Experiment: LB - with RNA protect
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mutY and SO3369 are separated by 9 nucleotides SO3369 and SO3370 are separated by 80 nucleotides SO3370 and SO3371 are separated by 4 nucleotides SO3371 and tRNA-Phe-3 are separated by 387 nucleotides tRNA-Phe-3 and tRNA-Thr-5 are separated by 21 nucleotides
SO3368: mutY - A/G-specific adenine glycosylase (NCBI ptt file), at 3,510,656 to 3,511,753
mutY
SO3369: SO3369 - conserved hypothetical protein (NCBI ptt file), at 3,511,763 to 3,512,041
SO3369
SO3370: SO3370 - conserved hypothetical protein (NCBI ptt file), at 3,512,122 to 3,512,697
SO3370
SO3371: SO3371 - hypothetical Cytochrome B561 (NCBI ptt file), at 3,512,702 to 3,513,253
SO3371
SO_t079: tRNA-Phe-3 - tRNA-Phe (RefSeq), at 3,513,641 to 3,513,716
tRNA-Phe-3
SO_t080: tRNA-Thr-5 - tRNA-Thr (RefSeq), at 3,513,738 to 3,513,813
tRNA-Thr-5
Position (kb)
3512
3513
3514 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3511.882 kb on - strand, within SO3369 at 3511.904 kb on + strand, within SO3369 at 3511.992 kb on + strand, within SO3369 at 3512.048 kb on + strand at 3512.056 kb on - strand at 3512.056 kb on - strand at 3512.157 kb on - strand at 3512.163 kb on + strand at 3512.163 kb on + strand at 3512.170 kb on + strand at 3512.249 kb on + strand, within SO3370 at 3512.257 kb on - strand, within SO3370 at 3512.263 kb on + strand, within SO3370 at 3512.402 kb on + strand, within SO3370 at 3512.428 kb on - strand, within SO3370 at 3512.495 kb on + strand, within SO3370 at 3512.495 kb on + strand, within SO3370 at 3512.509 kb on - strand, within SO3370 at 3512.509 kb on - strand, within SO3370 at 3512.560 kb on + strand, within SO3370 at 3512.568 kb on - strand, within SO3370 at 3512.568 kb on - strand, within SO3370 at 3512.568 kb on - strand, within SO3370 at 3512.568 kb on - strand, within SO3370 at 3512.568 kb on - strand, within SO3370 at 3512.587 kb on + strand, within SO3370 at 3512.671 kb on - strand at 3512.732 kb on - strand at 3512.734 kb on + strand at 3512.734 kb on + strand at 3512.742 kb on - strand at 3512.786 kb on - strand, within SO3371 at 3512.804 kb on + strand, within SO3371 at 3512.804 kb on + strand, within SO3371 at 3512.825 kb on + strand, within SO3371 at 3512.871 kb on - strand, within SO3371 at 3512.904 kb on - strand, within SO3371 at 3512.986 kb on - strand, within SO3371 at 3513.038 kb on - strand, within SO3371 at 3513.117 kb on + strand, within SO3371 at 3513.117 kb on + strand, within SO3371 at 3513.117 kb on + strand, within SO3371 at 3513.117 kb on + strand, within SO3371 at 3513.117 kb on + strand, within SO3371 at 3513.120 kb on - strand, within SO3371 at 3513.125 kb on - strand, within SO3371 at 3513.125 kb on - strand, within SO3371 at 3513.125 kb on - strand, within SO3371 at 3513.125 kb on - strand, within SO3371 at 3513.182 kb on + strand, within SO3371 at 3513.192 kb on + strand, within SO3371 at 3513.195 kb on - strand, within SO3371 at 3513.268 kb on + strand at 3513.276 kb on - strand at 3513.396 kb on + strand at 3513.404 kb on - strand at 3513.449 kb on - strand at 3513.449 kb on - strand at 3513.551 kb on + strand at 3513.594 kb on + strand at 3513.890 kb on + strand at 3513.931 kb on + strand at 3513.932 kb on - strand at 3513.955 kb on - strand at 3514.031 kb on - strand at 3514.120 kb on - strand at 3514.142 kb on - strand at 3514.235 kb on + strand at 3514.243 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB - with RNA protect remove 3,511,882 - SO3369 0.43 -0.3 3,511,904 + SO3369 0.51 -0.1 3,511,992 + SO3369 0.82 -0.9 3,512,048 + +0.0 3,512,056 - -1.5 3,512,056 - +0.5 3,512,157 - -0.3 3,512,163 + +1.1 3,512,163 + +0.2 3,512,170 + -0.7 3,512,249 + SO3370 0.22 -0.6 3,512,257 - SO3370 0.23 +0.8 3,512,263 + SO3370 0.24 -1.2 3,512,402 + SO3370 0.49 -0.3 3,512,428 - SO3370 0.53 -0.6 3,512,495 + SO3370 0.65 +0.9 3,512,495 + SO3370 0.65 +0.2 3,512,509 - SO3370 0.67 -0.2 3,512,509 - SO3370 0.67 +0.8 3,512,560 + SO3370 0.76 +0.5 3,512,568 - SO3370 0.77 -0.4 3,512,568 - SO3370 0.77 -0.1 3,512,568 - SO3370 0.77 +1.0 3,512,568 - SO3370 0.77 -0.1 3,512,568 - SO3370 0.77 +1.3 3,512,587 + SO3370 0.81 -1.0 3,512,671 - -0.1 3,512,732 - -1.9 3,512,734 + -0.4 3,512,734 + -0.2 3,512,742 - +0.8 3,512,786 - SO3371 0.15 -0.4 3,512,804 + SO3371 0.18 -0.6 3,512,804 + SO3371 0.18 +0.5 3,512,825 + SO3371 0.22 -0.1 3,512,871 - SO3371 0.31 -0.1 3,512,904 - SO3371 0.37 -0.2 3,512,986 - SO3371 0.51 +0.5 3,513,038 - SO3371 0.61 -1.0 3,513,117 + SO3371 0.75 -0.9 3,513,117 + SO3371 0.75 -0.2 3,513,117 + SO3371 0.75 -1.2 3,513,117 + SO3371 0.75 -0.3 3,513,117 + SO3371 0.75 +0.2 3,513,120 - SO3371 0.76 -0.9 3,513,125 - SO3371 0.77 +1.0 3,513,125 - SO3371 0.77 -0.5 3,513,125 - SO3371 0.77 -1.1 3,513,125 - SO3371 0.77 -1.3 3,513,182 + SO3371 0.87 +0.2 3,513,192 + SO3371 0.89 -2.1 3,513,195 - SO3371 0.89 +1.1 3,513,268 + +0.1 3,513,276 - +1.0 3,513,396 + +0.4 3,513,404 - -0.1 3,513,449 - -0.2 3,513,449 - +0.3 3,513,551 + +0.8 3,513,594 + -0.2 3,513,890 + +0.6 3,513,931 + -0.9 3,513,932 - -1.6 3,513,955 - -1.1 3,514,031 - -1.1 3,514,120 - -0.8 3,514,142 - -3.4 3,514,235 + +0.1 3,514,243 - +1.7
Or see this region's nucleotide sequence