Experiment: L-Methionine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c11750 and PGA1_c11760 are separated by 135 nucleotides PGA1_c11760 and PGA1_c11770 are separated by 69 nucleotides PGA1_c11770 and PGA1_c11780 are separated by 109 nucleotides
PGA1_c11750: PGA1_c11750 - L-glutamate gamma-semialdehyde dehydrogenase (EC 1.2.1.88); Proline dehydrogenase (EC 1.5.5.2) (from data) , at 1,215,988 to 1,219,464
_c11750
PGA1_c11760: PGA1_c11760 - putative proline dehydrogenase transcriptional activator, at 1,219,600 to 1,220,073
_c11760
PGA1_c11770: PGA1_c11770 - tetratricopeptide repeat-containing protein, at 1,220,143 to 1,220,775
_c11770
PGA1_c11780: PGA1_c11780 - tRNA-Leu, at 1,220,885 to 1,220,970
_c11780
Position (kb)
1219
1220
1221 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1218.669 kb on + strand, within PGA1_c11750 at 1218.736 kb on + strand, within PGA1_c11750 at 1218.736 kb on + strand, within PGA1_c11750 at 1218.945 kb on - strand, within PGA1_c11750 at 1218.945 kb on - strand, within PGA1_c11750 at 1218.985 kb on + strand, within PGA1_c11750 at 1218.985 kb on + strand, within PGA1_c11750 at 1218.990 kb on + strand, within PGA1_c11750 at 1219.055 kb on + strand, within PGA1_c11750 at 1219.055 kb on + strand, within PGA1_c11750 at 1219.063 kb on - strand, within PGA1_c11750 at 1219.073 kb on - strand, within PGA1_c11750 at 1219.076 kb on + strand, within PGA1_c11750 at 1219.175 kb on + strand at 1219.180 kb on + strand at 1219.180 kb on + strand at 1219.180 kb on + strand at 1219.239 kb on + strand at 1219.395 kb on + strand at 1219.402 kb on + strand at 1219.651 kb on + strand, within PGA1_c11760 at 1219.651 kb on + strand, within PGA1_c11760 at 1219.665 kb on - strand, within PGA1_c11760 at 1219.687 kb on - strand, within PGA1_c11760 at 1219.748 kb on - strand, within PGA1_c11760 at 1219.803 kb on + strand, within PGA1_c11760 at 1219.897 kb on + strand, within PGA1_c11760 at 1219.897 kb on + strand, within PGA1_c11760 at 1219.989 kb on + strand, within PGA1_c11760 at 1220.017 kb on + strand, within PGA1_c11760 at 1220.025 kb on - strand, within PGA1_c11760 at 1220.060 kb on - strand at 1220.070 kb on - strand at 1220.133 kb on + strand at 1220.133 kb on + strand at 1220.151 kb on + strand at 1220.151 kb on + strand at 1220.151 kb on + strand at 1220.151 kb on + strand at 1220.159 kb on - strand at 1220.159 kb on - strand at 1220.210 kb on - strand, within PGA1_c11770 at 1220.224 kb on - strand, within PGA1_c11770 at 1220.230 kb on + strand, within PGA1_c11770 at 1220.290 kb on - strand, within PGA1_c11770 at 1220.290 kb on - strand, within PGA1_c11770 at 1220.305 kb on + strand, within PGA1_c11770 at 1220.466 kb on + strand, within PGA1_c11770 at 1220.548 kb on + strand, within PGA1_c11770 at 1220.556 kb on - strand, within PGA1_c11770 at 1220.556 kb on - strand, within PGA1_c11770 at 1220.556 kb on - strand, within PGA1_c11770 at 1220.556 kb on - strand, within PGA1_c11770 at 1220.556 kb on - strand, within PGA1_c11770 at 1220.570 kb on + strand, within PGA1_c11770 at 1220.580 kb on - strand, within PGA1_c11770 at 1220.599 kb on + strand, within PGA1_c11770 at 1220.599 kb on + strand, within PGA1_c11770 at 1220.665 kb on + strand, within PGA1_c11770 at 1220.711 kb on - strand, within PGA1_c11770 at 1220.769 kb on + strand at 1220.777 kb on + strand at 1220.870 kb on + strand at 1220.870 kb on + strand at 1221.030 kb on + strand at 1221.070 kb on + strand at 1221.073 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Methionine (N) remove 1,218,669 + PGA1_c11750 0.77 -0.3 1,218,736 + PGA1_c11750 0.79 +0.1 1,218,736 + PGA1_c11750 0.79 -0.3 1,218,945 - PGA1_c11750 0.85 -0.2 1,218,945 - PGA1_c11750 0.85 -0.2 1,218,985 + PGA1_c11750 0.86 +0.6 1,218,985 + PGA1_c11750 0.86 -0.2 1,218,990 + PGA1_c11750 0.86 -0.2 1,219,055 + PGA1_c11750 0.88 +1.2 1,219,055 + PGA1_c11750 0.88 +0.2 1,219,063 - PGA1_c11750 0.88 +0.2 1,219,073 - PGA1_c11750 0.89 -0.3 1,219,076 + PGA1_c11750 0.89 -1.4 1,219,175 + -0.9 1,219,180 + +0.0 1,219,180 + -0.1 1,219,180 + +0.5 1,219,239 + -0.6 1,219,395 + +0.1 1,219,402 + -0.1 1,219,651 + PGA1_c11760 0.11 +0.4 1,219,651 + PGA1_c11760 0.11 +0.0 1,219,665 - PGA1_c11760 0.14 -0.8 1,219,687 - PGA1_c11760 0.18 +0.5 1,219,748 - PGA1_c11760 0.31 +0.2 1,219,803 + PGA1_c11760 0.43 +0.2 1,219,897 + PGA1_c11760 0.63 +0.1 1,219,897 + PGA1_c11760 0.63 +1.0 1,219,989 + PGA1_c11760 0.82 -0.3 1,220,017 + PGA1_c11760 0.88 +0.8 1,220,025 - PGA1_c11760 0.90 -0.2 1,220,060 - +0.0 1,220,070 - -0.1 1,220,133 + +0.4 1,220,133 + +1.0 1,220,151 + +0.5 1,220,151 + +0.1 1,220,151 + +0.7 1,220,151 + +0.2 1,220,159 - +0.1 1,220,159 - -0.5 1,220,210 - PGA1_c11770 0.11 +0.8 1,220,224 - PGA1_c11770 0.13 -0.3 1,220,230 + PGA1_c11770 0.14 -0.3 1,220,290 - PGA1_c11770 0.23 +0.4 1,220,290 - PGA1_c11770 0.23 +0.3 1,220,305 + PGA1_c11770 0.26 +0.4 1,220,466 + PGA1_c11770 0.51 +0.1 1,220,548 + PGA1_c11770 0.64 +0.8 1,220,556 - PGA1_c11770 0.65 -0.1 1,220,556 - PGA1_c11770 0.65 -0.1 1,220,556 - PGA1_c11770 0.65 -0.8 1,220,556 - PGA1_c11770 0.65 +0.7 1,220,556 - PGA1_c11770 0.65 -0.5 1,220,570 + PGA1_c11770 0.67 -2.2 1,220,580 - PGA1_c11770 0.69 -1.2 1,220,599 + PGA1_c11770 0.72 +0.1 1,220,599 + PGA1_c11770 0.72 -0.4 1,220,665 + PGA1_c11770 0.82 +0.4 1,220,711 - PGA1_c11770 0.90 +0.3 1,220,769 + -0.4 1,220,777 + +0.5 1,220,870 + -2.8 1,220,870 + -2.4 1,221,030 + +0.4 1,221,070 + -0.9 1,221,073 - -0.2
Or see this region's nucleotide sequence