260 - -0.6 30,261 - -1.3 30,294 - -0.3 30,354 - +1.7 30,537 - +0.3 30,631 + +0.4 30,847 + +0.0 30,855 - -0.5 31,249 - PGA1_65p00240 0.47 -0.2 31,274 - PGA1_65p00240 0.49 -0.3 31,286 - PGA1_65p00240 0.51 +0.7 31,298 - PGA1_65p00240 0.52 -1.3 31,450 - PGA1_65p00240 0.66 -2.3 31,652 - PGA1_65p00240 0.85 -1.6 31,698 + PGA1_65p00240 0.90 -2.3 31,969 + PGA1_65p00250 0.25 -1.7 32,372 + PGA1_65p00250 0.88 +0.0 32,380 - PGA1_65p00250 0.90 -1.3 32,393 + +1.3 32,456 + -1.1 32,464 - +0.7 32,469 - +1.1 32,537 + -0.6 32,574 + -0.0 32,632 - -0.0 32,805 - PGA1_65p00260 0.16 -1.0
Or see this region's nucleotide sequence