Experiment: L-Methionine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_65p00200 and PGA1_65p00210 are separated by 317 nucleotides PGA1_65p00210 and PGA1_65p00220 are separated by 0 nucleotides PGA1_65p00220 and PGA1_65p00230 overlap by 4 nucleotides
PGA1_65p00200: PGA1_65p00200 - putative polysaccharide biosynthesis/export protein, at 26,175 to 27,314
_65p00200
PGA1_65p00210: PGA1_65p00210 - hypothetical protein, at 27,632 to 28,486
_65p00210
PGA1_65p00220: PGA1_65p00220 - glucose-1-phosphate thymidylyltransferase RfbA, at 28,487 to 29,377
_65p00220
PGA1_65p00230: PGA1_65p00230 - dTDP-4-dehydrorhamnose reductase RfbD, at 29,374 to 30,222
_65p00230
Position (kb) 27
28
29 Strain fitness (log2 ratio) -1
0
1
2
3 at 27.000 kb on + strand, within PGA1_65p00200 at 27.308 kb on + strand at 27.391 kb on + strand at 27.523 kb on + strand at 27.620 kb on - strand at 27.725 kb on + strand, within PGA1_65p00210 at 27.826 kb on - strand, within PGA1_65p00210 at 27.914 kb on - strand, within PGA1_65p00210 at 28.046 kb on + strand, within PGA1_65p00210 at 28.123 kb on + strand, within PGA1_65p00210 at 28.292 kb on - strand, within PGA1_65p00210 at 28.363 kb on - strand, within PGA1_65p00210 at 28.416 kb on + strand at 29.387 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Methionine (N) remove 27,000 + PGA1_65p00200 0.72 -0.2 27,308 + +0.4 27,391 + +1.5 27,523 + +0.7 27,620 - -0.7 27,725 + PGA1_65p00210 0.11 +1.0 27,826 - PGA1_65p00210 0.23 +0.1 27,914 - PGA1_65p00210 0.33 +1.2 28,046 + PGA1_65p00210 0.48 -0.2 28,123 + PGA1_65p00210 0.57 -0.0 28,292 - PGA1_65p00210 0.77 +0.9 28,363 - PGA1_65p00210 0.85 +3.1 28,416 + +0.1 29,387 + -1.0
Or see this region's nucleotide sequence