Experiment: MoLS4 with Putrescine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt uraA and DVU1027 are separated by 233 nucleotides DVU1027 and cmk are separated by 236 nucleotides cmk and hisC overlap by 8 nucleotides
DVU1026: uraA - uracil permease (TIGR), at 1,130,018 to 1,131,277
uraA
DVU1027: DVU1027 - hypothetical protein (TIGR), at 1,131,511 to 1,132,482
DVU1027
DVU1028: cmk - cytidylate kinase (TIGR), at 1,132,719 to 1,133,417
cmk
DVU1029: hisC - histidinol-phosphate aminotransferase (EC 2.6.1.9) (from data) , at 1,133,410 to 1,134,531
hisC
Position (kb)
1131
1132
1133 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1130.650 kb on + strand, within uraA at 1130.658 kb on - strand, within uraA at 1130.658 kb on - strand, within uraA at 1130.820 kb on + strand, within uraA at 1130.839 kb on + strand, within uraA at 1130.865 kb on - strand, within uraA at 1130.886 kb on - strand, within uraA at 1130.907 kb on + strand, within uraA at 1130.928 kb on - strand, within uraA at 1130.959 kb on + strand, within uraA at 1131.044 kb on - strand, within uraA at 1131.106 kb on + strand, within uraA at 1131.106 kb on - strand, within uraA at 1131.106 kb on - strand, within uraA at 1131.404 kb on + strand at 1131.412 kb on - strand at 1131.421 kb on + strand at 1131.581 kb on + strand at 1131.581 kb on + strand at 1131.583 kb on - strand at 1131.681 kb on - strand, within DVU1027 at 1131.795 kb on + strand, within DVU1027 at 1131.808 kb on + strand, within DVU1027 at 1131.810 kb on + strand, within DVU1027 at 1131.816 kb on - strand, within DVU1027 at 1131.823 kb on + strand, within DVU1027 at 1131.838 kb on - strand, within DVU1027 at 1131.853 kb on - strand, within DVU1027 at 1131.922 kb on - strand, within DVU1027 at 1131.955 kb on + strand, within DVU1027 at 1131.957 kb on + strand, within DVU1027 at 1131.965 kb on - strand, within DVU1027 at 1131.973 kb on + strand, within DVU1027 at 1131.981 kb on - strand, within DVU1027 at 1132.001 kb on + strand, within DVU1027 at 1132.009 kb on - strand, within DVU1027 at 1132.009 kb on - strand, within DVU1027 at 1132.014 kb on - strand, within DVU1027 at 1132.072 kb on - strand, within DVU1027 at 1132.100 kb on + strand, within DVU1027 at 1132.124 kb on + strand, within DVU1027 at 1132.132 kb on + strand, within DVU1027 at 1132.271 kb on + strand, within DVU1027 at 1132.338 kb on - strand, within DVU1027 at 1132.354 kb on - strand, within DVU1027 at 1132.531 kb on + strand at 1132.679 kb on - strand at 1132.716 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with Putrescine (N) remove 1,130,650 + uraA DVU1026 0.50 -0.2 1,130,658 - uraA DVU1026 0.51 -0.3 1,130,658 - uraA DVU1026 0.51 +0.0 1,130,820 + uraA DVU1026 0.64 +0.4 1,130,839 + uraA DVU1026 0.65 -0.3 1,130,865 - uraA DVU1026 0.67 +0.0 1,130,886 - uraA DVU1026 0.69 +0.0 1,130,907 + uraA DVU1026 0.71 +0.1 1,130,928 - uraA DVU1026 0.72 -0.1 1,130,959 + uraA DVU1026 0.75 +0.5 1,131,044 - uraA DVU1026 0.81 +1.7 1,131,106 + uraA DVU1026 0.86 -0.6 1,131,106 - uraA DVU1026 0.86 -3.8 1,131,106 - uraA DVU1026 0.86 +0.1 1,131,404 + +0.2 1,131,412 - +0.7 1,131,421 + -0.2 1,131,581 + -0.7 1,131,581 + +1.4 1,131,583 - +0.4 1,131,681 - DVU1027 0.17 +0.4 1,131,795 + DVU1027 0.29 +0.4 1,131,808 + DVU1027 0.31 +0.3 1,131,810 + DVU1027 0.31 +0.4 1,131,816 - DVU1027 0.31 +0.6 1,131,823 + DVU1027 0.32 -0.1 1,131,838 - DVU1027 0.34 +0.0 1,131,853 - DVU1027 0.35 -0.6 1,131,922 - DVU1027 0.42 +1.0 1,131,955 + DVU1027 0.46 -0.4 1,131,957 + DVU1027 0.46 +0.5 1,131,965 - DVU1027 0.47 +0.6 1,131,973 + DVU1027 0.48 +0.4 1,131,981 - DVU1027 0.48 +0.3 1,132,001 + DVU1027 0.50 +0.9 1,132,009 - DVU1027 0.51 +0.5 1,132,009 - DVU1027 0.51 -1.1 1,132,014 - DVU1027 0.52 +0.7 1,132,072 - DVU1027 0.58 +0.5 1,132,100 + DVU1027 0.61 -0.0 1,132,124 + DVU1027 0.63 +0.4 1,132,132 + DVU1027 0.64 +0.8 1,132,271 + DVU1027 0.78 +0.6 1,132,338 - DVU1027 0.85 +0.3 1,132,354 - DVU1027 0.87 -0.3 1,132,531 + +0.6 1,132,679 - +0.0 1,132,716 + +0.8
Or see this region's nucleotide sequence