Experiment: LB - with RNA protect
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO4288 and pstB-2 are separated by 252 nucleotides pstB-2 and pstA are separated by 42 nucleotides pstA and pstC are separated by 29 nucleotides
SO4288: SO4288 - exopolysaccharide synthesis protein, putative (NCBI ptt file), at 4,467,552 to 4,468,184
SO4288
SO4289: pstB-2 - phosphate ABC transporter, ATP-binding protein (NCBI ptt file), at 4,468,437 to 4,469,186
pstB-2
SO4290: pstA - phosphate ABC transporter, permease protein (NCBI ptt file), at 4,469,229 to 4,470,065
pstA
SO4291: pstC - phosphate ABC transporter, permease protein (NCBI ptt file), at 4,470,095 to 4,470,967
pstC
Position (kb)
4468
4469
4470 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4467.555 kb on + strand at 4467.573 kb on + strand at 4467.591 kb on - strand at 4467.593 kb on + strand at 4467.602 kb on - strand at 4467.602 kb on - strand at 4467.612 kb on + strand at 4467.620 kb on - strand, within SO4288 at 4467.626 kb on - strand, within SO4288 at 4467.631 kb on - strand, within SO4288 at 4467.631 kb on - strand, within SO4288 at 4467.640 kb on + strand, within SO4288 at 4467.682 kb on - strand, within SO4288 at 4467.702 kb on + strand, within SO4288 at 4467.709 kb on + strand, within SO4288 at 4467.739 kb on - strand, within SO4288 at 4467.752 kb on - strand, within SO4288 at 4467.752 kb on - strand, within SO4288 at 4467.784 kb on + strand, within SO4288 at 4467.794 kb on + strand, within SO4288 at 4467.807 kb on - strand, within SO4288 at 4467.807 kb on - strand, within SO4288 at 4467.840 kb on - strand, within SO4288 at 4467.855 kb on + strand, within SO4288 at 4467.855 kb on + strand, within SO4288 at 4467.863 kb on - strand, within SO4288 at 4467.863 kb on - strand, within SO4288 at 4467.863 kb on - strand, within SO4288 at 4467.863 kb on - strand, within SO4288 at 4467.893 kb on + strand, within SO4288 at 4467.914 kb on + strand, within SO4288 at 4467.914 kb on + strand, within SO4288 at 4467.914 kb on + strand, within SO4288 at 4467.914 kb on + strand, within SO4288 at 4467.914 kb on + strand, within SO4288 at 4467.914 kb on + strand, within SO4288 at 4467.922 kb on - strand, within SO4288 at 4467.929 kb on - strand, within SO4288 at 4467.945 kb on + strand, within SO4288 at 4467.948 kb on - strand at 4467.992 kb on - strand, within SO4288 at 4468.000 kb on - strand, within SO4288 at 4468.000 kb on - strand, within SO4288 at 4468.000 kb on - strand, within SO4288 at 4468.000 kb on - strand, within SO4288 at 4468.081 kb on - strand, within SO4288 at 4468.083 kb on + strand, within SO4288 at 4468.101 kb on + strand, within SO4288 at 4468.109 kb on - strand, within SO4288 at 4468.134 kb on + strand at 4468.151 kb on + strand at 4468.159 kb on - strand at 4468.165 kb on + strand at 4468.186 kb on + strand at 4468.194 kb on - strand at 4468.270 kb on - strand at 4468.353 kb on + strand at 4468.364 kb on + strand at 4468.374 kb on + strand at 4468.476 kb on - strand at 4468.494 kb on + strand at 4468.512 kb on - strand, within pstB-2 at 4468.530 kb on - strand, within pstB-2 at 4468.655 kb on + strand, within pstB-2 at 4468.655 kb on + strand, within pstB-2 at 4468.663 kb on - strand, within pstB-2 at 4468.663 kb on - strand, within pstB-2 at 4468.663 kb on - strand, within pstB-2 at 4468.676 kb on - strand, within pstB-2 at 4468.752 kb on + strand, within pstB-2 at 4468.757 kb on + strand, within pstB-2 at 4468.775 kb on - strand, within pstB-2 at 4468.777 kb on + strand, within pstB-2 at 4468.785 kb on - strand, within pstB-2 at 4468.785 kb on - strand, within pstB-2 at 4468.796 kb on + strand, within pstB-2 at 4468.796 kb on + strand, within pstB-2 at 4468.796 kb on + strand, within pstB-2 at 4468.799 kb on - strand, within pstB-2 at 4468.982 kb on - strand, within pstB-2 at 4469.049 kb on + strand, within pstB-2 at 4469.057 kb on - strand, within pstB-2 at 4469.064 kb on + strand, within pstB-2 at 4469.082 kb on + strand, within pstB-2 at 4469.082 kb on + strand, within pstB-2 at 4469.082 kb on + strand, within pstB-2 at 4469.082 kb on + strand, within pstB-2 at 4469.090 kb on - strand, within pstB-2 at 4469.090 kb on - strand, within pstB-2 at 4469.090 kb on - strand, within pstB-2 at 4469.090 kb on - strand, within pstB-2 at 4469.090 kb on - strand, within pstB-2 at 4469.090 kb on - strand, within pstB-2 at 4469.090 kb on - strand, within pstB-2 at 4469.090 kb on - strand, within pstB-2 at 4469.090 kb on - strand, within pstB-2 at 4469.090 kb on - strand, within pstB-2 at 4469.090 kb on - strand, within pstB-2 at 4469.090 kb on - strand, within pstB-2 at 4469.275 kb on - strand at 4469.276 kb on - strand at 4469.290 kb on + strand at 4469.297 kb on - strand at 4469.308 kb on + strand at 4469.316 kb on - strand, within pstA at 4469.316 kb on - strand, within pstA at 4469.322 kb on - strand, within pstA at 4469.371 kb on + strand, within pstA at 4469.388 kb on + strand, within pstA at 4469.388 kb on + strand, within pstA at 4469.388 kb on + strand, within pstA at 4469.388 kb on + strand, within pstA at 4469.388 kb on + strand, within pstA at 4469.396 kb on - strand, within pstA at 4469.396 kb on - strand, within pstA at 4469.396 kb on - strand, within pstA at 4469.396 kb on - strand, within pstA at 4469.417 kb on + strand, within pstA at 4469.417 kb on + strand, within pstA at 4469.430 kb on + strand, within pstA at 4469.458 kb on - strand, within pstA at 4469.462 kb on + strand, within pstA at 4469.463 kb on + strand, within pstA at 4469.471 kb on - strand, within pstA at 4469.496 kb on + strand, within pstA at 4469.504 kb on - strand, within pstA at 4469.555 kb on - strand, within pstA at 4469.559 kb on + strand, within pstA at 4469.564 kb on + strand, within pstA at 4469.574 kb on + strand, within pstA at 4469.582 kb on + strand, within pstA at 4469.582 kb on - strand, within pstA at 4469.582 kb on - strand, within pstA at 4469.589 kb on + strand, within pstA at 4469.589 kb on + strand, within pstA at 4469.589 kb on + strand, within pstA at 4469.589 kb on + strand, within pstA at 4469.589 kb on + strand, within pstA at 4469.589 kb on + strand, within pstA at 4469.589 kb on + strand, within pstA at 4469.589 kb on + strand, within pstA at 4469.589 kb on + strand, within pstA at 4469.595 kb on + strand, within pstA at 4469.597 kb on - strand, within pstA at 4469.597 kb on - strand, within pstA at 4469.597 kb on - strand, within pstA at 4469.597 kb on - strand, within pstA at 4469.597 kb on - strand, within pstA at 4469.597 kb on - strand, within pstA at 4469.600 kb on + strand, within pstA at 4469.608 kb on - strand, within pstA at 4469.608 kb on - strand, within pstA at 4469.669 kb on + strand, within pstA at 4469.669 kb on + strand, within pstA at 4469.720 kb on - strand, within pstA at 4469.727 kb on + strand, within pstA at 4469.727 kb on + strand, within pstA at 4469.728 kb on - strand, within pstA at 4469.735 kb on - strand, within pstA at 4469.735 kb on - strand, within pstA at 4469.737 kb on + strand, within pstA at 4469.791 kb on - strand, within pstA at 4469.850 kb on + strand, within pstA at 4469.850 kb on + strand, within pstA at 4469.850 kb on + strand, within pstA at 4469.850 kb on + strand, within pstA at 4469.850 kb on + strand, within pstA at 4469.850 kb on + strand, within pstA at 4469.850 kb on + strand, within pstA at 4469.858 kb on - strand, within pstA at 4469.858 kb on - strand, within pstA at 4469.858 kb on - strand, within pstA at 4469.858 kb on - strand, within pstA at 4469.869 kb on + strand, within pstA at 4469.948 kb on + strand, within pstA at 4469.948 kb on + strand, within pstA at 4469.949 kb on + strand, within pstA at 4469.949 kb on + strand, within pstA at 4469.949 kb on + strand, within pstA at 4469.949 kb on + strand, within pstA at 4469.949 kb on + strand, within pstA at 4469.957 kb on - strand, within pstA at 4469.957 kb on - strand, within pstA at 4469.957 kb on - strand, within pstA at 4469.957 kb on - strand, within pstA at 4469.989 kb on - strand at 4469.996 kb on + strand at 4470.005 kb on + strand at 4470.007 kb on - strand at 4470.016 kb on - strand at 4470.036 kb on - strand at 4470.067 kb on + strand at 4470.067 kb on + strand at 4470.067 kb on + strand at 4470.075 kb on - strand at 4470.075 kb on - strand at 4470.075 kb on - strand at 4470.075 kb on - strand at 4470.075 kb on - strand at 4470.075 kb on - strand at 4470.088 kb on + strand at 4470.112 kb on + strand at 4470.117 kb on + strand at 4470.125 kb on - strand at 4470.132 kb on + strand at 4470.132 kb on + strand at 4470.168 kb on - strand at 4470.169 kb on - strand at 4470.174 kb on + strand at 4470.179 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB - with RNA protect remove 4,467,555 + -0.9 4,467,573 + -1.3 4,467,591 - -0.4 4,467,593 + -1.1 4,467,602 - -2.1 4,467,602 - -0.3 4,467,612 + -0.8 4,467,620 - SO4288 0.11 -0.2 4,467,626 - SO4288 0.12 +0.1 4,467,631 - SO4288 0.12 -1.2 4,467,631 - SO4288 0.12 -1.4 4,467,640 + SO4288 0.14 -0.4 4,467,682 - SO4288 0.21 -0.8 4,467,702 + SO4288 0.24 -0.7 4,467,709 + SO4288 0.25 -1.9 4,467,739 - SO4288 0.30 -0.8 4,467,752 - SO4288 0.32 -0.7 4,467,752 - SO4288 0.32 -0.3 4,467,784 + SO4288 0.37 +0.3 4,467,794 + SO4288 0.38 -1.0 4,467,807 - SO4288 0.40 -0.7 4,467,807 - SO4288 0.40 -1.2 4,467,840 - SO4288 0.45 -1.0 4,467,855 + SO4288 0.48 -0.2 4,467,855 + SO4288 0.48 +0.6 4,467,863 - SO4288 0.49 -0.1 4,467,863 - SO4288 0.49 -0.9 4,467,863 - SO4288 0.49 -0.7 4,467,863 - SO4288 0.49 -0.5 4,467,893 + SO4288 0.54 -0.3 4,467,914 + SO4288 0.57 -0.4 4,467,914 + SO4288 0.57 +0.8 4,467,914 + SO4288 0.57 -1.3 4,467,914 + SO4288 0.57 -0.9 4,467,914 + SO4288 0.57 -0.3 4,467,914 + SO4288 0.57 -0.3 4,467,922 - SO4288 0.58 -0.2 4,467,929 - SO4288 0.60 +0.2 4,467,945 + SO4288 0.62 +0.1 4,467,948 - -0.9 4,467,992 - SO4288 0.70 -0.6 4,468,000 - SO4288 0.71 +0.8 4,468,000 - SO4288 0.71 +0.1 4,468,000 - SO4288 0.71 +0.1 4,468,000 - SO4288 0.71 -1.2 4,468,081 - SO4288 0.84 -0.5 4,468,083 + SO4288 0.84 +1.1 4,468,101 + SO4288 0.87 -0.0 4,468,109 - SO4288 0.88 +1.6 4,468,134 + -0.6 4,468,151 + -0.3 4,468,159 - +0.5 4,468,165 + -0.9 4,468,186 + -0.2 4,468,194 - -0.8 4,468,270 - -2.9 4,468,353 + +0.6 4,468,364 + +0.6 4,468,374 + -1.1 4,468,476 - -1.9 4,468,494 + -1.2 4,468,512 - pstB-2 SO4289 0.10 -0.3 4,468,530 - pstB-2 SO4289 0.12 -1.7 4,468,655 + pstB-2 SO4289 0.29 +0.7 4,468,655 + pstB-2 SO4289 0.29 -0.6 4,468,663 - pstB-2 SO4289 0.30 -2.4 4,468,663 - pstB-2 SO4289 0.30 -0.2 4,468,663 - pstB-2 SO4289 0.30 +0.2 4,468,676 - pstB-2 SO4289 0.32 -1.5 4,468,752 + pstB-2 SO4289 0.42 -0.6 4,468,757 + pstB-2 SO4289 0.43 -0.8 4,468,775 - pstB-2 SO4289 0.45 -0.9 4,468,777 + pstB-2 SO4289 0.45 -1.1 4,468,785 - pstB-2 SO4289 0.46 -1.0 4,468,785 - pstB-2 SO4289 0.46 -1.1 4,468,796 + pstB-2 SO4289 0.48 -0.3 4,468,796 + pstB-2 SO4289 0.48 -1.2 4,468,796 + pstB-2 SO4289 0.48 -0.5 4,468,799 - pstB-2 SO4289 0.48 -0.4 4,468,982 - pstB-2 SO4289 0.73 -0.1 4,469,049 + pstB-2 SO4289 0.82 -1.3 4,469,057 - pstB-2 SO4289 0.83 +0.6 4,469,064 + pstB-2 SO4289 0.84 -1.9 4,469,082 + pstB-2 SO4289 0.86 -0.2 4,469,082 + pstB-2 SO4289 0.86 -0.8 4,469,082 + pstB-2 SO4289 0.86 -1.3 4,469,082 + pstB-2 SO4289 0.86 -0.6 4,469,090 - pstB-2 SO4289 0.87 -1.4 4,469,090 - pstB-2 SO4289 0.87 -0.1 4,469,090 - pstB-2 SO4289 0.87 -1.4 4,469,090 - pstB-2 SO4289 0.87 -0.8 4,469,090 - pstB-2 SO4289 0.87 -1.1 4,469,090 - pstB-2 SO4289 0.87 -0.9 4,469,090 - pstB-2 SO4289 0.87 -0.8 4,469,090 - pstB-2 SO4289 0.87 -0.1 4,469,090 - pstB-2 SO4289 0.87 +0.2 4,469,090 - pstB-2 SO4289 0.87 -0.4 4,469,090 - pstB-2 SO4289 0.87 -0.2 4,469,090 - pstB-2 SO4289 0.87 +0.4 4,469,275 - -0.1 4,469,276 - -1.8 4,469,290 + -0.2 4,469,297 - +1.4 4,469,308 + -0.4 4,469,316 - pstA SO4290 0.10 -0.1 4,469,316 - pstA SO4290 0.10 -1.2 4,469,322 - pstA SO4290 0.11 -0.9 4,469,371 + pstA SO4290 0.17 +2.2 4,469,388 + pstA SO4290 0.19 -0.3 4,469,388 + pstA SO4290 0.19 -0.5 4,469,388 + pstA SO4290 0.19 -0.0 4,469,388 + pstA SO4290 0.19 -0.2 4,469,388 + pstA SO4290 0.19 +0.3 4,469,396 - pstA SO4290 0.20 +0.7 4,469,396 - pstA SO4290 0.20 +0.1 4,469,396 - pstA SO4290 0.20 -0.3 4,469,396 - pstA SO4290 0.20 +1.2 4,469,417 + pstA SO4290 0.22 +0.2 4,469,417 + pstA SO4290 0.22 -0.7 4,469,430 + pstA SO4290 0.24 -0.3 4,469,458 - pstA SO4290 0.27 -0.1 4,469,462 + pstA SO4290 0.28 +1.5 4,469,463 + pstA SO4290 0.28 +0.5 4,469,471 - pstA SO4290 0.29 -0.2 4,469,496 + pstA SO4290 0.32 +0.4 4,469,504 - pstA SO4290 0.33 +0.4 4,469,555 - pstA SO4290 0.39 -0.3 4,469,559 + pstA SO4290 0.39 +0.1 4,469,564 + pstA SO4290 0.40 +0.6 4,469,574 + pstA SO4290 0.41 -0.3 4,469,582 + pstA SO4290 0.42 -0.5 4,469,582 - pstA SO4290 0.42 -1.3 4,469,582 - pstA SO4290 0.42 -1.5 4,469,589 + pstA SO4290 0.43 -1.2 4,469,589 + pstA SO4290 0.43 -1.5 4,469,589 + pstA SO4290 0.43 -0.1 4,469,589 + pstA SO4290 0.43 -0.8 4,469,589 + pstA SO4290 0.43 -0.1 4,469,589 + pstA SO4290 0.43 -1.9 4,469,589 + pstA SO4290 0.43 -0.2 4,469,589 + pstA SO4290 0.43 -1.5 4,469,589 + pstA SO4290 0.43 -0.3 4,469,595 + pstA SO4290 0.44 -0.5 4,469,597 - pstA SO4290 0.44 -0.8 4,469,597 - pstA SO4290 0.44 -0.1 4,469,597 - pstA SO4290 0.44 -0.9 4,469,597 - pstA SO4290 0.44 -0.9 4,469,597 - pstA SO4290 0.44 +0.0 4,469,597 - pstA SO4290 0.44 -0.2 4,469,600 + pstA SO4290 0.44 -0.8 4,469,608 - pstA SO4290 0.45 -1.4 4,469,608 - pstA SO4290 0.45 -0.5 4,469,669 + pstA SO4290 0.53 -1.8 4,469,669 + pstA SO4290 0.53 +0.1 4,469,720 - pstA SO4290 0.59 -0.1 4,469,727 + pstA SO4290 0.59 -0.6 4,469,727 + pstA SO4290 0.59 -1.7 4,469,728 - pstA SO4290 0.60 -1.6 4,469,735 - pstA SO4290 0.60 -1.4 4,469,735 - pstA SO4290 0.60 -0.1 4,469,737 + pstA SO4290 0.61 +0.8 4,469,791 - pstA SO4290 0.67 -1.0 4,469,850 + pstA SO4290 0.74 -0.1 4,469,850 + pstA SO4290 0.74 -1.9 4,469,850 + pstA SO4290 0.74 -0.0 4,469,850 + pstA SO4290 0.74 -0.3 4,469,850 + pstA SO4290 0.74 +0.6 4,469,850 + pstA SO4290 0.74 +0.0 4,469,850 + pstA SO4290 0.74 +0.9 4,469,858 - pstA SO4290 0.75 +1.1 4,469,858 - pstA SO4290 0.75 -0.4 4,469,858 - pstA SO4290 0.75 -1.8 4,469,858 - pstA SO4290 0.75 -0.5 4,469,869 + pstA SO4290 0.76 -0.4 4,469,948 + pstA SO4290 0.86 -0.0 4,469,948 + pstA SO4290 0.86 +1.3 4,469,949 + pstA SO4290 0.86 +0.7 4,469,949 + pstA SO4290 0.86 +0.3 4,469,949 + pstA SO4290 0.86 +0.4 4,469,949 + pstA SO4290 0.86 -0.6 4,469,949 + pstA SO4290 0.86 +0.7 4,469,957 - pstA SO4290 0.87 +0.9 4,469,957 - pstA SO4290 0.87 +0.6 4,469,957 - pstA SO4290 0.87 -0.9 4,469,957 - pstA SO4290 0.87 -0.4 4,469,989 - -0.9 4,469,996 + -0.4 4,470,005 + -0.5 4,470,007 - -0.5 4,470,016 - -0.0 4,470,036 - +0.9 4,470,067 + -0.0 4,470,067 + -1.1 4,470,067 + -1.8 4,470,075 - -1.2 4,470,075 - +0.4 4,470,075 - -0.3 4,470,075 - +0.1 4,470,075 - +0.1 4,470,075 - -1.0 4,470,088 + -0.0 4,470,112 + +1.2 4,470,117 + -1.0 4,470,125 - +0.6 4,470,132 + +0.5 4,470,132 + +0.0 4,470,168 - +0.1 4,470,169 - +0.3 4,470,174 + +0.1 4,470,179 - -0.7
Or see this region's nucleotide sequence