Experiment: LB - with RNA protect
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO3108 and SO3109 are separated by 335 nucleotides SO3109 and secF-2 are separated by 147 nucleotides secF-2 and secD-2 are separated by 13 nucleotides
SO3108: SO3108 - siroheme synthase, N-terminal component, putative (NCBI ptt file), at 3,236,703 to 3,237,614
SO3108
SO3109: SO3109 - conserved hypothetical protein (NCBI ptt file), at 3,237,950 to 3,238,381
SO3109
SO3110: secF-2 - protein-export membrane protein SecF (NCBI ptt file), at 3,238,529 to 3,239,476
secF-2
SO3111: secD-2 - protein-export membrane protein SecD (NCBI ptt file), at 3,239,490 to 3,241,340
secD-2
Position (kb)
3238
3239
3240 Strain fitness (log2 ratio)
-2
-1
0
1 at 3237.616 kb on + strand at 3237.624 kb on - strand at 3237.676 kb on - strand at 3237.694 kb on - strand at 3237.798 kb on + strand at 3237.908 kb on - strand at 3238.002 kb on - strand, within SO3109 at 3238.062 kb on - strand, within SO3109 at 3238.111 kb on + strand, within SO3109 at 3238.121 kb on + strand, within SO3109 at 3238.129 kb on - strand, within SO3109 at 3238.129 kb on - strand, within SO3109 at 3238.137 kb on + strand, within SO3109 at 3238.207 kb on - strand, within SO3109 at 3238.215 kb on + strand, within SO3109 at 3238.403 kb on + strand at 3238.654 kb on + strand, within secF-2 at 3239.045 kb on + strand, within secF-2 at 3239.180 kb on + strand, within secF-2 at 3239.275 kb on - strand, within secF-2 at 3239.295 kb on - strand, within secF-2 at 3239.400 kb on - strand at 3239.407 kb on + strand at 3239.407 kb on + strand at 3239.495 kb on - strand at 3239.706 kb on - strand, within secD-2 at 3239.807 kb on - strand, within secD-2 at 3239.829 kb on + strand, within secD-2 at 3239.829 kb on + strand, within secD-2 at 3239.829 kb on + strand, within secD-2 at 3239.837 kb on - strand, within secD-2 at 3239.837 kb on - strand, within secD-2 at 3239.837 kb on - strand, within secD-2 at 3239.837 kb on - strand, within secD-2 at 3239.967 kb on - strand, within secD-2 at 3239.967 kb on - strand, within secD-2 at 3240.013 kb on - strand, within secD-2 at 3240.081 kb on + strand, within secD-2 at 3240.128 kb on - strand, within secD-2 at 3240.315 kb on - strand, within secD-2 at 3240.380 kb on + strand, within secD-2
Per-strain Table
Position Strand Gene LocusTag Fraction LB - with RNA protect remove 3,237,616 + -0.0 3,237,624 - +1.0 3,237,676 - -0.7 3,237,694 - +1.4 3,237,798 + -1.6 3,237,908 - +0.0 3,238,002 - SO3109 0.12 +0.1 3,238,062 - SO3109 0.26 +0.2 3,238,111 + SO3109 0.37 +0.1 3,238,121 + SO3109 0.40 -2.1 3,238,129 - SO3109 0.41 +1.0 3,238,129 - SO3109 0.41 -1.4 3,238,137 + SO3109 0.43 +1.1 3,238,207 - SO3109 0.59 -0.4 3,238,215 + SO3109 0.61 -0.0 3,238,403 + +0.4 3,238,654 + secF-2 SO3110 0.13 -0.2 3,239,045 + secF-2 SO3110 0.54 -0.8 3,239,180 + secF-2 SO3110 0.69 +1.4 3,239,275 - secF-2 SO3110 0.79 -0.8 3,239,295 - secF-2 SO3110 0.81 -1.8 3,239,400 - -0.6 3,239,407 + -0.4 3,239,407 + +0.1 3,239,495 - -0.2 3,239,706 - secD-2 SO3111 0.12 +0.0 3,239,807 - secD-2 SO3111 0.17 -1.5 3,239,829 + secD-2 SO3111 0.18 -0.4 3,239,829 + secD-2 SO3111 0.18 -0.8 3,239,829 + secD-2 SO3111 0.18 -0.0 3,239,837 - secD-2 SO3111 0.19 +0.8 3,239,837 - secD-2 SO3111 0.19 +0.3 3,239,837 - secD-2 SO3111 0.19 -0.1 3,239,837 - secD-2 SO3111 0.19 -1.2 3,239,967 - secD-2 SO3111 0.26 -0.3 3,239,967 - secD-2 SO3111 0.26 -0.5 3,240,013 - secD-2 SO3111 0.28 +0.4 3,240,081 + secD-2 SO3111 0.32 -0.3 3,240,128 - secD-2 SO3111 0.34 +0.4 3,240,315 - secD-2 SO3111 0.45 +0.6 3,240,380 + secD-2 SO3111 0.48 -1.2
Or see this region's nucleotide sequence