Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20790 and BWI76_RS20795 are separated by 11 nucleotides BWI76_RS20795 and BWI76_RS20800 are separated by 114 nucleotides BWI76_RS20800 and BWI76_RS20805 are separated by 36 nucleotides
BWI76_RS20790: BWI76_RS20790 - aldehyde dehydrogenase, at 4,345,900 to 4,347,303
_RS20790
BWI76_RS20795: BWI76_RS20795 - ethanolamine catabolic microcompartment shell protein EutN, at 4,347,315 to 4,347,602
_RS20795
BWI76_RS20800: BWI76_RS20800 - carboxysome shell protein, at 4,347,717 to 4,348,007
_RS20800
BWI76_RS20805: BWI76_RS20805 - phosphate acetyltransferase, at 4,348,044 to 4,349,060
_RS20805
Position (kb) 4347
4348 Strain fitness (log2 ratio) -1
0
1 at 4346.342 kb on + strand, within BWI76_RS20790 at 4346.544 kb on - strand, within BWI76_RS20790 at 4346.605 kb on + strand, within BWI76_RS20790 at 4346.644 kb on - strand, within BWI76_RS20790 at 4347.523 kb on + strand, within BWI76_RS20795 at 4347.955 kb on + strand, within BWI76_RS20800 at 4347.963 kb on - strand, within BWI76_RS20800 at 4347.963 kb on - strand, within BWI76_RS20800 at 4348.572 kb on + strand, within BWI76_RS20805 at 4348.572 kb on + strand, within BWI76_RS20805 at 4348.572 kb on + strand, within BWI76_RS20805
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 4,346,342 + BWI76_RS20790 0.31 -1.0 4,346,544 - BWI76_RS20790 0.46 +0.8 4,346,605 + BWI76_RS20790 0.50 +0.4 4,346,644 - BWI76_RS20790 0.53 +0.1 4,347,523 + BWI76_RS20795 0.72 -1.7 4,347,955 + BWI76_RS20800 0.82 -0.3 4,347,963 - BWI76_RS20800 0.85 -0.6 4,347,963 - BWI76_RS20800 0.85 -0.4 4,348,572 + BWI76_RS20805 0.52 +0.2 4,348,572 + BWI76_RS20805 0.52 -0.4 4,348,572 + BWI76_RS20805 0.52 +0.5
Or see this region's nucleotide sequence