Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01020 and BWI76_RS01025 are separated by 3 nucleotides BWI76_RS01025 and BWI76_RS01030 overlap by 17 nucleotides BWI76_RS01030 and BWI76_RS01035 are separated by 18 nucleotides
BWI76_RS01020: BWI76_RS01020 - DNA helicase Rep, at 207,493 to 209,511
_RS01020
BWI76_RS01025: BWI76_RS01025 - hypothetical protein, at 209,515 to 210,477
_RS01025
BWI76_RS01030: BWI76_RS01030 - cytosine/purine, uracil, thiamine, allantoin permease family protein, at 210,461 to 211,876
_RS01030
BWI76_RS01035: BWI76_RS01035 - ADP-ribosylation/crystallin J1, at 211,895 to 212,911
_RS01035
Position (kb) 210
211
212 Strain fitness (log2 ratio) -2
-1
0
1
2 at 209.478 kb on + strand at 209.635 kb on + strand, within BWI76_RS01025 at 209.705 kb on + strand, within BWI76_RS01025 at 209.713 kb on - strand, within BWI76_RS01025 at 209.829 kb on - strand, within BWI76_RS01025 at 209.848 kb on + strand, within BWI76_RS01025 at 209.865 kb on - strand, within BWI76_RS01025 at 209.865 kb on - strand, within BWI76_RS01025 at 209.865 kb on - strand, within BWI76_RS01025 at 210.021 kb on + strand, within BWI76_RS01025 at 210.031 kb on + strand, within BWI76_RS01025 at 210.034 kb on - strand, within BWI76_RS01025 at 210.065 kb on + strand, within BWI76_RS01025 at 210.128 kb on - strand, within BWI76_RS01025 at 210.128 kb on - strand, within BWI76_RS01025 at 210.462 kb on - strand at 210.549 kb on + strand at 210.649 kb on + strand, within BWI76_RS01030 at 210.652 kb on - strand, within BWI76_RS01030 at 210.662 kb on - strand, within BWI76_RS01030 at 210.677 kb on + strand, within BWI76_RS01030 at 210.757 kb on + strand, within BWI76_RS01030 at 211.104 kb on + strand, within BWI76_RS01030 at 211.256 kb on - strand, within BWI76_RS01030 at 211.441 kb on + strand, within BWI76_RS01030 at 211.482 kb on - strand, within BWI76_RS01030 at 211.626 kb on + strand, within BWI76_RS01030 at 211.856 kb on + strand at 211.886 kb on - strand at 211.990 kb on + strand at 212.493 kb on + strand, within BWI76_RS01035 at 212.568 kb on - strand, within BWI76_RS01035 at 212.690 kb on + strand, within BWI76_RS01035 at 212.699 kb on + strand, within BWI76_RS01035 at 212.703 kb on - strand, within BWI76_RS01035 at 212.707 kb on - strand, within BWI76_RS01035 at 212.815 kb on + strand at 212.815 kb on + strand at 212.823 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 209,478 + +0.1 209,635 + BWI76_RS01025 0.12 -1.1 209,705 + BWI76_RS01025 0.20 -1.1 209,713 - BWI76_RS01025 0.21 +0.3 209,829 - BWI76_RS01025 0.33 -1.0 209,848 + BWI76_RS01025 0.35 +0.4 209,865 - BWI76_RS01025 0.36 +1.7 209,865 - BWI76_RS01025 0.36 -1.5 209,865 - BWI76_RS01025 0.36 +0.1 210,021 + BWI76_RS01025 0.53 -0.2 210,031 + BWI76_RS01025 0.54 -1.8 210,034 - BWI76_RS01025 0.54 +0.6 210,065 + BWI76_RS01025 0.57 +0.7 210,128 - BWI76_RS01025 0.64 +0.1 210,128 - BWI76_RS01025 0.64 -0.5 210,462 - +1.0 210,549 + -0.9 210,649 + BWI76_RS01030 0.13 -1.2 210,652 - BWI76_RS01030 0.13 +1.0 210,662 - BWI76_RS01030 0.14 +0.1 210,677 + BWI76_RS01030 0.15 +1.1 210,757 + BWI76_RS01030 0.21 +1.5 211,104 + BWI76_RS01030 0.45 +2.1 211,256 - BWI76_RS01030 0.56 -2.1 211,441 + BWI76_RS01030 0.69 +0.5 211,482 - BWI76_RS01030 0.72 +2.6 211,626 + BWI76_RS01030 0.82 +0.6 211,856 + +0.4 211,886 - +0.5 211,990 + +0.4 212,493 + BWI76_RS01035 0.59 +1.3 212,568 - BWI76_RS01035 0.66 +1.5 212,690 + BWI76_RS01035 0.78 -1.1 212,699 + BWI76_RS01035 0.79 -1.7 212,703 - BWI76_RS01035 0.79 -0.3 212,707 - BWI76_RS01035 0.80 -0.8 212,815 + +0.6 212,815 + +0.7 212,823 + -0.3
Or see this region's nucleotide sequence