Experiment: L-Methionine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c14510 and PGA1_c14520 are separated by 5 nucleotides PGA1_c14520 and PGA1_c14530 are separated by 114 nucleotides PGA1_c14530 and PGA1_c14540 overlap by 8 nucleotides PGA1_c14540 and PGA1_c14550 are separated by 61 nucleotides
PGA1_c14510: PGA1_c14510 - 50S ribosomal protein L13, at 1,506,463 to 1,506,921
_c14510
PGA1_c14520: PGA1_c14520 - 30S ribosomal protein S9, at 1,506,927 to 1,507,412
_c14520
PGA1_c14530: PGA1_c14530 - transcriptional regulator, TetR family, at 1,507,527 to 1,508,144
_c14530
PGA1_c14540: PGA1_c14540 - putative polyphosphate kinase 2, at 1,508,137 to 1,509,006
_c14540
PGA1_c14550: PGA1_c14550 - Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily), at 1,509,068 to 1,510,060
_c14550
Position (kb)
1507
1508
1509 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1507.480 kb on + strand at 1507.515 kb on - strand at 1507.515 kb on - strand at 1507.547 kb on + strand at 1507.555 kb on - strand at 1507.853 kb on + strand, within PGA1_c14530 at 1507.877 kb on - strand, within PGA1_c14530 at 1507.892 kb on + strand, within PGA1_c14530 at 1508.100 kb on - strand at 1508.100 kb on - strand at 1508.355 kb on + strand, within PGA1_c14540 at 1508.480 kb on - strand, within PGA1_c14540 at 1508.480 kb on - strand, within PGA1_c14540 at 1508.499 kb on + strand, within PGA1_c14540 at 1508.507 kb on + strand, within PGA1_c14540 at 1508.507 kb on - strand, within PGA1_c14540 at 1508.507 kb on - strand, within PGA1_c14540 at 1508.515 kb on - strand, within PGA1_c14540 at 1508.678 kb on + strand, within PGA1_c14540 at 1508.678 kb on + strand, within PGA1_c14540 at 1508.696 kb on - strand, within PGA1_c14540 at 1509.087 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Methionine (N) remove 1,507,480 + -0.2 1,507,515 - +0.2 1,507,515 - -0.0 1,507,547 + -0.6 1,507,555 - +1.0 1,507,853 + PGA1_c14530 0.53 -1.2 1,507,877 - PGA1_c14530 0.57 +1.0 1,507,892 + PGA1_c14530 0.59 +1.0 1,508,100 - +0.1 1,508,100 - -0.9 1,508,355 + PGA1_c14540 0.25 -0.8 1,508,480 - PGA1_c14540 0.39 -0.3 1,508,480 - PGA1_c14540 0.39 +1.0 1,508,499 + PGA1_c14540 0.42 -0.9 1,508,507 + PGA1_c14540 0.43 +0.3 1,508,507 - PGA1_c14540 0.43 -0.8 1,508,507 - PGA1_c14540 0.43 -1.9 1,508,515 - PGA1_c14540 0.43 +0.7 1,508,678 + PGA1_c14540 0.62 -0.2 1,508,678 + PGA1_c14540 0.62 +1.9 1,508,696 - PGA1_c14540 0.64 -0.3 1,509,087 + -1.0
Or see this region's nucleotide sequence